RPA2 antibody
Principal name
RPA2 antibody
Alternative names for RPA2 antibody
REPA2, RFA2, RF-A protein 2, RPA32, RP-A p32, RPA34, RP-A p34, Replication protein A, Replication protein A 32 kDa subunit, Replication factor A protein 2, Replication protein A 34 kDa subunit
Ncbi ID
NP_002937.1, 9606, NP_002937, 4932, NP_014087, NP_014087.1, NM_002946
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), PLA/Proximity ligation assay (PLA), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F)
Background of RPA2 antibody
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.
General readings
Niu H et al. Mapping of amino acid residues in the p34 subunit of human single-stranded DNA-binding protein phosphorylated by DNA-dependent protein kinase and Cdc2 kinase in vitro. J Biol Chem 272:12634-41 (1997). PubMed PMID: 9139719
Pan ZQ et al. Phosphorylated and unphosphorylated forms of human single-stranded DNA-binding protein are equally active in simian virus 40 DNA replication and in nucleotide excision repair. Proc Natl Acad Sci U S A 92:4636-40 (1995). PubMed PMID: 7753855
Kenny MK et al. The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication. J Biol Chem 265:7693-700 (1990). PubMed PMID: 2159011
Detection of human RPA32 protein using GeneTex RPA32 mouse polyclonal antiserum (GTX70242) shown in lane 1 and GeneTex RPA32 rabbit polyclonal antiserum (GTX70258) shown in lane 2. Western Blot was done using HeLa whole cell extract.
RPA32 antibody detects RPA32 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RPA32 protein stained by RPA32 antibody (GTX70242) diluted at 1:500.Blue: Hoechst 33342 staining.
RPA32 antibody detects RPA32 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12 % SDS-PAGE, and blotted with RPA32 antibody (GTX70243) diluted by 1:500
Detection of human RPA32 protein using GeneTex RPA32 12F3.3 monoclonal antibody (GTX70243) in HeLa (IR treated) whole cell extract. No radiation, 1hr, 2hr, and 4hr exposure corresponding to lanes 1 through 4 respectively.
RPA32 antibody [12F3.3] detects RPA32 protein at DNA damage foci by immunofluorescent analysis. Sample: 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody [12F3.3] (GTX70243) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
RPA32 antibody detects RPA32 protein at DNA damage foci by immunofluorescent analysis. Sample: 2mM Hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody (GTX70258) diluted at 1:2000. Blue: Hoechst 33342 staining.
GTXat a dilution of 1:2500 staining RPA 32 in A) HeLa whole cell lysate, B) Raji whole cell lysate and C) NIH-3T3 whole cell lysate by Western Blot (ECL)
Detection of human RPA32 protein using GeneTex RPA32 polyclonal antiserum (GTX70258) in HeLa whole cell extract.
Western Blot: RPA2 Antibody 12F3.3 [NB 100-158]-Detection of human RPA32 protein using in HeLa (IR treated) whole cell extract. No radiation, 1hr, 2hr, and 4hr exposure corresponding to lanes 1 through 4, respectively.
Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332] - See Notes, IHC Caption C
Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332] - See Notes, IHC Caption B
Immunohistochemistry-Paraffin: RPA2 Antibody [NB100-332] - See Notes, IHC Caption A
Western Blot:RPA2 Antibody [NB100-347]- Detection of human RPA32 protein using RPA32 polyclonal antiserum in HeLa whole cell extract.
Western Blot: RPA2 Antibody[ NB100-347]- at a dilution of 1:2500 staining RPA 32 in A) HeLa whole cell lysate, B) Raji whole cell lysate and C) NIH-3T3 whole cell lysate by Western Blot (ECL)
Immunohistochemistry-Paraffin: RPA2 [p Ser33] Antibody [NB100-544] - See Notes, IHC Caption
Immunofluorescence:RPA2 [phospho Ser33] Antibody [NB100-544]- Localization of phosphorylated RPA32 to foci following treatment with CPT. Samples: Asynchronous U2OS cells treated with CPT (1 uM, 1 hr). Antibody: NB 100-544 used at 2 mcg/ml. Detection: FITC conjugated anti-rabbit IgG.
