RPA2 antibody
Principal name
RPA2 antibody
Alternative names for RPA2 antibody
REPA2, RFA2, RF-A protein 2, RPA32, RP-A p32, RPA34, RP-A p34, Replication protein A, Replication protein A 32 kDa subunit, Replication factor A protein 2, Replication protein A 34 kDa subunit
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)
Background of RPA2 antibody
RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded D binding protein, whose D binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular D metabolism such as D replication, recombition, cell cycle and D damage checkpoints, and all major types of D repair including nucleotide excision, base excision, mismatch and double-strand break repairs. In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex. RPA is hyperphosphorylated upon D damage or replication stress by checkpoint kises including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and D-dependent protein kise (D-PK). Hyperphosphorylation may alter RPA-D and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PC and Pol ¦Á, and also proteins involved in SV40 replication, such as D polymerase I and SV40 large T antigen.
General readings
Wang M., et al. (2000) Biochemistry. 39(21):6433-9.
Mer G., et al. (2000) Cell. 103(3):449-56.
Immunoperoxidase of purified MaxPab antibody to RPA2 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to RPA2 on HeLa cell. [antibody concentration 10 ug/ml]
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human placenta.
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human stomach.
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human kidney.
Western Blot analysis of RPA2 expression in transfected 293T cell line (H00006118-T01) by RPA2 MaxPab polyclonal antibody.Lane 1: RPA2 transfected lysate(29.81 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to RPA2 on HeLa cell. [antibody concentration 10 ug/ml]
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human liver.
Western Blot analysis of RPA2 expression in transfected 293T cell line (H00006118-T02) by RPA2 MaxPab polyclonal antibody.Lane 1: RPA2 transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.
Immunoperoxidase of purified MaxPab antibody to RPA2 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to RPA2 on HeLa cell. [antibody concentration 10 ug/ml]
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human placenta.
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human stomach.
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human kidney.
Western Blot analysis of RPA2 expression in transfected 293T cell line (H00006118-T01) by RPA2 MaxPab polyclonal antibody.Lane 1: RPA2 transfected lysate(29.81 KDa).Lane 2: Non-transfected lysate.
Western blotting against RFA2 containing Saccharomyces sp. cell lysates. RFA2 (phospho S122) polyclonal antibody ( Cat # PAB11311 ) at 1.0 µg/ml for 1 hr at RT followed by IRDye 800 Sh anti Rabbit IgG 1 : 5000 1 hr RT.
Western blotting against RFA2 containing cell lysates. Lysates from Saccharomyces sp. :Lane 1, wild type.Lane 2, constitutional unphosphorylated.Lane 3, constitutional phosphorylated.Lane 4 induced phosphorylation.Blot incubated for 1 hr at RT with affinity purified Rabbit anti RFA2 pan reactive at 1.0 µg/ml followed by 1 : 5000 dilution of IRDye800 Sheep anti Rabbit IgG for 1 hr at RT.
Immunohistochemistry analyzes of RFA2 antibody (AP20269PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot analysis of RFA2 antibody (AP20269PU-N) in extracts from HepG2 cells.
Recombinant human RPA2, 1-270 aa, His-tagged
Western blot analysis of RPA2 (arrow) using rabbit polyclonal RPA2 Antibody (N-term) (Cat. #AP53702PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RPA2 gene.
Western blot analysis of RPA2 Antibody (N-term) (Cat. #AP53702PU-N) in mouse testis tissue lysates (35µg/lane). RPA2 (arrow) was detected using the purified Pab.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500762)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500762)
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
HEK293T cells transfected with either RC205715 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPA2 antibody(TA500762), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Anti-RPA2 mouse monoclonal antibody (TA500763) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500763),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody(TA500763),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500764)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500764)
Anti-RPA2 mouse monoclonal antibody (TA500764) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC205715 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPA2 antibody(TA500764), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody(TA500764),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500764),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Anti-RPA2 mouse monoclonal antibody (TA500765) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody(TA500765),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500765),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC205715 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPA2 antibody(TA500765), and then analyzed by flow cytometry.
Anti-RPA2 mouse monoclonal antibody (TA500766) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Anti-RPA2 mouse monoclonal antibody (TA500786) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
Immunofluorescent staining of HepG2 cells using anti-RPA2 mouse monoclonal antibody (TA500786).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC205715 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPA2 antibody(TA500786), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500786),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunofluorescent staining of HepG2 cells using anti-RPA2 mouse monoclonal antibody (TA500788).
Anti-RPA2 mouse monoclonal antibody (TA500788) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500788),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody(TA500788),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500812, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500812, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2 .
Sample (30 ug of whole cell lysate). A: 293T. B: A431 . 12% SDS PAGE. TA308952 diluted at 1:1000.
RPA32 antibody detects RPA2 protein by Western blot analysis. A. 30 ug BCL-1 whole cell lysate/extract. 12 % SDS-PAGE. RPA32 antibody (TA308952) dilution: 1:1000
RPA32 antibody detects RPA2 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. RPA32 antibody (TA308952) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using RPA 32 kDa subunit (TA308952) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 32 kDa subunit (TA308952) antibody at 1:200 dilution.
