RPA2 antibody

Principal name

RPA2 antibody

Alternative names for RPA2 antibody

REPA2, RFA2, RF-A protein 2, RPA32, RP-A p32, RPA34, RP-A p34, Replication protein A, Replication protein A 32 kDa subunit, Replication factor A protein 2, Replication protein A 34 kDa subunit

SwissProt ID

P15927 (Human), P26754 (Yeast), Q62193 (Mouse), Q63528 (Rat), Q92373 (Schpo)

Gene ID

6118 (RPA2), 855404 (RFA2), 59102 (Rpa2)

Available reactivities

Ye (Yeast), Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)

Background of RPA2 antibody

RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded D binding protein, whose D binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular D metabolism such as D replication, recombition, cell cycle and D damage checkpoints, and all major types of D repair including nucleotide excision, base excision, mismatch and double-strand break repairs. In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex. RPA is hyperphosphorylated upon D damage or replication stress by checkpoint kises including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and D-dependent protein kise (D-PK). Hyperphosphorylation may alter RPA-D and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PC and Pol ¦Á, and also proteins involved in SV40 replication, such as D polymerase I and SV40 large T antigen.

General readings

Wang M., et al. (2000) Biochemistry. 39(21):6433-9.
Mer G., et al. (2000) Cell. 103(3):449-56.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763) Mouse IgG1 OTI9A1 Purified Hu, Mky F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Sample (30 ug of whole cell lysate). A: 293T. B: A431 . 12% SDS PAGE. TA308952 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Western blot analysis of extracts of A549 cell lines, using RPA2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500762) Mouse IgG1 OTI10G1 Purified Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500764) Mouse IgG1 OTI3A12 Purified Hu F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765) Mouse IgG1 OTI1H10 Purified Can, Hu, Mky, Rt F, ICC/IF, IP, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786) Mouse IgG2a OTI7C12 Purified Can, Hu, Mky F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788) Mouse IgG1 OTI8B3 Purified Hu F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunoperoxidase of purified MaxPab antibody to RPA2 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml] Mouse Serum Hu E(detection), ICC/IF, P, WB
50 µl / €370.00
  Abnova Taiwan Corp.

RPA2 antibody

Immunoperoxidase of purified MaxPab antibody to RPA2 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RPA2 antibody

Western blot analysis of extracts from K562 cells, using RFA2 (Ab-21) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using RFA2 (Phospho-Thr21) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Western blot analysis of extracts from 3T3 cells, treated with Adriamycin (0.5ug/ml, 24hours), using RFA2 (Phospho-Ser33) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Western blot analysis of RFA2 expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Western blot analysis of RFA2 phosphorylation expression in Adriamycin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Western blot detection of RPA32/RPA2 in MCF7,Hela and HUVEC cell lysates using RPA32/RPA2 mouse mAb (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.Exposure time:20s. Mouse IgG2b 3E7-B5-F2 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Anti-RPA2 mouse monoclonal antibody (TA500766) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715). Mouse IgG1 OTI4C3 Purified Hu ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500812, Dilution 1:50) Mouse IgG1 OTI7F4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemistry analyzes of RFA2 antibody (AP20269PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

RPA2 (N-term) antibody

Western blot analysis of RPA2 (arrow) using rabbit polyclonal RPA2 Antibody (N-term) (Cat. #AP53702PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RPA2 gene. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

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