RRM1 / RR1 antibody

Principal name

RRM1 / RR1 antibody

Alternative names for RRM1 / RR1 antibody

Ribonucleotide reductase large subunit, Ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase large subunit, Ribonucleoside-diphosphate reductase subunit M1

SwissProt ID

P00452 (Ecoli), P07742 (Mouse), P21524 (Yeast), P23921 (Human), P36602 (Schpo), P48591 (Drome), P79732 (Danre), Q03604 (Caeel), Q9SJ20 (Arath)

Gene ID

6240 (RRM1), 307404 (Arsi), 8624064 (abcA10), 856801 (RNR1)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Xen (Xenopus), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Ce (C. elegans), S. cerevisiae (Saccharomyces cerevisiae)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of RRM1 / RR1 antibody

RRM1 is one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. Its gene is located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. Its gene may play a role in malignancies and disease that involve this region.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1), with the 3\' end of STIM1 situated 1.6 kb from the 5\' end of this gene.

General readings

Bryan,B.A., J. Gen. Virol. 87 (PT 3), 519-529 (2006)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RRM1 / RR1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RRM1 mouse monoclonal antibody. (UM800059; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 UMAB165 Purified Hu P
0.1 ml / €379.00
  OriGene Technologies, Inc.

UltraMAB

RRM1 / RR1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RRM1 mouse monoclonal antibody. (UM800059; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 UMAB165 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

RRM1 / RR1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RRM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801208) Mouse IgG1 OTI5G5 Purified Hu P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RRM1 / RR1 antibody

Human Lung Adenocarcinoma stained with anti-RRM1 Antibody Cat.-No AM32445PU (Clone SP167). Rabbit IgG SP167 Purified Hu F, P
0.5 ml / €520.00
  Acris Antibodies GmbH

RRM1 / RR1 antibody

Human Lung Adenocarcinoma stained with anti-RRM1 Antibody Cat.-No AM32445PU (Clone SP167). Rabbit IgG SP167 Purified Hu F, P
1 ml / €730.00
  Acris Antibodies GmbH

RRM1 / RR1 antibody

Human Lung Adenocarcinoma stained with anti-RRM1 Antibody Cat.-No AM32445PU (Clone SP167). Rabbit IgG SP167 Purified Hu F, P
0.1 ml / €300.00
  Acris Antibodies GmbH

RRM1 / RR1 antibody

IHC Image for Cat# AP15764PU-S / AP15764PU-N Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

RRM1 / RR1 antibody

IHC Image for Cat# AP15764PU-S / AP15764PU-N Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

RRM1 / RR1 antibody

IHC Image for Cat# AP15764PU-S / AP15764PU-N Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

RRM1 / RR1 antibody

Predicted band size: 90 kDa . Positive control: Hela and 231 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: 231 cell lysate Lysates: 50 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RRM1 / RR1 antibody

Predicted band size: 90 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 50 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RRM1 / RR1 antibody

Western blot analysis of A549 cell and SH-SY5Y cell lysate using RRM1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

RRM1 / RR1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RRM1 (RC200726, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RRM1. Mouse IgG1 OTI13E4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RRM1 / RR1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RRM1 (RC200726, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RRM1. Mouse IgG1 OTI3G5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RRM1 / RR1 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RRM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804454) Mouse IgG1 OTI1F9 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RRM1 / RR1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RRM1 (RC200726, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RRM1. Mouse IgG2a OTI12B9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RRM1 / RR1 antibody

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RRM1 Mouse monoclonal antibody. (TA804537; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI16A8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RRM1 / RR1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RRM1 (RC200726, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RRM1. Mouse IgG1 OTI9A2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RRM1 / RR1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RRM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804723) Mouse IgG1 OTI17C3 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RRM1 / RR1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RRM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804875) Mouse IgG1 OTI2D10 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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