ROCK1 antibody

Principal name

ROCK1 antibody

Alternative names for ROCK1 antibody

ROCK-1, p160ROCK, NY-REN-35, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1

SwissProt ID

O77819 (Rabit), P70335 (Mouse), Q13464 (Human), Q63644 (Rat), Q8MIT6 (Bovin)

Gene ID

6093 (ROCK1)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Mky (Monkey), Rb (Rabbit), Rt (Rat), Bov (Bovine), Por (Porcine), Prim (Primate)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of ROCK1 antibody

The Rho target protein, Rho-associated coiled coil-containing protein kinase (ROCK)-1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, has recently been shown to contribute significantly to myosin light chain (MLC) activation through two pathways: direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility that is necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain that is located within a pleckstrin-homology region. They are joined by a variable region that contains the Rho-binding domain. During apoptosis, ROCK-I is cleaved by caspase-3 at a conserved DETD1113/G sequence and its carboxy-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3-mediated cleavage and activation of ROCK I induces phosphorylation of MLC and membrane blebbing, one of the first events in the execution phase of apoptosis.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ROCK1 antibody

ROCK1 antibody [N1N2], N-term detects ROCK1 protein by western blot analysis. A. 30 ug NIH-3T3 whole cell extract. B. 30 ug C2Cl2 whole cell extract. 5 % SDS-PAGE. ROCK1 antibody [N1N2], N-term (TA308951) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK1 (N-term) antibody

Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ROCK1 antibody

Predicted cell location: Cytoplasm. Positive control: Human breast and gastric cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ROCK1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK1 antibody

Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ROCK1 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK1 antibody

Western blot analysis of ROCK1 expression in Hela whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK1 antibody

Western Blot: cleaved ROCK1 Antibody (154C1465) [TA336423] - Analysis of cleaved ROCK-1 in A) untreated, B) 1 hour and C) 4 hour staurosporin-treated (2 uM) Jurkat cells using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL subst Mouse IgG 154C1465 Purified Can, Hu, Ms, Prim, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ROCK1 antibody

ROCK1 Mouse IgG 12M03 Purified Hu WB
0.1 mg / €420.00
  Acris Antibodies GmbH

ROCK1 antibody

Immunohistochemistry analyzes of Rock-1 antibody (Cat.-No.: AP20652PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

ROCK1 control peptide

Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
50 µg / €160.00
  Acris Antibodies GmbH

ROCK1 antibody

Immunofluorescence of monoclonal antibody to ROCK1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 2E2 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ROCK1 antibody

ROCK1 Mouse IgG1 2E2 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

ROCK1 antibody

ROCK1 Rabbit Hu, Ms, Rt WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

ROCK1 antibody

Immunofluorescent analysis of ROCK1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Mky, Ms, Por, Rb, Rt ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ROCK1 antibody

Immunofluorescent analysis of ROCK1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Mky, Ms, Por, Rb, Rt ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ROCK1 antibody

Immunofluorescent analysis of ROCK1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Mky, Ms, Por, Rb, Rt ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ROCK1 antibody

WB and IP: ROCK1 Antibody [NB100-624] - Detection of human and mouse Rho Kinase Beta (ROCK1/p160ROCK) on HeLa whole cell lysate using NB100-624. Rho Kinase Beta was also IPed using NB100-622 and NB100-623 at 3 ug/mg lysate. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

ROCK1 antibody

Immunohistochemistry: ROCK1 Antibody (EP786Y) [NB110-57465] - Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma using anti-ROCK1 (cat # 110-57465). Rabbit IgG EP786Y Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €490.00
  Novus Biologicals Inc.

ROCK1 antibody

ROCK1 Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

ROCK1 antibody

ROCK1 Rabbit IgG 12M03 Aff - Purified Hu, Ms WB
0.1 mg / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ROCK1

ROCK1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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