ROCK1 antibody

Principal name

ROCK1 antibody

Alternative names for ROCK1 antibody

ROCK-1, p160ROCK, NY-REN-35, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1

SwissProt ID

O77819 (Rabit), P70335 (Mouse), Q13464 (Human), Q63644 (Rat), Q8MIT6 (Bovin)

Gene ID

6093 (ROCK1), 19877 (Rock1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Mky (Monkey), Por (Porcine), Rb (Rabbit), Can (Canine), Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of ROCK1 antibody

The Rho target protein, Rho-associated coiled coil-containing protein kise (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-termil kise domain and an inhibitory cysteine/histidine-rich C-termil domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-termil inhibitory domain is removed, resulting in deregulated and constitutive kise activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ROCK1 antibody

  Rabbit polyclonal antibody to ROCK1 (Rho-associated, coiled-coil containing protein kinase 1)  
ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis. A. 30 ug NIH-3T3 whole cell extract. B. 30 ug C2Cl2 whole cell extract. 5 % SDS-PAGE. ROCK1 antibody [N1N2], N-term (TA308951) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK1 (N-term) antibody

  Rabbit Polyclonal ROCK1 Antibody  
Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ROCK1 antibody

  Anti-ROCK1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human breast and gastric cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ROCK1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK1 antibody

  Anti-ROCK1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ROCK1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK1 antibody

  Rabbit Polyclonal ROCK1 Antibody  
Western blot analysis of ROCK1 expression in Hela whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK1 antibody

  Mouse Monoclonal cleaved ROCK1 Antibody (154C1465)  
Western Blot: cleaved ROCK1 Antibody (154C1465) [TA336423] - Analysis of cleaved ROCK-1 in A) untreated, B) 1 hour and C) 4 hour staurosporin-treated (2 uM) Jurkat cells using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL subst Mouse IgG 154C1465 Purified Can, Hu, Ms, Prim, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ROCK1 antibody

ROCK1 Mouse IgG 12M03 Purified Hu WB
0.1 mg / €380.00
  Acris Antibodies GmbH

ROCK1 antibody

Immunohistochemistry analyzes of Rock-1 antibody (Cat.-No.: AP20652PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Rock1 - mouse gene knockout kit via CRISPR

Rock1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rock1 - mouse gene knockout kit via CRISPR

Rock1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rock1 - mouse gene knockout kit via CRISPR

Rock1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rock1 - mouse gene knockout kit via CRISPR

Rock1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ROCK1 overexpression lysate

ROCK1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Lysates (Knockout)

Catalog No.    

ROCK1-knockout Cell lysate

ROCK1-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ROCK1 (mGFP-tagged) - Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), 200 uL, >10^7 TU/mL

Lenti ORF particles, ROCK1 (mGFP-tagged) - Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), 200 uL, >10^7 TU/mL  
200 µl / €2,530.00
  OriGene Technologies, Inc.

Lenti ORF particles, ROCK1 (Myc-DDK tagged) - Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, ROCK1 (Myc-DDK tagged) - Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1) , 200 uL, >10^7 TU/mL  
200 µl / €2,280.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rock1 (GFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Rock1 (GFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1), 200 uL, >10^7 TU/mL  
200 µl / €2,250.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rock1 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Rock1 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock1), 200 uL, >10^7 TU/mL  
200 µl / €2,030.00
  OriGene Technologies, Inc.

18 Item(s)

per page
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