RIF1 antibody

Principal name

RIF1 antibody

Alternative names for RIF1 antibody

Rap1-interacting factor 1 homolog, Telomere-associated protein RIF1

SwissProt ID

P29539 (Yeast), Q5UIP0 (Human), Q6PR54 (Mouse), Q96UP3 (Schpo), Q9H830 (Human)

Gene ID

55183 (RIF1), 51869 (Rif1)

Available reactivities

Hu (Human), Ms (Mouse), Chimp (Chimpanzee), Rt (Rat), Can (Canine), Bov (Bovine), Por (Porcine)

Available hosts

Rabbit, Goat

Available applications

Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of RIF1 antibody

Rif1 (also known as telomere-associated protein RIF1 and Rap1-interacting factor 1) is a mouse ortholog of the yeast Rif1 family of telomere-associated proteins identified on the basis of its high expression in primordial germ cells and embryo-derived pluripotent stem cell lines. Mouse Rif1 is a protein involved in negative regulation of telomere length. It is also localized to ATM dependent DNA damage foci and may act at the chromatin level to allow access to some factors.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RIF1 (C-term) antibody

Data provided by customer: (1ug/ml) staining of HeLa and Mouse D3 (ES) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RIF1 (2406-2419) antibody

WB using Anti-Rif1 antibody shows detection of a band ~265 kDa corresponding to mouse Rif1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). The primary antibody diluted to 1.0ug/ml. IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX  was used at 1:10000. Rabbit IgG Aff - Purified Can, Chimp, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RIF1 (2406-2419) antibody

Formalin-Fixed Paraffin-Embedded Human Testis stained with Rif1 antibody Cat.-No AP07403PU-N Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RIF1 antibody

RIF1 Goat Hu E
0.1 mg / €390.00
  Abnova Taiwan Corp.

RIF1 antibody

Western blot using Rif1 polyclonal antibody ( Cat # PAB10275 ) shows detection of a band ~ 265 kDa corresponding to mouse Rif1 ( arrowhead ).
Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide ( data not shown ).
Approximately 25 µg of MEF whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1.0 µg/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDyeTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Rabbit Chimp, Hu, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

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