RPA1 antibody

Principal name

RPA1 antibody

Alternative names for RPA1 antibody

REPA1, RFA1, RF-A protein 1, RPA70, RP-A p70, Replication protein A, Replication protein A 70 kDa DNA-binding subunit, Replication factor A protein 1, Single-stranded DNA-binding protein

SwissProt ID

P22336 (Yeast), P27694 (Human), Q01588 (Xenla), Q19537 (Caeel), Q24492 (Drome), Q5ZJJ2 (Chick), Q6NY74 (Danre), Q8VEE4 (Mouse), Q92372 (Schpo)

Gene ID

6117 (RPA1), 851266 (RFA1), 25885 (POLR1A)

Available reactivities

Hu (Human), S. cerevisiae (Saccharomyces cerevisiae), Ye (Yeast), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), PLA/Proximity ligation assay (PLA), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of RPA1 antibody

RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded D binding protein, whose D binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular D metabolism such as D replication, recombition, cell cycle and D damage checkpoints, and all major types of D repair including nucleotide excision, base excision, mismatch and double-strand break repairs. In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 checkpoint complex. RPA is hyperphosphorylated upon D damage or replication stress by checkpoint kises including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and D-dependent protein kise (D-PK). Hyperphosphorylation may alter RPA-D and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PC and Pol a, and also proteins involved in SV40 replication, such as D polymerase I and SV40 large T antigen .

General readings

1. Mol Cell. 2009 Oct 23;36(2):193-206.
J Biol Chem. 2009 Dec 11;284(50):34682-91.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RPA1 antibody

Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308716 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA1 antibody

WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

RPA1 antibody

Western blot detection of RPA70 in Hela,A431,MCF7,COS7,HT1080 and K562 cell lysates using RPA70 mouse mAb (1:1000 diluted).Predicted band size:70KDa.Observed band size:70KDa. Mouse IgG2a 8C3-D12-H10 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA1 antibody

Immunohistochemical analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using RPA1 antibody Cat.-No AM06744PU-N with DAB staining. Mouse IgG1 4C4 Purified Hu, Mky E, F, ICC/IF, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

RPA1 antibody

Human 721_B; Host:Rabbit. Target Name:RPA1. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. RPA1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; RPA1 antibody - middle region (ARP56526_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

RPA1 (323-337) antibody

Anti-RPA1 (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RPA1 antibody

Predicted band size: 68 kDa . Positive control: A431 cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA1 antibody

WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysateRPA1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells Rabbit IgG Purified Bov, Can, Eq, GP, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

RPA1 antibody

RPA1 polyclonal antibody (A01), Lot # 050727JC01 Western Blot analysis of RPA1 expression in Y-79 ( Cat # L042V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RPA1 antibody

RPA1 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RPA1 antibody

RPA1 Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

RPA1 antibody

RPA1 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RPA1 antibody

RPA1 Mouse IgG1 2H10, 70C or α Hu IP, WB
0.1 mg / €820.00
  Abnova Taiwan Corp.

RPA1 antibody

Human HeLa; WB Suggested Anti-RPA1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Hela cell lysate. RPA1 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells.; RPA1 antibody - middle region (ARP56525_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

RPA1 antibody

Western blot analysis of RPA1 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

RPA1 antibody

Western blot analysis of RPA1 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

RPA1 antibody

Western blot analysis of RPA1 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

RPA1 antibody

Immunohistochemistry-Paraffin: RPA70 Antibody [NB100-2204] - FFPE section of human seminoma.   Affinity purified rabbit anti-RPA70 used at a dilution of 1:250.  Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit Aff - Purified Hu IP, P, PLA, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

RPA1 antibody

Immunohistochemistry: RPA70 Antibody [NBP1-31382] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NBP1-31382 at 1:100 dilution. Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

RPA1 antibody

Immunohistochemistry: RPA70 Antibody (EPR3472) [NBP1-40642] - Immunohistochemical analysis of paraffin-embedded human cervical squamous cell carcinoma tissue using anti-RPA70 Rabbit IgG EPR3472 Hu F, ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

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