Western Blot: RPA32 Phosphospecific Antibody [NB100-544] - Detection of Phosphorylated RPA32 by Western Blot. Samples: Whole cell lysate (20 ug) from asynchronously growing HeLa cells either untreated or treated with 2 or 10 J/m2 of UV irradiation. Antibodies: NB100-544 used at 1 ug/ml (upper panel) or rabbit anti-RPA32 antibody (NB100-332) used at 1 ug/ml (lower panel). Detection: Chemiluminescence.
Immunohistochemistry: RPA2 Antibody (9H8) [NB600-565]- Formalin fixed paraffin embedded human tonsil stained with RPA using ABC method.
Immunohistochemical of paraffin-embedded human gliomas using 10412-1-Ap(RPA2 antibody) at dilution of 1:100 (under 25x lens)
HeLa cells were subjected to SDS PAGE followed by western blot with 10412-1-Ap(RPA2 antibody) at dilution of 1:400
A549 cells were subjected to SDS PAGE followed by western blot with 10412-1-Ap(RPA2 antibody) at dilution of 1:1000
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10412-1-Ap(RPA2 antibody) at dilution of 1:1000
HeLa cells were subjected to SDS PAGE followed by western blot with 10412-1-Ap(RPA2 antibody) at dilution of 1:500
HeLa cells were subjected to SDS PAGE followed by western blot with 10412-1-Ap(RPA2 antibody) at dilution of 1:400
Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10412-1-Ap( RPA2 Antibody) at dilution of 1:1000
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10412-1-Ap(RPA2 antibody) at dilution of 1:1000
Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10412-1-Ap( RPA2 Antibody) at dilution of 1:1000
IP Result of anti-RPA2 (IP:10412-1-Ap, 4ug; Detection:10412-1-Ap 1:1000) with mouse kidney tissue lysate 4000ug.
Immunocytochemical staining of methanol fixed cells with GTX16850.
IHC-P analysis of human tonsil tissue using RPA32 antibody [9H8].
IHC - RPA34 antibody [9H8] RPA34 antibody [9H8]. GTX22175 - immunohistochemistry.
Immunoperoxidase of purified MaxPab antibody to RPA2 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to RPA2 on HeLa cell. [antibody concentration 10 ug/ml]
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human placenta.
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human stomach.
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human kidney.
Western Blot analysis of RPA2 expression in transfected 293T cell line (H00006118-T01) by RPA2 MaxPab polyclonal antibody.Lane 1: RPA2 transfected lysate(29.81 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to RPA2 on HeLa cell. [antibody concentration 10 ug/ml]
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human liver.
Western Blot analysis of RPA2 expression in transfected 293T cell line (H00006118-T02) by RPA2 MaxPab polyclonal antibody.Lane 1: RPA2 transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.
Western Blot: RPA2 Antibody (MA34) [NB100-74526] - Figure 1 shows a Western blot of RPA p34 in purified RPA protein using NB100-74526.
Immunoperoxidase of purified MaxPab antibody to RPA2 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to RPA2 on HeLa cell. [antibody concentration 10 ug/ml]
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human placenta.
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human stomach.
RPA2 MaxPab polyclonal antibody. Western Blot analysis of RPA2 expression in human kidney.
Western Blot analysis of RPA2 expression in transfected 293T cell line (H00006118-T01) by RPA2 MaxPab polyclonal antibody.Lane 1: RPA2 transfected lysate(29.81 KDa).Lane 2: Non-transfected lysate.
Western blotting against RFA2 containing Saccharomyces sp. cell lysates. RFA2 (phospho S122) polyclonal antibody ( Cat # PAB11311 ) at 1.0 µg/ml for 1 hr at RT followed by IRDye 800 Sh anti Rabbit IgG 1 : 5000 1 hr RT.
