RPA1 antibody

Principal name

RPA1 antibody

Alternative names for RPA1 antibody

REPA1, RFA1, RF-A protein 1, RPA70, RP-A p70, Replication protein A, Replication protein A 70 kDa DNA-binding subunit, Replication factor A protein 1, Single-stranded DNA-binding protein

SwissProt ID

P22336 (Yeast), P27694 (Human), Q01588 (Xenla), Q19537 (Caeel), Q24492 (Drome), Q5ZJJ2 (Chick), Q6NY74 (Danre), Q8VEE4 (Mouse), Q92372 (Schpo)

Gene ID

6117 (RPA1), 851266 (RFA1), 68275 (Rpa1)

Available reactivities

Hu (Human), S. cerevisiae (Saccharomyces cerevisiae), Ye (Yeast), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ChIP assay (ChIP), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Paraffin Sections (P)

Background of RPA1 antibody

RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded D binding protein, whose D binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular D metabolism such as D replication, recombition, cell cycle and D damage checkpoints, and all major types of D repair including nucleotide excision, base excision, mismatch and double-strand break repairs. In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 checkpoint complex. RPA is hyperphosphorylated upon D damage or replication stress by checkpoint kises including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and D-dependent protein kise (D-PK). Hyperphosphorylation may alter RPA-D and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PC and Pol a, and also proteins involved in SV40 replication, such as D polymerase I and SV40 large T antigen .

General readings

1. Mol Cell. 2009 Oct 23;36(2):193-206.
J Biol Chem. 2009 Dec 11;284(50):34682-91.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RPA1 antibody

  Rabbit Polyclonal antibody to RPA70 (replication protein A1, 70kDa)  
Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308716 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA1 antibody

  Rabbit polyclonal Anti-RPA1 Antibody  
WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

RPA1 antibody

  Mouse Monoclonal RPA70 Antibody  
Western blot detection of RPA70 in Hela,A431,MCF7,COS7,HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa. Mouse IgG2a 8C3-D12-H10 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA1 antibody

Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using RPA1 antibody Cat.-No AM06744PU-N with DAB staining. Mouse IgG1 4C4 Purified Hu, Mky E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

RPA1 (323-337) antibody

  Goat Polyclonal RPA1/RPA70 Antibody  
Anti-RPA1 (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RPA1 antibody

  Anti-RPA1 Rabbit Polyclonal Antibody  
Predicted band size: 68 kDa . Positive control: A431 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA1 antibody

  Rabbit polyclonal Anti-RPA1 Antibody  
WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysateRPA1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells Rabbit IgG Purified Bov, Can, Eq, GP, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

RPA1 - human gene knockout kit via CRISPR

RPA1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RPA1 - human gene knockout kit via CRISPR

RPA1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RPA1 - human gene knockout kit via CRISPR

RPA1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RPA1 - human gene knockout kit via CRISPR

RPA1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rpa1 - mouse gene knockout kit via CRISPR

Rpa1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rpa1 - mouse gene knockout kit via CRISPR

Rpa1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rpa1 - mouse gene knockout kit via CRISPR

Rpa1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rpa1 - mouse gene knockout kit via CRISPR

Rpa1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RPA1

  Recombinant protein of human replication protein A1, 70kDa (RPA1)  
RPA1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

RPA1 overexpression lysate

RPA1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RPA1 (mGFP-tagged) - Human replication protein A1, 70kDa (RPA1), 200 uL, >10^7 TU/mL

Lenti ORF particles, RPA1 (mGFP-tagged) - Human replication protein A1, 70kDa (RPA1), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, RPA1 (Myc-DDK tagged) - Human replication protein A1, 70kDa (RPA1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, RPA1 (Myc-DDK tagged) - Human replication protein A1, 70kDa (RPA1) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rpa1 (GFP-tagged) - Mouse replication protein A1 (Rpa1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Rpa1 (GFP-tagged) - Mouse replication protein A1 (Rpa1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,300.00
  OriGene Technologies, Inc.

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