ROCK2 antibody

Principal name

ROCK2 antibody

Alternative names for ROCK2 antibody

ROCK-2, p164ROCK-2, KIAA0619, Rho-associated protein kinase 2, Rho kinase 2, Rho-associated, coiled-coil-containing protein kinase 2

SwissProt ID

O75116 (Human), P70336 (Mouse), Q28021 (Bovin), Q62868 (Rat)

Gene ID

9475 (ROCK2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Can (Canine), Dros (Drosophila), Xen (Xenopus), Frog, Por (Porcine), Sh (Sheep)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Frozen Sections (C)

Background of ROCK2 antibody

Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa.
Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24.1 Thumkeo et al. (2003) concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.2

General readings

Circ Res. 2005 Apr 15;96(7):740-7. Epub 2005 Mar 17.
Functional effects of rho-kinase-dependent phosphorylation of specific sites on cardiac troponin.
Vahebi S, Kobayashi T, Warren CM, de Tombe PP, Solaro RJ.
University of Illinois at Chicago Department of Physiology and Biophysics and Center for Cardiovascular Research College of Medicine, Chicago, Ill 60612-7342, USA.

Items 1 to 20 of 40 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ROCK2 (Center) antibody

Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 ug/mL Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ROCK2 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human cervical and ovarian cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using ROCK2 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK2 antibody

Predicted band size: 161 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK2 antibody

Western blot analysis of ROCK2 expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK2 antibody

Western blot analysis of extracts from Cos7 cells, using ROCK2 (Ab-722) antibody. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ROCK2 antibody

Western blot analysis of extracts of various cell lines, using ROCK2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

ROCK2 (1279-1389) antibody

Human Vessel: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1E12 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

ROCK2 pTyr256 antibody

Western blot using affinity purified anti-ROCK-2 pTyr256 antibody shows detection of phosphorylated ROCK-2 in transfected 293T cells. Lane 1 shows lysate expressing exogenous ROCK-2 where the insert contains the transversion Y256A. Lane 2 shows the lysate expressing WT ROCK-2. A lysate containing a GST null vector also shows no specific staining (data not shown). Primary antibody was used at a 1:1000 dilution. Rabbit IgG Aff - Purified Bov, Can, Chk, Dros, Hu, Ms, Rt, Xen E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

ROCK2 antibody

Human Adrenal (formalin-fixed, paraffin-embedded) stained with ROCK2 antibody AP22644PU-N at 1/100 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Purified Hu, Ms, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

ROCK2 (N-term) antibody

ROCK2 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

ROCK2 control peptide

ROCK2
50 µg / €160.00
  Acris Antibodies GmbH

ROCK2 antibody

Immunofluorescence of monoclonal antibody to ROCK2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2b 2A4 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ROCK2 antibody

Immunoperoxidase of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml] Mouse IgG1 1E12 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ROCK2 antibody

ROCK2 Rabbit Hu, Ms, Rt C, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

ROCK2 antibody

ROCK2 Rabbit Hu, Ms IP, WB
0.1 ml / €400.00
  Abnova Taiwan Corp.

ROCK2 pTyr256 antibody

Western blot using ROCK2 (phospho Y256) polyclonal antibody ( Cat # PAB11303 ) shows detection of phosphorylated ROCK2 in transfected 293T cells.
Lane 1 shows lysate expressing exogenous ROCK2 where the insert contains the transversion Y256A.
Lane 2 shows the lysate expressing WT ROCK2.
The primary antibody was used at a 1 : 1000 dilution.
Personal Communication, A. Papageorge and X. Qian, NCI, Bethesda, MD. Rabbit Bov, Chk, Frog, Hu, Ms, Rt C, E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

ROCK2 antibody

Immunohistochemical staining of ROCK2 on formalin fixed, paraffin embedded human intestinal cancer with ROCK2 polyclonal antibody ( Cat # PAB12328 ). Rabbit Hu, Ms, Rt P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

ROCK2 pThr396 antibody

The cell lysate derived from Conditioned NIH/3T3 cell was immunoprecipitated by ROCK2 ( phospho T396 ) polyclonal antibody ( Cat # PAB12643 ), then immunoprobed by the same antibody at 1 : 500 ( lane 1 ). Lane 2 is a negative control. Rabbit Bov, Chk, Hu, Ms, Rt C, E, IP, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ROCK2 antibody

The cell lysate derived from NIH/3T3 was immunoprecipitated by ROCK2 polyclonal antibody ( Cat # PAB12728 ), then immunoprobed by the same antibody at 1 : 500 ( lane 2 ). Lane 1 is a negative control. Rabbit Bov, Chk, Hu, Ms, Rt C, E, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

ROCK2 antibody

The cell lysate derived from NIH/3T3 was immunoprecipitated by ROCK2 polyclonal antibody ( Cat # PAB12729 ), then immunoprobed by the same antibody at 1 : 500 ( lane 1 ). Lane 2 is a negative control. Rabbit Bov, Chk, Hu, Ms, Rt C, E, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Items 1 to 20 of 40 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn