RIPK3 / RIP3 antibody

Principal name

RIPK3 / RIP3 antibody

Alternative names for RIPK3 / RIP3 antibody

RIP-3, Receptor-interacting serine/threonine-protein kinase 3, RIP-like protein kinase 3, Receptor-interacting protein 3

SwissProt ID

Q9QZL0 (Mouse), Q9Y572 (Human), Q9Z2P5 (Rat)

Gene ID

11035 (RIPK3), 56532 (Ripk3)

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human), Chimp (Chimpanzee), Bov (Bovine), Can (Canine), Mky (Monkey), Prim (Primate)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of RIPK3 / RIP3 antibody

Receptor interacting serine/threonine kinase protein 3 (RIP3) is a member of the RIP kinase family. It is expressed in the embryo and in multiple adult tissues, including most hemopoietic cell lineages. RIP3 is a member of the RIP kinase family which is believed to be dispensable for normal development of mouse embryos (1). The RIP3 deficient cells exhibit normal sensitivity to a variety of apoptotic stimuli and were indistinguishable from wild type cells in their ability to activate NF-kB signaling in response to the TNFF and LPS (1). What role if any RIP3 plays in the regulation of apoptosis and N-kB signaling by activation or attenuation of NF-kB family of transcription factors is not clearly understood and still controversial. RIP3 interacts with serine/threonine kinase RIP with an N-terminal kinases domain as found in RIP and RIP2 kinases. The RIP posses C-terminal death domain, while RIP2 has a C-terminal caspase activation domain and RIP3 has a unique C-terminus. The murine herpesviruses such as cytomegalovirus M45 protein interacts with receptor-interacting protein (RIP) 1 and RIP3 via a RIP homotypic interaction motif.
Programmed necrosis is a form of caspase-independent cell death whose molecular regulation is poorly understood. The kinase RIP1 is crucial for programmed necrosis, but also mediates activation of the prosurvival transcription factor NF-kappaB. The necrosis can be induced by stimulating death receptors with TNF or other death receptor agonists. The RIP3 is the molecular switch that is required for necrosis in NIH 3T3 cells followed by TNF treatment. RIP3 did not affect RIP1 mediated apoptosis but was required for RIP1 mediated necrosis. RIP3 also regulates TNF-induced reactive oxygen species production that partially accounts for RIP3’s ability to promote necrosis (2). RIP3 regulates necrosis-specific RIP1 phosphorylation, and the phosphorylation of RIP1 and RIP3 stabilizes their association within the pronectrotic complex, that activates the pronecrtic kinase activity that triggers the downstream reactive oxygen species production. RIP3 controls programmed necrosis by initiating the pronecrotic kinases cascade and is necessary for the inflammatory response against viral infections (3). RIP3 is a nucleocytoplasmic shuttling protein and its unconventional nuclear localization signal (NLS, 442-472 aa) is sufficient to trigger apoptosis in the nucleus in many cells. This NLS motif is involved in RIP-RIP3 interaction, is necessary for RIP3 self association and for RIP3 induced apoptosis and RIP3 induced NF-kB activation (4). Several splice variant of this protein has been sequences based on conceptual translation.
The RIPk3 protein is a approximately 518 amino acid (64kDa) protein.

General readings

Yu, P.W. et al., Curr. Biol. 9: 539-542 (1999)
Sun, X. et al., J. Biol. Chem. 274: 16871-16875 (1999)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RIPK3 / RIP3 antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RIPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803093) Mouse IgG1 OTI2B10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIPK3 / RIP3 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RIPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH9.0, 120C for 3min, TA803100) Mouse IgG2a OTI1B3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIPK3 / RIP3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RIPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803231) Mouse IgG2b OTI3A3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIPK3 / RIP3 antibody

Western blot analysis of RIP3 in mouse 3T3 whole cell lysate in the absence (A) or presence (B) of blocking peptide with RIP3 antibody at 1 ug/ml. Rabbit IgG Aff - Purified Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RIPK3 / RIP3 antibody

Western Blot: RIPK3 Antibody [TA337010] - analysis of RIP3 using RIP3 polyclonal antibody. human tissue blot probed with 5 ug/ml of RIP3 antibody: lanes 1) brain, 2) heart, 3) small intestine, 4) kidney, 5) liver, 6) lung, 7) skeletal muscle, 8) stomach, Rabbit Purified Hu, Prim ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RIPK3 / RIP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3 (RC209549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPK3. Mouse IgG2a OTI2F11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIPK3 / RIP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3 (RC209549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPK3. Mouse IgG1 OTI2H8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIPK3 / RIP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3 (RC209549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPK3. Mouse IgG1 OTI2C11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIPK3 / RIP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3 (RC209549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPK3. Mouse IgG1 OTI3A12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIPK3 / RIP3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RIPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803171) Mouse IgG1 OTI2F12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIPK3 / RIP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3 (RC209549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPK3. Mouse IgG1 OTI1H1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIPK3 / RIP3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3 (RC209549, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPK3. Mouse IgG2b OTI1D9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIPK3 / RIP3 control peptide

RIPK3 / RIP3
0.25 mg / €250.00
  Acris Antibodies GmbH

RIPK3 / RIP3 (498-518) antibody

RIPK3 / RIP3 Rabbit IgG Aff - Purified Hu, Mky IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

RIPK3 / RIP3 (N-term) antibody

Immunohistochemistry analysis in human lung carcinoma tissue (Formalin-fixed, Paraffin-embedded) using RIPK3 / RIP3 antibody Cat.-No. AP13846PU-N (N-term), followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RIPK3 / RIP3 (Center) antibody

Immunohistochemistry analysis in human lung carcinoma tissue (Formalin-fixed, Paraffin-embedded) using  RIPK3 / RIP3 antibody Cat.-No. AP13847PU-N, followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RIPK3 / RIP3 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RIPK3 / RIP3 Control Peptide

RIPK3 / RIP3
50 µg / €160.00
  Acris Antibodies GmbH

RIPK3 / RIP3 antibody

Detection of human RIPK3. HEK 293T cells ( 5 X 105 ) mock transfected ( lane 1 ) or transfected ( lane 2 ) with an expression plasmid coding for human RIPK3. Incubated with the RIPK3 monoclonal antibody, clone Rippy-3 ( Cat # MAB3728 ) at a 1 : 1,000 dilution. Mouse IgG1 Rippy-3 Hu WB
0.1 mg / €730.00
  Abnova Taiwan Corp.

RIPK3 / RIP3 antibody

IHC analysis of human pancreas. Using RIPK3 polyclonal antibody ( Cat # PAB0287 ) at 10 µg/ml. Rabbit Chimp, Hu C, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

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