Western blotting against RFA2 containing cell lysates. Lysates from Saccharomyces sp. :Lane 1, wild type.Lane 2, constitutional unphosphorylated.Lane 3, constitutional phosphorylated.Lane 4 induced phosphorylation.Blot incubated for 1 hr at RT with affinity purified Rabbit anti RFA2 pan reactive at 1.0 µg/ml followed by 1 : 5000 dilution of IRDye800 Sheep anti Rabbit IgG for 1 hr at RT.
Western Blot: RPA2 Lysate [NBL1-15489] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for RPA2
Immunohistochemistry: RFA2 Antibody [NBP1-19430] - RFA2 Antibody [NBP1-19430] - paraffin-embedded human lung carcinoma tissue.
Western Blot: RFA2 Antibody [NBP1-19430] - extracts from HepG2 cells.
Immunohistochemistry: Phospho-RPA32 Antibody(S4/S8)[NBP1-23017]- FFPE serial sections of mouse colon carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) used at a dilution of 1:250.
Western Blot: RPA2 [p Ser4/Ser8] Antibody [NBP1-23017]-Whole cell lysate 50 mcg for WB in A; Phosphorylated RPA32 was detected using rabbit anti-phospho RPA32 Ser4/Ser8 antibody.
Western Blot: RPA2 [p Ser4, p Ser8] Antibody [NBP1-23017] - Samples: Whole cell lysate (50 ug for WB in A; 1 mg for IP, 20% of IP loaded in B) from HeLa Cells that had been treated with etoposide (+) or mock treated (-). In B, immunoprecipitated RPA32 was either _x000d_treated with phosphatase (+) or mock treated (-). Antibodies: Phosphorylated RPA32 was detected using rabbit anti-phospho RPA32 Ser4/Ser8 antibody NBP1-23017 (lot A300-245A-2) at 1 ug/ml. Total RPA32 was detected using rabbit anti-RPA32 antibody NB100-332 at 1 mcg/ml. RPA32 was immunoprecipitated using NB100-332 at 3 ug/mg lysate.
Immunohistochemistry: Phospho-RPA32 Antibody(S4/S8)[NBP1-23017]- FFPE serial sections of human breast carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) used at a dilution of 1:250
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using RPA32/RPA2(GTX101765) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 12% SDS PAGE GTX101765 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using RPA 32 kDa subunit (GTX113004) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 32 kDa subunit (GTX113004) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) 12% SDS PAGE GTX113004 diluted at 1:1000
RPA32 antibody detects RPA2 protein by Western blot analysis.A. 30 µg PC-12 whole cell lysate/extractB. 30 µg Rat2 whole cell lysate/extract12 % SDS-PAGERPA32 antibody (GTX113004) dilution: 1:1000
RPA32 antibody detects RPA2 protein by Western blot analysis.A. 30 µg BCL-1 whole cell lysate/extract12 % SDS-PAGERPA32 antibody (GTX113004) dilution: 1:1000
Immunohistochemistry: RPA2 Antibody (EPR2877Y) [NBP1-40544] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-RPA32
Western Blot: RPA2 Antibody (EPR2877Y) [NBP1-40544] - Western blot analysis on (A) HeLa, (B) HUVEC, (C) NIH/3T3 and (D) C6 cell lysates using anti-RPA32, dilution 1:10,000.
Immunohistochemistry analyzes of RFA2 antibody (AP20269PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot analysis of RFA2 antibody (AP20269PU-N) in extracts from HepG2 cells.
Recombinant human RPA2, 1-270 aa, His-tagged
Western blot analysis of RPA2 (arrow) using rabbit polyclonal RPA2 Antibody (N-term) (Cat. #AP53702PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RPA2 gene.
