RPS27A antibody

Principal name

RPS27A antibody

Alternative names for RPS27A antibody

UBA80, UBCEP1, Ubiquitin-40S ribosomal protein S27a, Ubiquitin carboxyl extension protein 80

SwissProt ID

P0C016 (Schpo), P62979 (Human), P62982 (Rat), P62983 (Mouse), P62992 (Bovin), P79781 (Chick)

Gene ID

6233 (RPS27A), 78294 (Rps27a), 81777

Available reactivities

Hu (Human), Bov (Bovine), Ce (C. elegans), Chk (Chicken), Dros (Drosophila), Ms (Mouse), Pl (Plant), Rt (Rat), Fe (Feline), Prim (Primate), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of RPS27A antibody

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translatiolly processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple altertively spliced transcript variants that encode the same proteins have been identified.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RPS27A antibody

  Mouse Monoclonal Ubiquitin Antibody (Ubi-1)  
Western Blot: Ubiquitin Antibody (Ubi-1) - Staining of ubiquitin using anti-Ubiquitin.  Lane 1 is mono-ubiquitin and polyubiquitin chains. Lane 2 is monoubiquitin only (overloaded). Lanes 3,4 and 5 are 0.1 mg rat cerebellum, cortex, and brain stem respectively.  20% gels with PVDF blots (Nitrocellulose works but not as well). Mouse IgG1 Ubi-1 Purified Bov, Ce, Chk, Dros, Hu, Ms, Pl C, E, ICC/IF, WB
50 µl / €325.00
  OriGene Technologies, Inc.

RPS27A antibody

  Rabbit Polyclonal Ubiquitin Antibody  
Immunofluorescent: Ubiquitin Antibody - Immunofluorescent staining of aggresomes of mouse L-cells treated with lactacysteine, using anti-Ubiquitin. Rabbit Purified Bov, Ce, Chk, Dros, Hu, Ms C, E, ICC/IF, WB
50 µl / €325.00
  OriGene Technologies, Inc.

RPS27A antibody

  Anti-RPS27A Rabbit Polyclonal Antibody  
Predicted band size: 9 kDa . Positive control: HL-60 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPS27A antibody

  Rabbit Polyclonal Ubiquitin/Ubiquitin 1 Antibody  
Western Blot: Ubiquitin Antibody [TA337188] - (1:1000) Analysis in various human cell lines: HeLa, Jurkat, Daudi, 293, Rh30, A375, T98G, HCT-116, and Hep-3. Ubiquitin migrates at ~8-8.5 kDa.  Higher bands observed in HeLa, 293, T98G and HCT-116 correspond Rabbit Purified Fe, Hu, Ms, Prim, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPS27A antibody

  Rabbit Polyclonal Anti-RPS27A Antibody  
RPS27A Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

RPS27A - human gene knockout kit via CRISPR

RPS27A - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

RPS27A - human gene knockout kit via CRISPR

RPS27A - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

RPS27A - human gene knockout kit via CRISPR

RPS27A - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

RPS27A - human gene knockout kit via CRISPR

RPS27A - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rps27a - mouse gene knockout kit via CRISPR

Rps27a - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rps27a - mouse gene knockout kit via CRISPR

Rps27a - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rps27a - mouse gene knockout kit via CRISPR

Rps27a - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rps27a - mouse gene knockout kit via CRISPR

Rps27a - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RPS27A (full length, N-term HIS tag)

  Recombinant protein of human ribosomal protein S27a (RPS27A), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
RPS27A Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, RPS27A (mGFP-tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, RPS27A (mGFP-tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, RPS27A (mGFP-tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, RPS27A (mGFP-tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, RPS27A (mGFP-tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, RPS27A (mGFP-tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, RPS27A (Myc-DDK tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, RPS27A (Myc-DDK tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Lenti ORF particles, RPS27A (Myc-DDK tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, RPS27A (Myc-DDK tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Lenti ORF particles, RPS27A (Myc-DDK tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 3 , 200 uL, >10^7 TU/mL

Lenti ORF particles, RPS27A (Myc-DDK tagged) - Human ribosomal protein S27a (RPS27A), transcript variant 3 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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