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RPS6KA5 / MSK1 antibody

Principal name

RPS6KA5 / MSK1 antibody

Alternative names for RPS6KA5 / MSK1 antibody

Ribosomal protein S6 kinase alpha-5, RSKL, Nuclear mitogen- and stress-activated protein kinase 1, 90 kDa ribosomal protein S6 kinase 5, RSK-like protein kinase

SwissProt ID

O75582 (Human), Q5F3L1 (Chick), Q8C050 (Mouse)

Gene ID

9252 (RPS6KA5)

Ncbi ID

NP_004746.2, 9606, NP_004746, NM_182398, NP_872198

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Mky (Monkey), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of RPS6KA5 / MSK1 antibody

The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. A related S6 kinase, p70 S6 kinase, functions to phosphorylate the S6 protein on ribosomal 40S subunits. p70 S6 kinase beta shares high sequence homology with p70 S6 kinase, except in the carboxy terminus where it contains a proline-rich domain that may be involved in SH3 domain containing protein interactions. MSK1 (also designated RLPK) is related to Rsk and p70 S6 kinase family members and is thought to be structurally similar to Rsk family members, but it may be regulated by distinct mechanisms.

Figure 1. Western blot analysis of extract from HuVec cells treated or untreated with PMA (200 nM, 30 min), using MSK1 antibody AP02710PU.

Immunohistochemistry (IHC) analysis of MSK1 antibody (Cat.-No.: AP06539PU-N) in paraffin-embedded human breast carcinoma tissue.

AP06539PU-N

Western blot (WB) analysis of MSK1 antibody (Cat.-No.: AP06539PU-N) at 1/500 dilution
Lane1:Jurkat whole cell lysate
Lane2:Mouse liver tissue lysate
Lane3:Rat liver tissue lysate

AP06539PU-N

Proximity Ligation Analysis (PLA) of protein-protein interactions between NR4A1 and RPS6KA5 HeLa cells were stained with anti-NR4A1 rabbit purified polyclonal 1:1200 and anti-RPS6KA5 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00009252-M01

Western Blot analysis of RPS6KA5 expression in transfected 293T cell line by RPS6KA5 monoclonal antibody (M01), clone 2B11.Lane 1: RPS6KA5 transfected lysate(89.9 KDa).Lane 2: Non-transfected lysate.

H00009252-M01

RPS6KA5 monoclonal antibody (M01), clone 2B11. Western Blot analysis of RPS6KA5 expression in HeLa ( Cat # L013V1 ).

H00009252-M01

Western blot (WB) analysis of MSK1 antibody (Cat.-No.: AP01343PU-N) in extracts from HT-29 cells.

AP01343PU-N

Proximity Ligation Analysis (PLA) of protein-protein interactions between RPS6KA5 and EGF HeLa cells were stained with anti-RPS6KA5 rabbit purified polyclonal 1:1200 and anti-EGF mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00009252-D01P

RPS6KA5 MaxPab rabbit polyclonal antibody. Western Blot analysis of RPS6KA5 expression in rat brain.

H00009252-D01P

Western Blot analysis of RPS6KA5 expression in transfected 293T cell line (H00009252-T02) by RPS6KA5 MaxPab polyclonal antibody.Lane 1: RPS6KA5 transfected lysate(89.90 KDa).Lane 2: Non-transfected lysate.

H00009252-D01P

Immunohistochemistry analyzes of MSK1 antibody (Cat.-No.: AP20352PU-N) in paraffin-embedded human lymph node tissue.

AP20352PU-N

Western blot analysis of MSK1 antibody (Cat.-No.: AP20352PU-N) in extracts from 293 cells with treatment of Etoposide 25uM 60min.

AP20352PU-N

Immunohistochemistry (IHC) analyzes of MSK1 antibody (Cat.-No.: AP21118PU-N) in paraffin-embedded human colon carcinoma tissue.

