RPE65 antibody

Principal name

RPE65 antibody

Alternative names for RPE65 antibody

Retinol isomerase, Retinoid isomerohydrolase, All-trans-retinyl-palmitate hydrolase, Retinal pigment epithelium-specific 65 kDa protein

SwissProt ID

O70276 (Rat), Q16518 (Human), Q28175 (Bovin), Q91ZQ5 (Mouse), Q9TVB8 (Canfa), Q9YGX2 (Chick)

Gene ID

6121 (RPE65), 282043, 19892 (Rpe65), 89826

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Can (Canine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)

Background of RPE65 antibody

The visual cycle consisting of retinoids, the enzymatic pathway that produces chromophore after light absorption, is located primarily in the retinal pigment epithelium (RPE) and is essential for rod photoreceptor survival. This visual cycle is naturally disrupted in humans with Leber congenital amaurosis (LCA) which primarily caused by mutations in a retinal epithelial specific 6kDa protein (RPE65). The RPE65 is expressed exclusively in central retinal pigment epithelium and appeared to be concentrated in the central retina. The central retinal RPE layer also showed a 4-fold higher retinoid isomerase activity than more peripheral RPE (1). RPE65 is a isomerhydrolase catalyzing the key step in regeneration of 11-cis-retinal, a chromophore for retinal pigment. The regeneration of retinal pigment was faster in cone cells than rod cells as evident by 12-fold higher isomerhydrlase activity (RPE65 activity) in rod enriched chicken RPE compared to cone dominant bovine RPE after normalization of protein based on RPE65 levels (2).
RPE65 encodes a 65 kDa protein in microsomes of the retinal epithelium. Several mutations in RPE65 has been noted and described in RPE65 in patients with autosomal recessive, severe, childhood-onset retinal dystrophy. The longest open reading frame for RPE gene (2.4kb) encode a 533 amino acid protein with predicted molecular weight of 61 kDa, the protein after post-translational modification migrates at 65 kDa on reducing SDS-PAGE system. RPE65 sequence is highly conserved among various mammalian species Sequence comparison of RPE with other species suggest. A 4 bp deletion in putative Exxon 5 of the canine RPE65 gene led to an autosomal recessive, early onset and progressive retinal dystrophy in inbred kinship of Swedish briard beagle dogs due to a frame shift mutation resulting in an early stop codon making it an attractive model for human disease. The soluble form of RPE65 binds vitamin A preparing it for conversion to all-trans-retinyl ester via LRAT activity. The membrane bound form binds to all-trans-retinyl-esters making them available for processing by all-cis-retinol a reaction catalyzed by LRAT. The protein also undergo lipid modification during post-translational modification.

General readings

Rolling F, Le Meur G, Stieger K, Smith AJ, Weber M, Deschamps JY, Nivard D, Mendes-Madeira A, Provost N, Pereon Y, Cherel Y, Ali RR, Hamel C, Moullier P, Rolling F.
Gene therapeutic prospects in early onset of severe retinal dystrophy: restoration of vision in RPE65 Briard dogs using an AAV serotype 4 vector that specifically targets the retinal pigmented epithelium.
Bull Mem Acad R Med Belg. 2006;161(10-12):497-508; discussion 508-9.
PMID: 17503728

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RPE65 antibody

  Goat Polyclonal Antibody against RPE65  
TA303335 (0.5µg/ml) staining of Rat Retina lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RPE65 antibody

  Mouse Monoclonal RPE65 Antibody (401.8B11.3D9)  
Immunohistochemistry: RPE65 Antibody (401.8B11.3D9) - Immunohistochemical staining of RPE65 in mouse retina tissue using anti-RPE65. Mouse IgG1 401.8B11.3D9 Purified Bov, Hu, Ms, Por C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPE65 antibody

RPE65 staining of mouse retina tissue. Mouse IgG1 401.8B11.3D9 Purified Bov, Hu, Ms, Por C, ICC/IF, IP, P, WB
0.1 ml / €380.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

RPE65 - human gene knockout kit via CRISPR

RPE65 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RPE65 - human gene knockout kit via CRISPR

RPE65 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RPE65 - human gene knockout kit via CRISPR

RPE65 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RPE65 - human gene knockout kit via CRISPR

RPE65 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rpe65 - mouse gene knockout kit via CRISPR

Rpe65 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rpe65 - mouse gene knockout kit via CRISPR

Rpe65 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rpe65 - mouse gene knockout kit via CRISPR

Rpe65 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rpe65 - mouse gene knockout kit via CRISPR

Rpe65 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RPE65

  Recombinant protein of human retinal pigment epithelium-specific protein 65kDa (RPE65)  
RPE65 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RPE65 (mGFP-tagged)-Human retinal pigment epithelium-specific protein 65kDa (RPE65), 200 uL, >10^7 TU/mL

Lenti ORF particles, RPE65 (mGFP-tagged)-Human retinal pigment epithelium-specific protein 65kDa (RPE65), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, RPE65 (Myc-DDK-tagged)-Human retinal pigment epithelium-specific protein 65kDa (RPE65), 200 uL, >10^7 TU/mL

Lenti ORF particles, RPE65 (Myc-DDK-tagged)-Human retinal pigment epithelium-specific protein 65kDa (RPE65), 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Rpe65 (GFP-tagged ORF) - Rat retinal pigment epithelium 65 (Rpe65), 200 uL, >10^7 TU/mL

Lenti ORF particles, Rpe65 (GFP-tagged ORF) - Rat retinal pigment epithelium 65 (Rpe65), 200 uL, >10^7 TU/mL  
200 µl / €1,160.00
  OriGene Technologies, Inc.

Lenti ORF particles, Rpe65 (Myc-DDK-tagged ORF) - Rat retinal pigment epithelium 65 (Rpe65), 200 uL, >10^7 TU/mL

Lenti ORF particles, Rpe65 (Myc-DDK-tagged ORF) - Rat retinal pigment epithelium 65 (Rpe65), 200 uL, >10^7 TU/mL  
200 µl / €1,041.00
  OriGene Technologies, Inc.

16 Item(s)

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