RPS6KA1 antibody

Principal name

RPS6KA1 antibody

Alternative names for RPS6KA1 antibody

Ribosomal S6 kinase 1, 90 kDa ribosomal protein S6 kinase 1, MAPKAPK1A, RSK1, RSK-1, p90RSK1, Ribosomal protein S6 kinase alpha-1

SwissProt ID

P18653 (Mouse), Q15418 (Human), Q505N6 (Mouse), Q63531 (Rat)

Gene ID

6195 (RPS6KA1), 20111

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Chk (Chicken), Eq (Equine), Op (Opossum), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Dot blot (Dot), Frozen Sections (C)

Background of RPS6KA1 antibody

RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2 (MAPKAP-K1b), RSK3 (MAPKAP-K1c), and RSK4. RSK proteins are substrates for and downstream transducers of MAPK signaling proteins, primarily ERK1&2. RSK proteins possess two separate kinase domains, the C-terminal kinase domain and the N-terminal kinase domain, separated by a linker region containing a hydrophobic motif. Activation of RSK1 is a multi-step process involving phosphorylation of multiple residues within the three domains, changes in RSK protein localization, and modulation of RSK complex formation with ERK1&2 and/or 14-3-3 beta proteins. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation. ERK 1&2 phosphorylates threonine 573 of the C-terminal kinase domain, thereby stimulating this kinase activity and leading to phosphorylation of serine 380 within the linker region. Phosphoserine 380 is part of a docking site for PDK1, a constitutively active cytoplasmic kinase that phosphorylates serine 221 and thereby activates the N-terminal kinase domain to phosphorylate various substrates that include transporters, transcription factors, and transcription co-activators.

General readings

General / background references:Roux PP et al. Phosphorylation of p90 ribosomal S6 kinase (RSK) regulates extracellular signal-regulated kinase docking and RSK activity. Mol Cell Biol 23:4796-804 (2003). PubMed PMID: 12832467
Kim KW et al. Extracellular signal-regulated kinase/90-KDA ribosomal S6 kinase/nuclear factor-kappa B pathway mediates phorbol 12-myristate 13-acetate-induced megakaryocytic differentiation of K562 cells. J Biol Chem 276:13186-91 (2001). PubMed PMID: 11278385
Richards SA et al. Characterization of regulatory events associated with membrane targeting of p90 ribosomal S6 kinase 1. Mol Cell Biol 21:7470-80 (2001). PubMed PMID: 11585927
Zhang Y et al. UVA induces Ser381 phosphorylation of p90RSK/MAPKAP-K1 via ERK and JNK pathways. J Biol Chem 276:14572-80 (2001). PubMed PMID: 11278279

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RPS6KA1 antibody

Immunohistochemistry: Rsk1 Antibody [NB110-57496] - IHC-P Colon tissue section stained for RSK-1 subcellular location using anti-RSK-1. Rabbit IgG E4 Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

RPS6KA1 antibody

Western blot analysis of RPS6KA1 (arrow) using rabbit polyclonal RPS6KA1 Antibody (S732). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6KA1 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RPS6KA1 antibody

Western blot analysis of RPS6K1 in Jurkat cell lysate with RPS6K1 antibody at (A) 2.5, (B) 5 and (C) 10 ug/mL. Rabbit IgG Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RPS6KA1 antibody

p90 RSK1 antibody detects p90 RSK1 protein by Western blot analysis. A. 30 ug HepG2 whole cell lysate/extract. 7.5 % SDS-PAGE. p90 RSK1 antibody (TA308953) dilution: 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPS6KA1 antibody

Predicted band size: 83 kDa . Positive control: Hela and NIH/3T3 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: Hela and NIH/3T3 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPS6KA1 antibody

Western blot analysis of extracts from 3T3 cells, treated with PMA (125ng/ml, 30mins), using p90 RSK (Ab-573) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPS6KA1 antibody

Western blot analysis of P90 RSK1 immuno-precipitated from the mouse brain extract (right) and using RSK1-HRP as a probe. Immuno-precipitated negative control (left). Rabbit IgG Aff - Purified Bov, Hu, Ms, Rt E, IP, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

RPS6KA1 antibody

HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-p90 Rsk antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. In addition to p90 Rsk, this antibody recognizes a non-specific protein with a molecular weight of approximately 66 kD. Rabbit IgG Poly6048 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

RPS6KA1 antibody

Immunohistochemical analysis of paraffin-embedded human small intestine tissues uing RSK1  antibody Cat.-No AM06174SU-N Mouse IgG2a 10B1D7A9 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

RPS6KA1 pThr348 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p90RSK (phospho-Thr348) antibody (AP02397PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

RPS6KA1 pThr348 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p90RSK (phospho-Thr348) antibody (AP02397PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

RPS6KA1 antibody

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using p90RSK antibody AP02663PU (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

RPS6KA1 antibody

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using p90RSK antibody AP02663PU (Red). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

RPS6KA1 antibody

Figure 1. Western blot analysis of extract from HeLa cells, untreated or treated with PMA (200 nM, 30 min), using p90RSK antibody AP02664PU. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €270.00
  Acris Antibodies GmbH

RPS6KA1 antibody

Figure 1. Western blot analysis of extract from HeLa cells, untreated or treated with PMA (200 nM, 30 min), using p90RSK antibody AP02664PU. Rabbit Aff - Purified Hu, Ms WB
50 µl / €230.00
  Acris Antibodies GmbH

RPS6KA1 antibody

RPS6KA1 Rabbit IgG Purified Bov, Can, Chk, Hu, Ms, Rt WB
0.1 ml / €510.00
  Acris Antibodies GmbH

RPS6KA1 antibody

Immunohistochemistry (IHC) analyzes of p90 RSK antibody (Cat.-No.: AP06435PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

RPS6KA1 antibody

Immunohistochemistry (IHC) analyzes of p90 RSK antibody (Cat.-No.: AP06439PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

RPS6KA1 antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RPS6KA1 (N-term) pThr368/pSer372 antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu ICC/IF, P
50 µl / €470.00
  Acris Antibodies GmbH

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