RPS6KA3 / RSK2 antibody

Principal name

RPS6KA3 / RSK2 antibody

Alternative names for RPS6KA3 / RSK2 antibody

RSK-2, ISPK1, ISPK-1, MAPKAPK1, MAPKAPK-1b, S6K-alpha-3, p90RSK3, Ribosomal S6 kinase 2, Ribosomal protein S6 kinase alpha-3

SwissProt ID

P18654 (Mouse), P51812 (Human)

Gene ID

6197 (RPS6KA3), 5768634 (IPR010451)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)

Background of RPS6KA3 / RSK2 antibody

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RPS6KA3 / RSK2 antibody

Immunofluorescence analysis of HepG2 cells using RSK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 4E10 Ascites Hu E, ICC/IF, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

RPS6KA3 / RSK2 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between RPS6KA3 and MAPK1 HeLa cells were stained with anti-RPS6KA3 rabbit purified polyclonal 1:1200 and anti-MAPK1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RPS6KA3 / RSK2 (C-term) antibody

Western blot of HeLa lysate showing specific immunolabeling of the ~90k RSK2 protein. Rabbit IgG Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RPS6KA3 / RSK2 (N-term) antibody

RSK3 Antibody (N-term) (Cat. #TA325008) western blot analysis in Ramos cell line and mouse thymus tissue lysates (35ug/lane).This demonstrates the RSK3 antibody detected the RSK3 protein (arrow). Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

RPS6KA3 / RSK2 antibody

RPS6KA3 / RSK2 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

RPS6KA3 / RSK2 pSer369 antibody

Dot blot analysis of anti-RPS6KA3-pSer369 Phospho-specific Pab (Cat#AP12878PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

RPS6KA3 / RSK2 (604-617) antibody

Human Tonsil (formalin-fixed, paraffin-embedded) stained with RPS6KA3 antibody AP22881PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt, Tr IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RPS6KA3 / RSK2 antibody

RPS6KA3 polyclonal antibody (A01), Lot # 060707JCS1 Western Blot analysis of RPS6KA3 expression in SJCRH30 ( Cat # L027V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RPS6KA3 / RSK2 antibody

Immunofluorescence of purified MaxPab antibody to RPS6KA3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

RPS6KA3 / RSK2 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and RPS6KA3 HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2b 2G10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RPS6KA3 / RSK2 pSer369 antibody

Dot blot analysis of RPS6KA3 (phospho S369) polyclonal antibody ( Cat # PAB8135 ) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml. Rabbit Hu Dot, E
0.4 ml / €400.00
  Abnova Taiwan Corp.

RPS6KA3 / RSK2 antibody

Western Blot:   Samples: RSK2 Antibody [NBP1-30107] - WB analysis of whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells using NBP1-30107 used for WB at 0.4 mcg/ml. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €440.00
  Novus Biologicals Inc.

RPS6KA3 / RSK2 antibody

Immunohistochemistry-Paraffin: RSK2 Antibody [NBP1-85552] - Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RPS6KA3 / RSK2

RPS6KA3 / RSK2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RPS6KA3 / RSK2

RPS6KA3 / RSK2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

RPS6KA3 / RSK2

RPS6KA3 / RSK2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

RPS6KA3 / RSK2

RPS6KA3 / RSK2 Human
2 µg / €300.00
  Abnova Taiwan Corp.

RPS6KA3 / RSK2

RPS6KA3 / RSK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RPS6KA3 / RSK2

RPS6KA3 / RSK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

RPS6KA3 / RSK2

RPS6KA3 / RSK2 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

Items 1 to 20 of 24 total

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