Western blot analysis of RPA2 Antibody (N-term) (Cat. #AP53702PU-N) in mouse testis tissue lysates (35µg/lane). RPA2 (arrow) was detected using the purified Pab.
This product was assayed by western blotting against RFA2 containing cell lysates. Blot incubated for 1 hr at RT with affinity purified Rabbit anti-RFA2 pan reactive Lot 13229 at 1.0 µg/ml followed by 1:5000 dilution of IRDye800 sheep anti-Rabbit IgG for 1 hr at RT.
This product was assayed by western blotting against RFA2 containing cell lysates. Affinity purified rabbit anti-RFA2 pS122 specific Lot 13230 at 1.0 µg/ml for 1 hr at RT followed by IRDye 800 sheep anti-Rabbit IgG1:5000 1 hr RT.
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Phospho-RPA32 (pT21) RabMAb (cat. #GTX62664).
Western blot analysis on HeLa cell lysates using anti-Phospho-RPA32 (pT21) RabMAb (cat. #GTX62664). Cells were either (A) untreated (B) treated with Camptothecin.
Immunohistochemical staining of paraffin-embedded colon tissue using anti-RPA2 mouse monoclonal antibody. (GTX83688, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-RPA2 mouse monoclonal antibody. (GTX83688, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-RPA2 mouse monoclonal antibody. (GTX83688, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-RPA2 mouse monoclonal antibody. (GTX83688, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-RPA2 mouse monoclonal antibody. (GTX83688, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded breast tissue using anti-RPA2 mouse monoclonal antibody. (GTX83688, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue using anti-RPA2 mouse monoclonal antibody. (GTX83688, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded bladder tissue using anti-RPA2 mouse monoclonal antibody. (GTX83688, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-RPA2 mouse monoclonal antibody. (GTX83688, Dilution 1:50)
Anti-RPA2 mouse monoclonal antibody (GTX83688) immunofluorescent staining of COS7 cells transiently transfected with RPA2
Immunofluorescent staining of HepG2 cells using anti-RPA2 mouse monoclonal antibody (GTX83688).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2 .
Flow cytometric analysis of Hela cells, using anti-RPA2 antibody(GTX83688),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Flow cytometric analysis of Jurkat cells, using anti-RPA2 antibody(GTX83688),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Immunohistochemical staining of paraffin-embedded liver tissue using anti-RPA2 mouse monoclonal antibody. (GTX83689, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2 .
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-RPA2 mouse monoclonal antibody. (GTX83689, Dilution 1:50)
Anti-RPA2 mouse monoclonal antibody (GTX83690) immunofluorescent staining of COS7 cells transiently transfected with RPA2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-RPA2 mouse monoclonal antibody. (GTX83691, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-RPA2 mouse monoclonal antibody. (GTX83691, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RPA2 mouse monoclonal antibody. (GTX83691, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-RPA2 mouse monoclonal antibody. (GTX83691, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-RPA2 mouse monoclonal antibody. (GTX83691, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded breast tissue using anti-RPA2 mouse monoclonal antibody. (GTX83691, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue using anti-RPA2 mouse monoclonal antibody. (GTX83691, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-RPA2 mouse monoclonal antibody. (GTX83691, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-RPA2 mouse monoclonal antibody. (GTX83691, Dilution 1:50)
Anti-RPA2 mouse monoclonal antibody (GTX83691) immunofluorescent staining of COS7 cells transiently transfected with RPA2
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RPA2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2 .
HEK293T cells transfected with either pCMV6-ENTRY RPA2
Flow cytometric analysis of Hela cells, using anti-RPA2 antibody(GTX83691),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Flow cytometric analysis of Jurkat cells, using anti-RPA2 antibody(GTX83691),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Immunohistochemical staining of paraffin-embedded colon tissue using anti-RPA2 mouse monoclonal antibody. (GTX83692, Dilution 1:50)
Anti-RPA2 mouse monoclonal antibody (GTX83692) immunofluorescent staining of COS7 cells transiently transfected with RPA2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2 .
