RPLP0 antibody

Principal name

RPLP0 antibody

Alternative names for RPLP0 antibody

60S acidic ribosomal protein P0, 60S ribosomal protein L10E

SwissProt ID

O74864 (Schpo), P05317 (Yeast), P05388 (Human), P14869 (Mouse), P19889 (Drome), P19945 (Rat), P47826 (Chick), Q29214 (Pig), Q95140 (Bovin), Q9PV90 (Danre)

Gene ID

6175 (RPLP0)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of RPLP0 antibody

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. The ribosomal protein is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

General readings

Rinne,T., (2008) Hum. Mol. Genet. 17 (13), 1968-1977

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RPLP0 antibody

Predicted band size: 34 kDa . Positive control: NIH/3T3, A431 and Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: NIH/3T3 cell lysate Lane2: A431 cell lysate Lane3: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPLP0 antibody

Predicted band size: 34 kDa . Positive control: Hela, 293T, A431 and Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: 293T cell lysate Lane3: A431 cell lysate Lane4: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPLP0 antibody

Western blot analysis of extracts of various cell lines, using RPLP0 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RPLP0 antibody

WB Suggested Anti-RPLP0 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate.RPLP0 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RPLP0 antibody

WB Suggested Anti-RPLP0 Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat cell lysate.RPLP0 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RPLP0 (N-term) antibody

Western blot analysis of RPLP0 Antibody (N-term) Cat.-No AP53726PU-N in A375 cell line lysates (35ug/lane).
This demonstrates the RPLP0 antibody detected the RPLP0 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RPLP0 antibody

Immunofluorescence of purified MaxPab antibody to RPLP0 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RPLP0 antibody

RPLP0 Mouse IgG2a 1B4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RPLP0 antibody

Human Bronchial Epithelial Tissue; Rabbit Anti-RPLP0 Antibody . Catalog Number: ARP40221_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue . Observed Staining: Cytoplasmic . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-RPLP0 Antibody (ARP40221_P050) ) in Human Bronchial Epithelial Tissue using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

RPLP0 antibody

Human Jurkat; WB Suggested Anti-RPLP0 Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate. RPLP0 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; RPLP0 antibody - middle region (ARP40222_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

RPLP0 antibody

Western Blot: RPLP0 Antibody [NBP1-49979] - Detection of Human and Mouse RPLP0 by Western Blot (h&m) and Immunoprecipitation (h).  Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-RPLP0 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  RPLP0 was also immunoprecipitated by rabbit anti-RPLP0 antibody which recognizes an upstream epitope.  For blotting immunoprecipitated RPLP0 was used.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). Rabbit Aff - Purified Hu, Ms IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

RPLP0 antibody

Western Blot: RPLP0 Antibody [NBP1-57528] - This Anti-RPLP0 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 2.5ug/ml. Rabbit Purified Can, Hu WB
0.1 mg / €360.00
  Novus Biologicals Inc.

RPLP0 antibody

Immunohistochemistry-Paraffin: RPLP0 Antibody [NBP1-82859]  - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RPLP0 (transcript variant 2)

RPLP0 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

RPLP0

RPLP0 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

RPLP0

RPLP0 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

RPLP0

RPLP0 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RPLP0

RPLP0 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

RPLP0

RPLP0 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

RPLP0

RPLP0 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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