RNF12 / RLIM antibody

Principal name

RNF12 / RLIM antibody

Alternative names for RNF12 / RLIM antibody

R-LIM, NY-REN-43, E3 ubiquitin-protein ligase RLIM, LIM domain-interacting RING finger protein, RING finger LIM domain-binding protein, RING finger protein 12, Renal carcinoma antigen NY-REN-43

SwissProt ID

Q9NVW2 (Human), Q9WTV7 (Mouse)

Gene ID

51132 (RLIM)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of RNF12 / RLIM antibody

RLIM, also known as RNF12 (RING finger protein 12) or NY-REN-43, is a 624 amino acid RING-H2 zinc finger protein that is involved in protein ubiquitinylation and subsequent degradation. Expressed in a variety of tissues, RLIM binds to the LIM domain of various proteins and functions as a protein ligase that negatively co-regulates LIM homeodomain (LIM-HD) transcription factors. Through its interaction with Sin3A, a component of the histone deacetylase corepressor complex, RLIM is able to recruit the corepressor complex to LIM-HD proteins, thereby inhibiting LIM-HD transcription. In addition to recruiting the deacetylase complex to LIM-HD proteins, RLIM is able to bind to, ubiquinate and subsequently degrade CLIM proteins, which function as positive co-regulators of LIM-HD transcription factors. RLIM contains one RING-type zinc finger and is implicated in renal cell carcinoma.

General readings

Hiratani, I.,et al., (2003) Development 130 (17), 4161-4175

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  2. Highly Validated (1)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RNF12 / RLIM antibody

Immunofluorescence of monoclonal antibody to RNF12 on HeLa cell. [antibody concentration 30 ug/ml] Mouse IgG2a 1G10 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RNF12 / RLIM antibody

WB Suggested Anti-RNF12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HT1080 cell lysateRLIM is supported by BioGPS gene expression data to be expressed in HT1080 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

RNF12 / RLIM antibody

WB Suggested Anti-RNF12 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateRLIM is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

RNF12 / RLIM antibody

RNF12 / RLIM Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RNF12 / RLIM antibody

Western Blot analysis of RNF12 expression in transfected 293T cell line (H00051132-T01) by RNF12 MaxPab polyclonal antibody.

Lane 1: RNF12 transfected lysate(68.64 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RNF12 / RLIM antibody

RNF12 / RLIM Rabbit Serum Hu IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

RNF12 / RLIM antibody

Western Blot analysis of RNF12 expression in transfected 293T cell line (H00051132-T02) by RNF12 MaxPab polyclonal antibody.

Lane 1: RNF12 transfected lysate(68.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RNF12 / RLIM antibody

Human HT1080; WB Suggested Anti-RNF12 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HT1080 cell lysate. RLIM is supported by BioGPS gene expression data to be expressed in HT1080.; RNF12 antibody - N-terminal region (ARP32150_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

RNF12 / RLIM antibody

Human Spermatophore; RNF12 antibody - C-terminal region (ARP32151_P050) in Human Spermatophore cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

RNF12 / RLIM antibody

Immunohistochemistry-Paraffin: RNF12 Antibody [NBP1-89571] - Immunohistochemical staining of human colon shows cytoplasmic and nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RNF12 / RLIM (full length, N-term HIS tag, transcript variant 2)

RNF12 / RLIM Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

RNF12 / RLIM

RNF12 / RLIM Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RNF12 / RLIM

RNF12 / RLIM Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

RNF12 / RLIM

RNF12 / RLIM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RNF12 / RLIM

RNF12 / RLIM Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

RNF12 (Human) IP-WB Antibody Pair

RNF12 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

RNF12 (Human) IP-WB Antibody Pair

RNF12 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RNF12 293T Cell Transient Overexpression Lysate(Denatured)

RNF12 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

18 Item(s)

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