RPA2 antibody

Principal name

RPA2 antibody

Alternative names for RPA2 antibody

REPA2, RFA2, RF-A protein 2, RPA32, RP-A p32, RPA34, RP-A p34, Replication protein A, Replication protein A 32 kDa subunit, Replication factor A protein 2, Replication protein A 34 kDa subunit

SwissProt ID

P15927 (Human), P26754 (Yeast), Q62193 (Mouse), Q63528 (Rat), Q92373 (Schpo)

Gene ID

6118 (RPA2), 855404 (RFA2), 84172 (POLR1B)

Available reactivities

Hu (Human), Ms (Mouse), Ye (Yeast), Rt (Rat), Hst (Hamster), S. cerevisiae (Saccharomyces cerevisiae), Can (Canine), Mky (Monkey)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of RPA2 antibody

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

General readings

Niu H et al. Mapping of amino acid residues in the p34 subunit of human single-stranded DNA-binding protein phosphorylated by DNA-dependent protein kinase and Cdc2 kinase in vitro. J Biol Chem 272:12634-41 (1997). PubMed PMID: 9139719
Pan ZQ et al. Phosphorylated and unphosphorylated forms of human single-stranded DNA-binding protein are equally active in simian virus 40 DNA replication and in nucleotide excision repair. Proc Natl Acad Sci U S A 92:4636-40 (1995). PubMed PMID: 7753855
Kenny MK et al. The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication. J Biol Chem 265:7693-700 (1990). PubMed PMID: 2159011

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763) Mouse IgG1 OTI9A1 Purified Hu, Mky F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Sample (30 ug of whole cell lysate). A: 293T. B: A431 . 12% SDS PAGE. TA308952 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Western blot analysis of extracts of A549 cell lines, using RPA2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500762) Mouse IgG1 OTI10G1 Purified Hu F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500764) Mouse IgG1 OTI3A12 Purified Hu F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765) Mouse IgG1 OTI1H10 Purified Can, Hu, Mky, Rt F, ICC/IF, IP, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786) Mouse IgG2a OTI7C12 Purified Can, Hu, Mky F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788) Mouse IgG1 OTI8B3 Purified Hu F, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunoperoxidase of purified MaxPab antibody to RPA2 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml] Mouse Serum Hu E(detection), ICC/IF, P, WB
50 µl / €370.00
  Abnova Taiwan Corp.

RPA2 antibody

Immunoperoxidase of purified MaxPab antibody to RPA2 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RPA2 antibody

Western blot analysis of extracts from K562 cells, using RFA2 (Ab-21) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Western blot analysis of RFA2 expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Western blot detection of RPA32/RPA2 in MCF7,Hela and HUVEC cell lysates using RPA32/RPA2 mouse mAb (1:2000 diluted).Predicted band size:32KDa.Observed band size:32KDa.Exposure time:20s. Mouse IgG2b 3E7-B5-F2 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPA2 antibody

Anti-RPA2 mouse monoclonal antibody (TA500766) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715). Mouse IgG1 OTI4C3 Purified Hu ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500812, Dilution 1:50) Mouse IgG1 OTI7F4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RPA2 antibody

Immunohistochemistry analyzes of RFA2 antibody (AP20269PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

RPA2 (N-term) antibody

Western blot analysis of RPA2 (arrow) using rabbit polyclonal RPA2 Antibody (N-term) (Cat. #AP53702PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RPA2 gene. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RPA2 antibody

RPA2 Rabbit Aff - Purified Ye E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

RPA2 pSer122 antibody

RPA2 Rabbit Aff - Purified Ye E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

RPA2 antibody

Immunofluorescence of purified MaxPab antibody to RPA2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

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