RIT2 antibody

Principal name

RIT2 antibody

Alternative names for RIT2 antibody

RIN, ROC2, GTP-binding protein Rit2, Ras-like protein expressed in neurons, Ras-like without CAAX protein 2

SwissProt ID

P70425 (Mouse), Q5BJQ5 (Rat), Q99578 (Human)

Gene ID

6014 (RIT2)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Mky (Monkey), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of RIT2 antibody

The Ras super-family comprises a group of structurally related low molecular weight (20-30 kDa) proteins, which bind GTP or GDP and exhibit a low intrinsic GTPase activity. They are involved in signal transduction and the regulation of a variety of cellular processes, including cell growth, transformation, differentiation and morphogenesis, nucleocytoplasmic transport, and apoptosis. Ras proteins promote cancer by disrupting the normal controls of cell proliferation and differentiation. All Ras subgroups (Ras, Rho, Rab, Ran, and ADP ribosylation factor) contain five highly conserved domains (G1-G5) and act as molecular switches by alternating between an active GTP-bound form and an inactive GDP-bound form. Rit2 (Ras-like protein in neurons) and Rit1 (Ras-like protein in tissues) share 50% homology to Ras. Both contain the five highly conserved domains (G1-G5), bind GTP in vitro, and are membrane-associated. However, both lack a CAAX box and their mechanism of membrane association is distinct from the typical Ras protein. Rit is a non-lipid-modified Ras-related protein, which lacks any known recognition signal for C-terminal lipidation. Rit1 and Rit2 share a unique effector domain (DPTIEDAYK) that is 100% conserved between murine and human proteins. Rit1 is expressed ubiquitously, but Rit2 is expressed only in subsets of neurons and may be involved in calcium-mediated signaling within neurons. Rit uses novel effector pathways to regulate proliferation and transformation. It has been suggested that Rit1 and Rit2 play important role in the regulation of signaling pathways, distinct from those controlled by Ras. Ras and associated pathways have also been implicated in the development and maintenance of themammalian retina.

General readings

General / background references:Rusyn EV et al. Rit, a non-lipid-modified Ras-related protein, transforms NIH3T3 cells without activating the ERK, JNK, p38 MAPK or PI3K/Akt pathways. Oncogene 19:4685-94 (2000). PubMed PMID: 11032018
Shao H et al. Biochemical characterization of the Ras-related GTPases Rit and Rin. Arch Biochem Biophys 371:207-19 (1999). PubMed PMID: 10545207
Lee CH et al. Rin, a neuron-specific and calmodulin-binding small G-protein, and Rit define a novel subfamily of ras proteins. J Neurosci 16:6784-94 (1996). PubMed PMID: 8824319

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RIT2 antibody

RIT2 Mouse IgG2b OTI4B5 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIT2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501757, Dilution 1:50) Mouse IgG2a OTI3F4 Purified Can, Hu, Mky, Rt F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RIT2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIT2 (RC205367, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIT2. Mouse IgG2a OTI4A6 Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIT2 antibody

Anti-RIT2 mouse monoclonal antibody (TA501703) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIT2(RC205367). Mouse IgG1 OTI1G2 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RIT2 antibody

Figure 1. Western blot analysis using Rin antibody Cat.-No AM05291PU-N on 293 cells expressing HA-tagged Rit (Lane 1) and HA-tagged Rin (Lane 2). Mouse IgG2b 27G2 Purified Hu, Ms P, WB
0.1 mg / €360.00
  Acris Antibodies GmbH

RIT2 antibody

RIT2 Rabbit IgG Purified Hu WB
0.1 mg / €360.00
  Acris Antibodies GmbH

RIT2 (N-term) antibody

Western blot analysis of RIT2 Antibody (N-term) Cat.-No AP53671PU-N in A549 cell line lysates (35ug/lane). This demonstrates the RIT2 antibody detected the RIT2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RIT2 antibody

Western Blot analysis of RIT2 expression in transfected 293T cell line (H00006014-T01) by RIT2 MaxPab polyclonal antibody.

Lane 1: RIT2 transfected lysate(23.87 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RIT2 antibody

Western Blot analysis of RIT2 expression in transfected 293T cell line (H00006014-T02) by RIT2 MaxPab polyclonal antibody.

Lane 1: RIT2 transfected lysate(23.87 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RIT2 antibody

Detection limit for recombinant GST tagged RIT2 is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 3F2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RIT2 antibody

Western blot analysis using RIT2 monoclonal antibody, clone 27G2 ( Cat # MAB2012 ) on 293 cells expressing HA-tagged RIT1 (1) and HA-tagged RIT2 (2). Hu, Ms
0.1 mg / €400.00
  Abnova Taiwan Corp.

RIT2 antibody

RIT2 Rabbit IgG Purified Hu WB
50 µg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RIT2

RIT2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

RIT2

RIT2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RIT2

RIT2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RIT2 293T Cell Transient Overexpression Lysate(Denatured)

RIT2 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

RIT2 overexpression lysate

RIT2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

17 Item(s)

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