AP21118PU-N

Western blot (WB) analysis of MSK1 antibody (Cat.-No.: AP21118PU-N) in extracts from mouse brain cells.

AP21118PU-N

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MSK1 (Phospho-Ser360) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of NIH-3T3 cells, using MSK1 (Phospho-Ser360) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using MSK1 (Phospho-Thr581) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from RAW264.7 cells treated with UV 5', using MSK1 (Phospho-Thr581) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HuvEc cells treated with PMA 125ng/ml 30', using MSK1 (Phospho-Ser376) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MSK1 (Phospho-Ser212) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from NIH-3T3 cells treated with EGF 200ng/ml 5', using MSK1 (Phospho-Ser212) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human pancreas tissue, using MSK1 (Ab-360) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from COS7 cells, treated with Sorbitol 0.4M 30', using MSK1 (Ab-360) Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HuvEc cells, using MSK1 (Ab-581) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells, treated with UV 15', using MSK1 (Ab-581) Antibody. The lane on the right is treated with the synthesized peptide.

Immunofluorescence analysis of A549 cells, using MSK1 (Ab-376) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HT-29 cells, using MSK1 (Ab-376) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MSK1 (Ab-212) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from mouse brain cells, using MSK1 (Ab-212) Antibody. The lane on the right is treated with the synthesized peptide.

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

AM31943PU-N

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

AM31943PU-N

Formalin-Fixed, Paraffin-Embedded Human Heart stained with MSK1 Antibody Cat.-No AP32045PU-N

AP32045PU-N

Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex Neurons stained with MSK1 Antibody Cat.-No AP32045PU-N

AP32045PU-N

Immunofluorescence analysis of HuvEc cells, using MSK1 Antibody Cat.-No AP32045PU-N. The picture on the right is treated with the synthesized peptide.

AP32045PU-N

Western blot analysis of extracts from 293 cells, treated with UV 15', using MSK1 Antibody Cat.-No AP32045PU-N. The lane on the right is treated with the synthesized peptide.

AP32045PU-N

Western blot analysis of MSK1 (pT581) expression in HeLa UV-treated (A); 293 UV-treated (B); SP2/0 UV-treated (C); rat liver (D) whole cell lysates.

Immunohistochemical analysis of MSK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MSK1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of MSK1 expression in HEK293T (A); Raw264.7 (B); PC12 (C) whole cell lysates.

TP300549

TP300549

TP320636

Western blot analysis of MSK1 (pS376) expression in K562 (A); HEK293T (B) whole cell lysates.

Western blot analysis of extracts from mouse brain cells, using MSK1 (Ab-212) antibody.The lane on the right is treated with the synthesized peptide.

TA312503

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using MSK1 (Ab-212) antibody.The picture on the right is treated with the synthesized peptide.

TA312503

Western blot analysis of extracts from Jurkat cells, using SHP-1 (Ab-564) antibody.The lane on the right is treated with the synthesized peptide.

TA312507

Western blot analysis of extracts from JK cells (Lane 2), using SHP-1 (Ab-564) Antibody. The lane on the left is treated with synthesized peptide.

TA312507

Predicted band size: 90 kDa . Positive control: HUVEC cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: Treated with the peptide Lane2: HUVEC cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA322708

Western blot analysis of MSK1 expression in HT29 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325820

Western blot analysis of MSK1 expression in Mouse braintissue lysates,The lane on the left is treated with the antigen-specific peptide.

TA325821

Western blot analysis of MSK1 phosphorylation expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325823

Western blot analysis of MSK1 phosphorylation expression in EGF treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

TA325822

Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 5mins), using MSK1 (Phospho-Ser212) antibody.The lane on the right is treated with the synthesized peptide.

TA312506

Western blot analysis of extracts from colo cells (Lane 2), using MSK1 (Phospho-Ser212) Antibody. The lane on the left is treated with synthesized peptide.

