ROBO1 antibody

Principal name

ROBO1 antibody

Alternative names for ROBO1 antibody

ROBO-1, Roundabout homolog 1, DUTT1, Deleted in U Twenty Twenty

SwissProt ID

O55005 (Rat), O89026 (Mouse), Q9Y6N7 (Human)

Gene ID

58946 (Robo1), 6091 (ROBO1), 19876

Available reactivities

Rt (Rat), Hu (Human), Ms (Mouse), Can (Canine), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Bov (Bovine)

Available hosts

Goat, Mouse, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of ROBO1 antibody

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. In Drosophila, the roundabout (robo) gene, a member of the immunoglobulin gene superfamily, encodes an integral membrane protein that is both an axon guidance receptor and a cell adhesion receptor for the SLIT1 and SLIT2 proteins. This receptor is involved in the decision by axons to cross the central nervous system midline. Robo1 is structurally similar to the Drosophila roundabout protein. Robo1 is a widely expressed protein, with the exception of the kidney. Robo1 may be required for lung development and defects in this protein may be a cause of breast and lung cancer. This product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognise native protein in any application.

General readings

1. Andrews W, Liapi A, Plachez C, Camurri L, Zhang J, Mori S, Murakami F, Parnavelas JG, Sundaresan V, Richards LJ.
Robo1 regulates the development of major axon tracts and interneuron migration in the forebrain. Development. 2006 Jun;133(11):2243-52. PMID: 16690755

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ROBO1 (1632-1644) antibody

WB using Anti-ROBO-1 antibody shows detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 µg of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX  for 45 min at room temperature. Rabbit IgG Aff - Purified Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ROBO1 antibody

Immunohistochemistry-Paraffin: Robo1 Antibody [NB600-1254] - 5 ug/ml to detect ROBO1 in a  variety of tissues including multi-human, multi-brain  and multi-cancer slides. This image shows staining  of human brain tissue. Tissue was formalin-fixed  and paraffin embedded. Rabbit IgG Aff - Purified Can, Hu, Ms, Rt C, E, ICC/IF, P, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

ROBO1 antibody

AP23652PU-N DUTT1 antibody  staining of fetal Mouse Brain lysate at 0.05 µg/ml (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Ms, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

ROBO1 antibody

TA303077 (1µg/ml) staining of HUVEC lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ROBO1 Control Peptide

ROBO1 Purified
50 µg / €210.00
  Acris Antibodies GmbH

ROBO1 antibody

Western blot using ROBO1 antibody (AP05287PU-N) on HT-29 cell lysate. Lysate used at 30 µg/lane. Antibody used at 1:400 dilution. Secondary antibody, mouse anti-rabbit HRP, used at 1:50k dilution. Rabbit Aff - Purified Hu WB
10 Blots / €480.00
  Acris Antibodies GmbH

ROBO1 antibody

Western blot using ROBO1 antibody (AP05287SU-N) on human brain lysate. Lysate used at 15 µg/lane. Antibody used at 10 µg/ml. Secondary antibody, mouse anti rabbit HRP, used at 1:50k. Rabbit Purified Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

ROBO1 antibody

Figure 1. Staining ROBO1 in Brain by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ROBO1 (994-1006) antibody

Human Liver (formalin-fixed, paraffin-embedded) stained with ROBO1 antibody AP22501PU-N at 2 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, L, Mky, Ms, Op, Por, Prim, Rt, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ROBO1 antibody

ROBO1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ROBO1 antibody

Detection limit for recombinant GST tagged ROBO1 is approximately 0.03ng/ml as a capture antibody. Mouse IgG2a 2G6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ROBO1 antibody

Detection limit for recombinant GST tagged ROBO1 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 1F8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ROBO1 antibody

ROBO1 polyclonal antibody ( Cat # PAB6930 ) ( 1 µg/ml ) staining of HUVEC lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ROBO1 antibody

ROBO1 Goat Ms, Rt E
0.1 mg / €400.00
  Abnova Taiwan Corp.

ROBO1 antibody

Western blot using ROBO1 polyclonal antibody ( Cat # PAB10401 ) on human brain lysate. Lysate used at 15 µg/lane. Antibody used at 10 µg/ml. Hu
0.1 mg / €400.00
  Abnova Taiwan Corp.

ROBO1 antibody

Immunohistochemistry of ROBO1 polyclonal antibody ( Cat # PAB11258 ) was used at a concentration of 5 µg/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows staining of human brain tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA. Rabbit Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

ROBO1 antibody

ROBO-1 staining of cryostat tissue sections of rat embryo (Neural Tube) at  a concentration of 5 µg/mL using HRP-DAB detection (brown) and counterstained with hematoxylin (blue). Goat Aff - Purified Rt C, E, WB
0.1 mg / €450.00
  Neuromics Antibodies

ROBO1 antibody

Western Blot: ROBO1 Antibody [NB100-41089] - NB100-41089 (1ug/ml) staining of HUVEC lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ROBO1 antibody

ROBO1 Goat IgG Aff - Purified Hu E, WB
0.1 mg / €320.00
  Novus Biologicals Inc.

ROBO1 antibody

Western Blot and Immunoprecipitation: ROBO1 Antibody [NB100-60458] - Detection of Human ROBO1 on HeLa whole cell lysate using NB100-60458. ROBO1 was also immunoprecipitated by rabbit anti-ROBO1 antibody NB100-60459. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

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