Western blot analysis of extracts from K562 cells, using RFA2 (Ab-21) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of RFA2 expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of A549 cell lines, using RPA2 antibody.
Immunohistochemistry of paraffin-embedded human stomach cancer using RPA2 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using RPA2 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of U2OS cell using RPA2 antibody. Blue: DAPI for nuclear staining.
Western blot detection of RPA32/RPA2 in MCF7,Hela and HUVEC cell lysates using RPA32/RPA2 mouse mAb (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.Exposure time:20s.
Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using anti-RPA32/RPA2 antibody (dilution 1:200).
Immunoprecipitation analysis of Hela cell lysates using RPA32/RPA2 mouse mAb.
Western blot analysis of RFA2 phosphorylation expression in Adriamycin treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts from 3T3 cells, treated with Adriamycin (0.5ug/ml, 24hours), using RFA2 (Phospho-Ser33) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using RFA2 (Phospho-Thr21) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using RFA2 (Phospho-Thr21) antibody.The picture on the right is treated with the synthesized peptide.
- Quick-Filters:
- Highly Validated (8)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500763 | RPA2 antibody |
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| RPA2 mouse monoclonal antibody, clone OTI9A1 (formerly 9A1) | |||||||
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Mouse | IgG1 | OTI9A1 | Purified | Hu, Mky | F, ICC/IF, IP, P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
 +20 additional images |
Top Selling product! Highly validated antibody!
|
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| TA308952 | RPA2 antibody |
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| Rabbit polyclonal antibody to RPA 32 kDa subunit (replication protein A2, 32kDa) | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€416.00
|
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| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA327326 | RPA2 antibody |
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| Rabbit anti-RPA2 Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA500762 | RPA2 antibody |
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| RPA2 mouse monoclonal antibody, clone OTI10G1 (formerly 10G1) | |||||||
 |
Mouse | IgG1 | OTI10G1 | Purified | Hu | F, IP, P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA500764 | RPA2 antibody |
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| RPA2 mouse monoclonal antibody, clone OTI3A12 (formerly 3A12) | |||||||
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Mouse | IgG1 | OTI3A12 | Purified | Hu | F, ICC/IF, IP, P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| TA500765 | RPA2 antibody |
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| RPA2 mouse monoclonal antibody, clone OTI1H10 (formerly 1H10) | |||||||
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Mouse | IgG1 | OTI1H10 | Purified | Can, Hu, Mky, Rt | F, ICC/IF, IP, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +15 additional images |
Highly validated antibody!
|
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| TA500786 | RPA2 antibody |
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| RPA2 mouse monoclonal antibody, clone OTI7C12 (formerly 7C12) | |||||||
 |
Mouse | IgG2a | OTI7C12 | Purified | Can, Hu, Mky | F, ICC/IF, IP, P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +15 additional images |
Highly validated antibody!
|
||||||
| TA500788 | RPA2 antibody |
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| RPA2 mouse monoclonal antibody, clone OTI8B3 (formerly 8B3) | |||||||
 |
Mouse | IgG1 | OTI8B3 | Purified | Hu | F, ICC/IF, IP, P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
||||||
| TA312065 | RPA2 antibody |
||||||
| Rabbit polyclonal RFA2 (Ab-21) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA312066 | RPA2 antibody |
||||||
| Rabbit polyclonal RFA2 (Thr21) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA312067 | RPA2 antibody |
||||||
| Rabbit polyclonal RFA2 (Ser33) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325816 | RPA2 antibody |
||||||
| Rabbit Polyclonal RFA2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325817 | RPA2 antibody |
||||||
| Rabbit Polyclonal RFA2 (Thr21) Antibody (Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347041 | RPA2 antibody |
||||||
| Mouse Monoclonal RPA32/RPA2 Antibody | |||||||
 |
Mouse | IgG2b | 3E7-B5-F2 | Purified | ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA500766 | RPA2 antibody |
||||||
| RPA2 mouse monoclonal antibody, clone OTI4C3 (formerly 4C3) | |||||||
 |
Mouse | IgG1 | OTI4C3 | Purified | Hu | ICC/IF, IP, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA500812 | RPA2 antibody |
||||||
| anti-RPA2 mouse monoclonal antibody, clone OTI7F4 (formerly 7F4) | |||||||
 |
Mouse | IgG1 | OTI7F4 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| AP20269PU-N | RPA2 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 mg /
€350.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP53702PU-N | RPA2 (N-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€370.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| R1568P | RPA2 antibody |
||||||
 |
Rabbit | Aff - Purified | Ye | E, WB |
0.1 mg /
€460.00
|
||
| OriGene Technologies GmbH | |||||||
| R1571P | RPA2 pSer122 antibody |
||||||
|
Rabbit | Aff - Purified | Ye | E, WB |
0.1 mg /
€460.00
|
||
| OriGene Technologies GmbH | |||||||
