RUNX1T1 antibody

Principal name

RUNX1T1 antibody

Alternative names for RUNX1T1 antibody

AML1T1, CBFA2T1, MTG8, ZMYND2, Cyclin-D-related protein, CDR, Eight twenty one protein, ETO, Zinc finger MYND domain-containing protein 2

SwissProt ID

Q06455 (Human), Q61909 (Mouse), Q92479 (Human)

Gene ID

862 (RUNX1T1)

Available reactivities

Hu (Human), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine), Por (Porcine), Eq (Equine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), ChIP assay (ChIP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of RUNX1T1 antibody

RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene.

General readings

Lausen,J., et al., (2004) J. Biol. Chem. 279 (47), 49281-49288

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RUNX1T1 antibody

1Human 721_B; Host: Rabbit; Target Name: WT1; Sample Tissue: 721_B; Antibody Dilution: 1.0 ug/mlRUNX1T1 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RUNX1T1 antibody

WB Suggested Anti-RUNX1T1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysate Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

RUNX1T1 antibody

ChIP assays were performed using SKNO-1 cells, the antibody against ETO and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, NFE2, OGG1 and VEGF genes. Image shows the occupancy, calculated as the ratio + control/background for which the H2B gene was used. Rabbit IgG Purified Hu E
0.1 ml / €325.00
  OriGene Technologies, Inc.

RUNX1T1 antibody

WB was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the ab at 1:1,000. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. C: WB was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the ab against AML-ETO at 1:1,000 . The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated. Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

RUNX1T1 antibody

Anti-RUNX1T1 mouse monoclonal antibody (TA504703) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RUNX1T1(RC222426). Mouse IgG1 OTI1H1 Purified Hu ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RUNX1T1 antibody

ELISA: RUNX1T1 / ETO Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

RUNX1T1 antibody

RUNX1T1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RUNX1T1 antibody

Western Blot analysis of RUNX1T1 expression in transfected 293T cell line (H00000862-T01) by RUNX1T1 MaxPab polyclonal antibody.

Lane 1: RUNX1T1 transfected lysate(63.47 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RUNX1T1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between RUNX1T1 and HDAC1 HeLa cells were stained with anti-RUNX1T1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

RUNX1T1 antibody

Western Blot analysis of RUNX1T1 expression in transfected 293T cell line by RUNX1T1 monoclonal antibody (M01), clone 5A12.

Lane 1: RUNX1T1 transfected lysate(67.566 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 5A12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

RUNX1T1 antibody

Western blot was performed on seven GST-fusion proteins containing different fragments of RUNX1T1 ( Lane 1~7 ).
RUNX1T1 polyclonal antibody ( Cat # PAB14127 ) was used at dilution 1 : 1,000 in TBS-Tween containing 5% skimmed milk. Rabbit Hu E, WB
50 µg / €390.00
  Abnova Taiwan Corp.

RUNX1T1 antibody

Human 721_B; Host: Rabbit. Target Name: WT1 . Sample Tissue: 721_B . Antibody Dilution: 1.0ug/ml. RUNX1T1 is supported by BioGPS gene expression data to be expressed in 721_B.; RUNX1T1 antibody - middle region (ARP33996_T100) in Human 721_B cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

RUNX1T1 antibody

Human 293T; WB Suggested Anti-RUNX1T1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; RUNX1T1 antibody - middle region (ARP58127_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

RUNX1T1 antibody

ELISA: RUNX1T1 / ETO Antibody [37450002] Rabbit Hu E, WB
50 µg / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RUNX1T1 (transcript variant 4)

RUNX1T1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

RUNX1T1 (transcript variant 3)

RUNX1T1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

RUNX1T1

RUNX1T1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RUNX1T1

RUNX1T1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

RUNX1T1

RUNX1T1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

RUNX1T1

RUNX1T1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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