RNF25 antibody

Principal name

RNF25 antibody

Alternative names for RNF25 antibody

RING finger protein 25, E3 ubiquitin-protein ligase RNF25

SwissProt ID

Q5E9N3 (Bovin), Q96BH1 (Human), Q9QZR0 (Mouse)

Gene ID

64320 (RNF25), 5764320 (acpS)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of RNF25 antibody

Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF25 (RING finger protein 25), also known as AO7, is a 459 amino acid protein that contains one RWD domain and one RING-type zinc finger. Via its RING-type zinc finger (a common domain that contains a conserved cysteine-rich region and is found in a number of viral and eukaryotic proteins), RNF25 is thought to exhibit E2-dependent E3 ubiquitin-protein ligase activity. Specifically, the RING finger of RNF25 interacts with an E2 ligase and, through this interaction, facilitates the ubiquitination of target proteins. RNF25 may also regulate the transcriptional activity of NF-kappa-B, a protein that plays important roles in cell survival, inflammation response, host defense and proliferation.

General readings

Asamitsu,K., (2003) J. Biol. Chem. 278 (29), 26879-26887

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RNF25 antibody

Western blot using affinity purified anti-RNF25 antibody shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 1 is a vector only control. The extracts were loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD. Rabbit IgG Aff - Purified Hu, Ms E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RNF25 antibody

WB Suggested Anti-RNF25 Antibody Titration: 0.5 ug/ml; Positive Control: HepG2 cell lysateRNF25 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

RNF25 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, Cerebellum. Rabbit Aff - Purified Hu E, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RNF25 antibody

Human Adipocytes: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

RNF25 antibody

Western Blot analysis of RNF25 expression in transfected 293T cell line (H00064320-T01) by RNF25 MaxPab polyclonal antibody.

Lane 1: RNF25 transfected lysate(50.49 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

RNF25 antibody

RNF25 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

RNF25 antibody

RNF25 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RNF25 antibody

Immunoperoxidase of monoclonal antibody to RNF25 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml] Mouse IgG1 1D10 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RNF25 antibody

Western blot using RNF25 polyclonal antibody ( Cat # PAB11256 ) shows detection of RNF25 ( arrow head ) in HEK293 cells over-expressing human RNF25 ( lane 1 ) or mouse RNF25 ( lane 2 ). Lane 3 is a vector only control. The primary antibody diluted to 1 : 1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD. Rabbit Hu, Ms E, IP, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

RNF25 antibody

Human Skin; RNF25 antibody - middle region (ARP43300_P050) in Human Skin cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

RNF25 antibody

Western Blot: RNF25 Antibody [NBP1-31491] - Western blot analysis of NBP1-31491 on H1299, with 10% SDS PAGE, diluted at 1:1000. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

RNF25 antibody

Western Blot: RNF25 Antibody [NBP1-55063] - This Anti-RNF25 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 0.5ug/ml. Rabbit Aff - Purified Can, Hu P, WB
50 µg / €440.00
  Novus Biologicals Inc.

RNF25 antibody

Immunohistochemistry-Paraffin: RNF25 Antibody [NBP1-89069] - Immunohistochemical staining of human testis shows cytoplasmic positivity in leydig cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RNF25

RNF25 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

RNF25

RNF25 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RNF25

RNF25 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

RNF25

RNF25 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RNF25

RNF25 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

RNF25 (Human) IP-WB Antibody Pair

RNF25 (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RNF25 293T Cell Transient Overexpression Lysate(Denatured)

RNF25 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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