RPS27A antibody

Principal name

RPS27A antibody

Alternative names for RPS27A antibody

UBA80, UBCEP1, Ubiquitin-40S ribosomal protein S27a, Ubiquitin carboxyl extension protein 80

SwissProt ID

P0C016 (Schpo), P62979 (Human), P62982 (Rat), P62983 (Mouse), P62992 (Bovin), P79781 (Chick)

Gene ID

6233 (RPS27A)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Ce (C. elegans), Chk (Chicken), Dros (Drosophila), Pl (Plant), Fe (Feline), Prim (Primate), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)

Background of RPS27A antibody

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq]

18 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RPS27A antibody

Western Blot: Ubiquitin Antibody (Ubi-1) - Staining of ubiquitin using anti-Ubiquitin.  Lane 1 is mono-ubiquitin and polyubiquitin chains. Lane 2 is monoubiquitin only (overloaded). Lanes 3,4 and 5 are 0.1 mg rat cerebellum, cortex, and brain stem respectively.  20% gels with PVDF blots (Nitrocellulose works but not as well). Mouse IgG1 Ubi-1 Purified Bov, Ce, Chk, Dros, Hu, Ms, Pl C, E, ICC/IF, WB
50 µl / €325.00
  OriGene Technologies, Inc.

RPS27A antibody

Immunofluorescent: Ubiquitin Antibody - Immunofluorescent staining of aggresomes of mouse L-cells treated with lactacysteine, using anti-Ubiquitin. Rabbit Purified Bov, Ce, Chk, Dros, Hu, Ms C, E, ICC/IF, WB
50 µl / €325.00
  OriGene Technologies, Inc.

RPS27A antibody

Predicted band size: 9 kDa . Positive control: HL-60 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPS27A antibody

Western Blot: Ubiquitin Antibody [TA337188] - (1:1000) Analysis in various human cell lines: HeLa, Jurkat, Daudi, 293, Rh30, A375, T98G, HCT-116, and Hep-3. Ubiquitin migrates at ~8-8.5 kDa.  Higher bands observed in HeLa, 293, T98G and HCT-116 correspond Rabbit Purified Fe, Hu, Ms, Prim, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPS27A antibody

RPS27A Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RPS27A antibody

RPS27A Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

RPS27A antibody

Western Blot analysis of RPS27A expression in transfected 293T cell line (H00006233-T02) by RPS27A MaxPab polyclonal antibody.

Lane 1: RPS27A transfected lysate(18.00 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RPS27A antibody

RPS27A monoclonal antibody (M01), clone 3E2-E6 Western Blot analysis of RPS27A expression in HL-60 ( Cat # L014V1 ). Mouse IgG1 3E2-E6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RPS27A antibody

Detection limit for recombinant GST tagged RPS27A is approximately 3ng/ml as a capture antibody. Mouse IgG2a 2G10 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

RPS27A antibody

RPS27A Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RPS27A (full length, N-term HIS tag)

RPS27A Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

RPS27A

RPS27A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
10 µg / €380.00
  Abnova Taiwan Corp.

RPS27A

RPS27A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
25 µg / €510.00
  Abnova Taiwan Corp.

RPS27A

RPS27A Human 12.5% SDS-PAGE Stained with Coomassie Blue
10 µg / €380.00
  Abnova Taiwan Corp.

RPS27A

RPS27A Human 12.5% SDS-PAGE Stained with Coomassie Blue
25 µg / €510.00
  Abnova Taiwan Corp.

RPS27A

RPS27A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RPS27A

RPS27A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

RPS27A

Western Blot: Ubiquitin Protein [NBP1-42465] - The anti-Ubiquitin antibody NB300-129 was used to detect this protein on a western blot.  500ng of protein were loaded on the gel and then detected with NB300-129. Human Purified
10 µg / €420.00
  Novus Biologicals Inc.

18 Item(s)

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