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RPA2 monoclonal antibody.
Flow cytometric analysis of Jurkat cells, using anti-RPA2 antibody(GTX83692),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Immunohistochemical staining of paraffin-embedded liver tissue using anti-RPA2 mouse monoclonal antibody. (GTX83692, Dilution 1:50)
Flow cytometric analysis of Hela cells, using anti-RPA2 antibody(GTX83692),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
HEK293T cells transfected with either pCMV6-ENTRY RPA2
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded bladder tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded lung tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded thyroid tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RPA2 mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Anti-RPA2 mouse monoclonal antibody (GTX83693) immunofluorescent staining of COS7 cells transiently transfected with RPA2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RPA2 monoclonal antibody.
Flow cytometric analysis of Hela cells, using anti-RPA2 antibody(GTX83693),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-RPA2mouse monoclonal antibody. (GTX83693, Dilution 1:50)
Flow cytometric analysis of Jurkat cells, using anti-RPA2 antibody(GTX83693),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-RPA2 mouse monoclonal antibody. (GTX83694, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2 .
HEK293T cells transfected with either pCMV6-ENTRY RPA2
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Immunohistochemical staining of paraffin-embedded liver tissue using anti-RPA2 mouse monoclonal antibody. (GTX83694, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded breast tissue using anti-RPA2 mouse monoclonal antibody. (GTX83695, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue using anti-RPA2 mouse monoclonal antibody. (GTX83695, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-RPA2 mouse monoclonal antibody. (GTX83695, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-RPA2 mouse monoclonal antibody. (GTX83695, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-RPA2 mouse monoclonal antibody. (GTX83695, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-RPA2 mouse monoclonal antibody. (GTX83695, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-RPA2 mouse monoclonal antibody. (GTX83695, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-RPA2 mouse monoclonal antibody. (GTX83695, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-RPA2 mouse monoclonal antibody. (GTX83695, Dilution 1:50)
Immunofluorescent staining of HepG2 cells using anti-RPA2 mouse monoclonal antibody (GTX83695).
Anti-RPA2 mouse monoclonal antibody (GTX83695) immunofluorescent staining of COS7 cells transiently transfected with RPA2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2 .
HEK293T cells transfected with either pCMV6-ENTRY RPA2
Flow cytometric analysis of Hela cells, using anti-RPA2 antibody(GTX83695),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RPA2 monoclonal antibody.
Immunocytochemistry Analysis:
Representative lot data.
Confocal fluorescent analysis of HeLa and A431 cells using Cat. No. AM50442PU-N, Anti-Replication Protein A, clone RPA34-20 and Donkey Anti-Mouse IgG secondary antibody conjugated to Cy3 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye - Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Representative lot data.
Confocal fluorescent analysis of HeLa and A431 cells using Cat. No. AM50442PU-N, Anti-Replication Protein A, clone RPA34-20 and Donkey Anti-Mouse IgG secondary antibody conjugated to Cy3 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye - Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunohistochemistry Analysis:
Reprsentative lot data.
Formalin Fixed Paraffin Embedded (FFPE) human colorectal adenocarcinoma tissues (Fig. 1 and Fig. 2) were probed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:5 dilution of Cat. No. AM50442PU-N, Anti-Replication Protein A, clone RPA34-20. Reactivity was detected using an Anti-Mouse secondary antibody and HRP-DAB. Positive nuclear staining was observed in human colorectal adenocarcinoma tissues (Fig. 2 illustrates the use of a negative control).
Reprsentative lot data.
Formalin Fixed Paraffin Embedded (FFPE) human colorectal adenocarcinoma tissues (Fig. 1 and Fig. 2) were probed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:5 dilution of Cat. No. AM50442PU-N, Anti-Replication Protein A, clone RPA34-20. Reactivity was detected using an Anti-Mouse secondary antibody and HRP-DAB. Positive nuclear staining was observed in human colorectal adenocarcinoma tissues (Fig. 2 illustrates the use of a negative control).
