RPL4 / RPL1 antibody
Principal name
RPL4 / RPL1 antibody
Alternative names for RPL4 / RPL1 antibody
60S ribosomal protein L4, 60S ribosomal protein L1
Gene ID
6124 (RPL4)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of RPL4 / RPL1 antibody
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Immunofluorescence of monoclonal antibody to RPL4 on HeLa cell. [antibody concentration 10 ug/ml]
RPL4 monoclonal antibody (M01), clone 4A3. Western Blot analysis of RPL4 expression in PC-12 ( Cat # L012V1 ).
RPL4 monoclonal antibody (M01), clone 4A3 Western Blot analysis of RPL4 expression in K-562 ( Cat # L009V1 ).
RPL4 monoclonal antibody (M01), clone 4A3. Western Blot analysis of RPL4 expression in Raw 264.7 ( Cat # L024V1 ).
Detection limit for recombinant GST tagged RPL4 is approximately 0.3ng/ml as a capture antibody.
Immunohistochemical of paraffin-embedded human lymphoma using 11302-1-AP(RPL4 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using RPL4 antibody 11302-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Rat brain tissue were subjected to SDS PAGE followed by western blot with 11302-1-AP( RPL4 Antibody) at dilution of 1:1000
K-562 cells were subjected to SDS PAGE followed by western blot with 11302-1-AP(RPL4 antibody) at dilution of 1:300
Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11302-1-AP( RPL4 Antibody) at dilution of 1:1000
Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11302-1-AP(RPL4 antibody) at dilution of 1:300
HeLa cells were subjected to SDS PAGE followed by western blot with 11302-1-AP(RPL4 antibody) at dilution of 1:300
K-562 cells were subjected to SDS PAGE followed by western blot with 11302-1-AP(RPL4 antibody) at dilution of 1:800
Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11302-1-AP( RPL4 Antibody) at dilution of 1:1000
U-937 cells were subjected to SDS PAGE followed by western blot with 11302-1-AP(RPL4 antibody) at dilution of 1:300
A375 cells were subjected to SDS PAGE followed by western blot with 11302-1-AP(RPL4 antibody) at dilution of 1:300
IP Result of anti-RPL4 (IP:11302-1-AP, 3ug; Detection:11302-1-AP 1:800) with K-562 cells lysate 6000ug.
Western Blot: RPL4 Lysate [NBL1-15539] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for RPL4
RPL4 MaxPab rabbit polyclonal antibody. Western Blot analysis of RPL4 expression in human colon.
Immunohistochemistry-Paraffin: RPL4 Antibody [NBP1-81329] - Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine pancreas.
Immunofluorescence: RPL4 Antibody [NBP1-81329] Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, nucleoli & cytoplasm.
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RPL4(GTX112184) antibody at 1:500 dilution.
RPL4 antibody detects RPL4 protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. RPL4 antibody (GTX112184) dilution: 1:500.
RPL4 antibody detects RPL4 protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: RPL4 protein stained by RPL4 antibody (GTX112184) diluted at 1:500.Blue: Hoechst 33342 staining.
Sample (30 ug of whole cell lysate) A: Raji B: K562 C: NCI-H929 10% SDS PAGE GTX112184 diluted at 1:1000
RPL4 antibody detects RPL4 protein at cytosol on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. RPL4 antibody (GTX112184) dilution: 1:500.
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence of monoclonal antibody to RPL4 on HeLa cell (antibody concentration 10 ug/ml).
RPL4 monoclonal antibody (M01), clone 4A3. Western Blot analysis of RPL4 expression in PC-12.
RPL4 monoclonal antibody (M01), clone 4A3 Western Blot analysis of RPL4 expression in K-562.
RPL4 monoclonal antibody (M01), clone 4A3. Western Blot analysis of RPL4 expression in Raw 264.7.
Detection limit for recombinant GST tagged RPL4 is approximately 0.3 ng/ml as a capture antibody.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM33414PU-N | RPL4 / RPL1 (251-350) antibody |
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Mouse | IgG2a | 4A3 | Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
50 µg /
€530.00
|
| Acris Antibodies GmbH | |||||||
 +5 additional images |
Highly validated antibody! |
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| H00006124-M01 | RPL4 / RPL1 antibody |
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![Immunofluorescence of monoclonal antibody to RPL4 on HeLa cell. [antibody concentration 10 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00006124-M01-4-1-1-L.jpg) |
Mouse | IgG2a | 4A3 | Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +4 additional images |
Highly validated antibody! |
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| H00006124-A01 | RPL4 / RPL1 antibody |
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 |
Mouse | Serum | Hu | E, WB |
50 µl /
€290.00
|
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| Abnova Taiwan Corp. | |||||||
| H00006124-D01P | RPL4 / RPL1 antibody |
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 |
Rabbit | Purified | Hu | E(detection), WB |
0.1 mg /
€380.00
|
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| Abnova Taiwan Corp. | |||||||
| NBP1-81329 | RPL4 / RPL1 antibody |
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![Immunohistochemistry-Paraffin: RPL4 Antibody [NBP1-81329] - Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine pancreas.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/RPL4 Antibody-Immunohistochemistry-Paraffin-NBP1-81329-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| H00006124-Q01 | RPL4 / RPL1 |
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Human |
2 µg /
€300.00
|
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| Abnova Taiwan Corp. | |||||
| H00006124-Q01-10 | RPL4 / RPL1 |
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|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00006124-Q01-25 | RPL4 / RPL1 |
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|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
