RPS27 antibody

Principal name

RPS27 antibody

Alternative names for RPS27 antibody

MPS1, Metallopan-stimulin 1, MPS-1, 40S ribosomal protein S27

SwissProt ID

P42677 (Human), Q2KHT7 (Bovin), Q6ZWU9 (Mouse), Q71TY3 (Rat)

Gene ID

6232 (RPS27), 57294 (Rps27), 100043813 (Gm9846), 94266

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Mky (Monkey), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of RPS27 antibody

MPS1, also known as RPS27. It is a ribosomal protein. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. MPS1 is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

General readings

Fernandez-Pol JA, Klos DJ, Hamilton PD.
A growth factor-inducible gene encodes a novel nuclear protein with zinc finger structure.
J Biol Chem. 1993 Oct 5;268(28):21198-204.
PMID: 8407955

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RPS27 (N-term) antibody

  Goat Polyclonal Antibody against RPS27  
TA302828 (0.05µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RPS27 antibody

  Rabbit Polyclonal Anti-RPS27 Antibody  
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Jurkat cells, K562 cells, Raji cells, Primary antibody: (RPS27 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPS27 antibody

Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and human brain (right) tissues using MPS1 antibody Cat.-No AM06342SU-N with DAB staining. Mouse IgG1 4A12 Ascites Hu E, P
0.1 ml / €400.00
  Acris Antibodies GmbH

RPS27 antibody

Confocal immunofluorescence analysis of Hela cells (A), BCBL-1 cells (B) and L1210 cells (C) using MPS1 antibody Cat.-No AM06343SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 7E3 Ascites Hu E, ICC/IF
0.1 ml / €400.00
  Acris Antibodies GmbH

RPS27 antibody

RPS27 polyclonal antibody ( Cat # PAB6240 ) staining ( 0.05 µg/ml ) of Jurkat lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

RPS27 antibody

Western blot analysis of RPS27 expression in HepG2 (A); NIH3T3 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

RPS27 antibody

Western blot analysis of RPS27 expression in HepG2 (A); NIH3T3 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

RPS27 antibody

Western blot analysis of RPS27 expression in HepG2 (A); NIH3T3 (B); H9C2 (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

Rps27 - mouse gene knockout kit via CRISPR

Rps27 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rps27 - mouse gene knockout kit via CRISPR

Rps27 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rps27 - mouse gene knockout kit via CRISPR

Rps27 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rps27 - mouse gene knockout kit via CRISPR

Rps27 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rps27rt - mouse gene knockout kit via CRISPR

Rps27rt - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rps27rt - mouse gene knockout kit via CRISPR

Rps27rt - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rps27rt - mouse gene knockout kit via CRISPR

Rps27rt - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rps27rt - mouse gene knockout kit via CRISPR

Rps27rt - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RPS27

RPS27 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

RPS27 overexpression lysate

RPS27 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RPS27 (mGFP-tagged)-Human ribosomal protein S27 (RPS27), 200 uL, >10^7 TU/mL

Lenti ORF particles, RPS27 (mGFP-tagged)-Human ribosomal protein S27 (RPS27), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, RPS27 (Myc-DDK-tagged)-Human ribosomal protein S27 (RPS27), 200 uL, >10^7 TU/mL

Lenti ORF particles, RPS27 (Myc-DDK-tagged)-Human ribosomal protein S27 (RPS27), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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