Western Blotting Analysis:
Representative lot data.
HeLa cell lysate was probed with Anti-Replication Protein A, clone RPA34-20 (1 µg/mL).
Proteins were visualized using a Goat Anti-Mouse IgG (H&L) conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Replication Protein A (~30 kDa).
Representative lot data.
HeLa cell lysate was probed with Anti-Replication Protein A, clone RPA34-20 (1 µg/mL).
Proteins were visualized using a Goat Anti-Mouse IgG (H&L) conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Replication Protein A (~30 kDa).
Immunocytochemistry Analysis:
Representative lot data.
Confocal fluorescent analysis of A431 cells using a 1:250 dilution of, Anti-Replication Protein A, clone RPA34-19 and a Donkey Anti-Mouse IgG secondary antibody conjugated to Cy3 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye - Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Representative lot data.
Confocal fluorescent analysis of A431 cells using a 1:250 dilution of, Anti-Replication Protein A, clone RPA34-19 and a Donkey Anti-Mouse IgG secondary antibody conjugated to Cy3 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye - Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.
Immunohistochemistry Analysis:
Representative lot data.
Formalin Fixed Paraffin Embedded (FFPE) human placental chorionic villi (Fig. 1 and Fig. 2) and human colorectal adenocarcinoma tissues (Fig. 3 and Fig. 4) were probed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:5 dilution of Cat. No. AM50443PU-N, Anti-Replication Protein A, clone RPA34-19. Reactivity was detected using an Anti-Mouse IgG secondary antibody and HRP-DAB. Positive nuclear staining was observed in cytotrophoblastic cells of human placental chorionic villi and in human colorectal adenocarcinoma tissues (Fig.2 and Fig.4 illustrate the use of a negative control.).
Representative lot data.
Formalin Fixed Paraffin Embedded (FFPE) human placental chorionic villi (Fig. 1 and Fig. 2) and human colorectal adenocarcinoma tissues (Fig. 3 and Fig. 4) were probed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:5 dilution of Cat. No. AM50443PU-N, Anti-Replication Protein A, clone RPA34-19. Reactivity was detected using an Anti-Mouse IgG secondary antibody and HRP-DAB. Positive nuclear staining was observed in cytotrophoblastic cells of human placental chorionic villi and in human colorectal adenocarcinoma tissues (Fig.2 and Fig.4 illustrate the use of a negative control.).
Western Blotting Analysis:
Representative lot data.
HeLa cell lysate was probed with Anti-Replication Protein A, clone RPA34-19 (1:2,000 dilution).
Proteins were visualized using a Goat-Anti Mouse IgG (H&L) secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Replication Protein A (~34 kDa).
Representative lot data.
HeLa cell lysate was probed with Anti-Replication Protein A, clone RPA34-19 (1:2,000 dilution).
Proteins were visualized using a Goat-Anti Mouse IgG (H&L) secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates Replication Protein A (~34 kDa).
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500762)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500762)
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
HEK293T cells transfected with either RC205715 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPA2 antibody(TA500762), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500763)
Anti-RPA2 mouse monoclonal antibody (TA500763) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500763),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody(TA500763),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500764)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500764)
Anti-RPA2 mouse monoclonal antibody (TA500764) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells transfected with either RC205715 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPA2 antibody(TA500764), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody(TA500764),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500764),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500765)
Anti-RPA2 mouse monoclonal antibody (TA500765) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody(TA500765),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500765),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC205715 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPA2 antibody(TA500765), and then analyzed by flow cytometry.
Anti-RPA2 mouse monoclonal antibody (TA500766) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500786)
Anti-RPA2 mouse monoclonal antibody (TA500786) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
Immunofluorescent staining of HepG2 cells using anti-RPA2 mouse monoclonal antibody (TA500786).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-RPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC205715 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RPA2 antibody(TA500786), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500786),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500788)
Immunofluorescent staining of HepG2 cells using anti-RPA2 mouse monoclonal antibody (TA500788).
