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RPS3A antibody

Principal name

RPS3A antibody

Alternative names for RPS3A antibody

40S ribosomal protein S3a, FTE1, Fte-1, MFTL, v-fos transformation effector protein

SwissProt ID

P33442 (Yeast), P48154 (Caeel), P49242 (Rat), P55830 (Drome), P61246 (Felca), P61247 (Human), P97351 (Mouse), Q09781 (Schpo), Q56JV9 (Bovin), Q801S3 (Xenla)

Gene ID

6189 (RPS3A), 8618983 (ap2a1-1)

Ncbi ID

9606, NP_000997, NP_000997.1, NM_001006

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Ze (Zebrafish), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP)

Background of RPS3A antibody

RPS3A may play a role during erythropoiesis through regulation of transcription factor DDIT3.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 R species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also med v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing altertive transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar R genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

General readings

Wool IG., et al. (1995) Biochem Cell Biol. 73(11):933-47.Nolte D., et al. (1996) Gene. 169(2): 179-85.

RPS3A polyclonal antibody (A01), Lot # ONW0060316QCS1. Western Blot analysis of RPS3A expression in NIH/3T3.

H00006189-A01

RPS3A polyclonal antibody (A01), Lot # ONW0060316QCS1. Western Blot analysis of RPS3A expression in Raw 264.7.

H00006189-A01

RPS3A polyclonal antibody (A01), Lot # ONW0060316QCS1. Western Blot analysis of RPS3A expression in PC-12.

H00006189-A01

RPS3A polyclonal antibody (A01), Lot # ONW0060316QCS1 Western Blot analysis of RPS3A expression in HeLa ( Cat # L013V1 ).

H00006189-A01

RPS3A antibody detects RPS3A protein at cytosol on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. RPS3A antibody (GTX105020) dilution: 1:200.

GTX105020

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ribosomal protein S3a(GTX105020) antibody at 1:200 dilution.

GTX105020

RPS3A antibody detects RPS3A protein by Western blot analysis.A. 30 µg PC-12 whole cell lysate/extractB. 30 µg Rat2 whole cell lysate/extract12 % SDS-PAGERPS3A antibody (GTX105020) dilution: 1:1000

GTX105020

RPS3A antibody detects RPS3A protein by Western blot analysis.A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract12 % SDS-PAGERPS3A antibody (GTX105020) dilution: 1:1000

GTX105020

Sample(30 ug whole cell lysate)A: 293TB: A431 (GTX27909)C: H1299D: HeLa S3 (GTX14654) E: Hep G2 (GTX27900) F: MOLT4 (GTX27912)G: Raji (GTX27908)12% SDS PAGEGTX105020 diluted at 1:1000

GTX105020

Immunohistochemical of paraffin-embedded human pancreas cancer using 14123-1-AP(RPS3A antibody) at dilution of 1:100 (under 10x lens)

14123-1-AP

Immunohistochemical of paraffin-embedded human pancreas cancer using 14123-1-AP(RPS3A antibody) at dilution of 1:100 (under 40x lens)

14123-1-AP

Immunofluorescent analysis of HepG2 cells, using RPS3A antibody 14123-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

14123-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 14123-1-AP(RPS3A antibody) at dilution of 1:800

14123-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 14123-1-AP(RPS3A antibody) at dilution of 1:800

14123-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 14123-1-AP(RPS3A antibody) at dilution of 1:800

14123-1-AP

A549 cells were subjected to SDS PAGE followed by western blot with 14123-1-AP(RPS3A antibody) at dilution of 1:800

14123-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 14123-1-AP(RPS3A antibody) at dilution of 1:800

14123-1-AP

Raji cells were subjected to SDS PAGE followed by western blot with 14123-1-AP(RPS3A antibody) at dilution of 1:800

14123-1-AP

Raji cells were subjected to SDS PAGE followed by western blot with 14123-1-AP(RPS3A antibody) at dilution of 1:800

14123-1-AP

IP Result of anti-RPS3A (IP:14123-1-AP, 4ug; Detection:14123-1-AP 1:500) with HepG2 cells lysate 3600ug.

14123-1-AP

1X10^6 HepG2 cells were stained with 0.2ug RPS3A antibody (14123-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

14123-1-AP

Western Blot: RPS3A Antibody [NBP1-31598] - Western Blot analysis of NBP1-31598 on 293T (Lane A), A431 (Lane B), H1299 (Lane C), HeLa S3 (Lane D), Hep G2 (Lane E), MOLT4 (Lane F), and Raji (Lane G), with 12% SDS PAGE, diluted at 1:1000.

NBP1-31598

Arabidopsis thaliana; . Sample Type: Arabidopsis thaliana extract (30ug). Primary Diltution: 1:1000. Secondary Antibody: anti-rabbit HRP. Secondary Dilution: 1:15,000. Exposure Time: 1 minute. Image Submitted By: Anonymous .; RPS3A antibody - N-terminal region (ARP56138_P050) in Arabidopsis thaliana cells using Western Blot

ARP56138_P050

Human Liver; WB Suggested Anti-RPS3A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Liver; RPS3A antibody - N-terminal region (ARP56138_P050) in Human Liver cells using Western Blot

ARP56138_P050

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using RPS3A antibody(GTX111935) antibody at 1:500 dilution.

GTX111935

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using RPS3A antibody(GTX111935) antibody at 1:500 dilution.

GTX111935

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPS3A(GTX111935) antibody at 1:500 dilution.

GTX111935

Sample (30 ug of whole cell lysate) A: A431 B: Raji 12% SDS PAGE GTX111935 diluted at 1:1000

GTX111935

10ug of GTX111935-pro RPS3A recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

GTX111935-pro

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA308427	RPS3A antibody
	Rabbit	IgG	Aff - Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 ml / €325.00
						OriGene Technologies, Inc.
+4 additional images	Highly validated antibody!
H00006189-A01	RPS3A antibody
	Mouse		Serum	Hu, Ms, Rt	E, WB	50 µl / €290.00
						Abnova Taiwan Corp.
+3 additional images	Highly validated antibody!
TA336162	RPS3A antibody
	Rabbit	IgG	Purified	Bov, Can, Hu, Ms, Por, Ze	WB	50 µg / €325.00
						OriGene Technologies, Inc.
+1 additional image
ARP56138_P050	RPS3A antibody
	Rabbit		Aff - Purified	Can, Hu, Ms, Por, Rt, Ze	WB	50 µg / €350.00
						AVIVA Systems Biology
+1 additional image
NBP1-31598	RPS3A antibody
	Rabbit	IgG		Hu	ICC/IF, P, WB	0.1 mg / €420.00
						Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
AR50402PU-N	RPS3A (1-264, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.5 mg / €730.00
					Acris Antibodies GmbH
AR50402PU-S	RPS3A (1-264, His-tag)
	Human	Purified	> 90 % by SDS - PAGE	E. coli	0.1 mg / €300.00
					Acris Antibodies GmbH
H00006189-P01-10	RPS3A
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.		10 µg / €380.00
					Abnova Taiwan Corp.
H00006189-P01-25	RPS3A
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.		25 µg / €510.00
					Abnova Taiwan Corp.
H00006189-Q01-10	RPS3A
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.	10 µg / €380.00
					Abnova Taiwan Corp.
H00006189-Q01-25	RPS3A
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.	25 µg / €510.00
					Abnova Taiwan Corp.

Lysates (Overexpressed)

Catalog No.
LY423675	RPS3A overexpression lysate
		0.1 mg / €280.00
		OriGene Technologies, Inc.

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