RUB1 antibody

Principal name

RUB1 antibody

Alternative names for RUB1 antibody

YDR139C, NEDD8-like protein RUB1, Ubiquitin-like protein RUB1

SwissProt ID

Q03919 (Yeast)

Gene ID

851717 (RUB1)

Available reactivities

Ye (Yeast), S. cerevisiae (Saccharomyces cerevisiae)

Available hosts


Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of RUB1 antibody

Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg12, Hub1. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not proteolytically processed or conjugated to other proteins. Rub1 and the corresponding human homolog Nedd8 are activated by the E1 ubiquitin-activating enzyme UBA2, that forms isopeptide linkages between thio esters. Nedd8 shows 80% homology to ubiquitin. The best known targets of Rub1 modification are members of the cullin family. Cullins are subunits of an E3-ubiquitin ligase complex called the Skp1-/Cul1/Cdc53-F-box (SCF). The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein. Rub1 modification may regulate SCF function or localization.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RUB1 antibody

Figure. Immunoblot of Rub1 fusion protein. Anti-Rub1 antibody, generated by immunization with full-length recombinant yeast Rub1, was tested by immunoblot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4°C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Rabbit Purified Ye E, WB
0.5 mg / €520.00
  Acris Antibodies GmbH

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