Anti-RPA2 mouse monoclonal antibody (TA500788) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500788),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody(TA500788),(Red), compared to a nonspecific negative control antibody,(Blue).
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500812, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-RPA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500812, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2 .
- Quick-Filters:
- Highly Validated (10)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500763 | RPA2 antibody |
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Mouse | IgG1 | OTI9A1 | Purified | Hu, Mky | F, ICC/IF, IP, P, WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
 +20 additional images |
Top Selling product! Highly validated antibody!
|
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| TA308952 | RPA2 antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA327326 | RPA2 antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA500762 | RPA2 antibody |
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 |
Mouse | IgG1 | OTI10G1 | Purified | Hu | F, IP, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA500764 | RPA2 antibody |
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 |
Mouse | IgG1 | OTI3A12 | Purified | Hu | F, ICC/IF, IP, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +8 additional images |
Highly validated antibody!
|
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| TA500765 | RPA2 antibody |
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 |
Mouse | IgG1 | OTI1H10 | Purified | Can, Hu, Mky, Rt | F, ICC/IF, IP, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +15 additional images |
Highly validated antibody!
|
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| TA500786 | RPA2 antibody |
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 |
Mouse | IgG2a | OTI7C12 | Purified | Can, Hu, Mky | F, ICC/IF, IP, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +15 additional images |
Highly validated antibody!
|
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| TA500788 | RPA2 antibody |
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 |
Mouse | IgG1 | OTI8B3 | Purified | Hu | F, ICC/IF, IP, P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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| H00006118-B01 | RPA2 antibody |
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![Immunoperoxidase of purified MaxPab antibody to RPA2 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00006118-B01-3-8-1-L.jpg) |
Mouse | Serum | Hu | E(detection), ICC/IF, P, WB |
50 µl /
€370.00
|
||
| Abnova Taiwan Corp. | |||||||
| +5 additional images |
Highly validated antibody! |
||||||
| H00006118-B01P | RPA2 antibody |
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![Immunoperoxidase of purified MaxPab antibody to RPA2 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00006118-B01P-3-8-1-L.jpg) |
Mouse | Purified | Hu | E(detection), ICC/IF, P, WB |
50 µg /
€370.00
|
||
| Abnova Taiwan Corp. | |||||||
| +5 additional images |
Highly validated antibody! |
||||||
| TA312065 | RPA2 antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325816 | RPA2 antibody |
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 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347041 | RPA2 antibody |
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 |
Mouse | IgG2b | 3E7-B5-F2 | Purified | ICC/IF, WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA500766 | RPA2 antibody |
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 |
Mouse | IgG1 | OTI4C3 | Purified | Hu | ICC/IF, IP, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA500812 | RPA2 antibody |
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 |
Mouse | IgG1 | OTI7F4 | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP20269PU-N | RPA2 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms | ICC/IF, P, WB |
0.1 mg /
€350.00
|
||
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AP53702PU-N | RPA2 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| R1568P | RPA2 antibody |
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 |
Rabbit | Aff - Purified | Ye | E, WB |
0.1 mg /
€440.00
|
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| Acris Antibodies GmbH | |||||||
| R1571P | RPA2 pSer122 antibody |
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|
Rabbit | Aff - Purified | Ye | E, WB |
0.1 mg /
€440.00
|
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| Acris Antibodies GmbH | |||||||
| H00006118-B02 | RPA2 antibody |
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![Immunofluorescence of purified MaxPab antibody to RPA2 on HeLa cell. [antibody concentration 10 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00006118-B02-4-1-1-L.jpg) |
Mouse | Serum | Hu | E(detection), ICC/IF, WB |
50 µl /
€370.00
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| Abnova Taiwan Corp. | |||||||
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