TA312506

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MSK1 (Phospho-Ser212) antibody.The picture on the right is treated with the synthesized peptide.

TA312506

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AP32045PU-N	RPS6KA5 / MSK1 antibody
	Rabbit	IgG		Aff - Purified	Hu, Ms	E, ICC/IF, P, WB	50 µg / €470.00
							Acris Antibodies GmbH
+3 additional images	Highly validated antibody!
TA312503	RPS6KA5 / MSK1 antibody
	Rabbit polyclonal MSK1 (Ab-212) antibody
	Rabbit	IgG		Purified	Hu, Ms	P, WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
+1 additional image
TA312506	RPS6KA5 / MSK1 antibody
	Rabbit polyclonal MSK1 (Ser212) antibody(Phospho-specific)
	Rabbit	IgG		Purified	Hu, Ms	P, WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
+2 additional images
TA312507	RPS6KA5 / MSK1 antibody
	Rabbit polyclonal SHP-1 (Ab-564) antibody
	Rabbit	IgG		Purified	Hu	E, WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
+1 additional image
TA322708	RPS6KA5 / MSK1 antibody
	Anti-RPS6KA5 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
TA325820	RPS6KA5 / MSK1 antibody
	Rabbit Polyclonal MSK1 Antibody
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
TA325821	RPS6KA5 / MSK1 antibody
	Rabbit Polyclonal MSK1 Antibody
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
TA325822	RPS6KA5 / MSK1 antibody
	Rabbit Polyclonal MSK1 (Ser212) Antibody (Phospho-specific)
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
TA325823	RPS6KA5 / MSK1 antibody
	Rabbit Polyclonal MSK1 (Ser376) Antibody (Phospho-specific)
	Rabbit	IgG		Purified	Hu, Ms	WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
AM31943PU-N	RPS6KA5 / MSK1 antibody
	Mouse	IgG2a	1G7	Purified	Hu	E, P	50 µg / €530.00
							Acris Antibodies GmbH
+1 additional image
AP00086PU-N	RPS6KA5 / MSK1 pThr581 antibody
	Rabbit			Aff - Purified	Hu, Ms	WB	0.1 mg / €410.00
							Acris Antibodies GmbH
AP01343PU-N	RPS6KA5 / MSK1 antibody
	Rabbit			Aff - Purified	Hu, Ms	E, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
AP02710PU-N	RPS6KA5 / MSK1 antibody
	Rabbit			Aff - Purified	Hu, Ms	WB	0.1 ml / €320.00
							Acris Antibodies GmbH
AP02710PU-S	RPS6KA5 / MSK1 antibody
	Rabbit			Aff - Purified	Hu, Ms	WB	50 µl / €240.00
							Acris Antibodies GmbH
AP06539PU-N	RPS6KA5 / MSK1 antibody
	Rabbit			Aff - Purified	Hu, Ms	P, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
+1 additional image
AP20352PU-N	RPS6KA5 / MSK1 antibody
	Rabbit			Aff - Purified	Hu, Ms	ICC/IF, P, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
+1 additional image
AP21118PU-N	RPS6KA5 / MSK1 antibody
	Rabbit			Aff - Purified	Hu, Ms	P, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
+1 additional image
A0685-1	RPS6KA5 / MSK1 pSer360 antibody
	Rabbit			Aff - Purified	Hu, Ms	E, ICC/IF, P	50 µg / €230.00
							Assay Biotech
+1 additional image
A0685-2	RPS6KA5 / MSK1 pSer360 antibody
	Rabbit			Aff - Purified	Hu, Ms	E, ICC/IF, P	0.1 mg / €330.00
							Assay Biotech
+1 additional image
A0686-1	RPS6KA5 / MSK1 pThr581 antibody
	Rabbit			Aff - Purified	Hu, Ms	E, ICC/IF, WB	50 µg / €230.00
							Assay Biotech
+1 additional image