RUNX3 antibody

Principal name

RUNX3 antibody

Alternative names for RUNX3 antibody

Runt-related transcription factor 3, AML2, CBFA3, CBF-alpha-3, PEBP2A3, PEBP2-alpha C, PEA2-alpha C, Core-binding factor subunit alpha-3, Acute myeloid leukemia 2 protein, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit

SwissProt ID

Q13761 (Human), Q64131 (Mouse)

Gene ID

864 (RUNX3)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Chk (Chicken), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of RUNX3 antibody

The mammalian Runt-related transcription factor (RUNX) family comprises three members, RUNX1 (also designated AML-1, PEBP2αB, CBFA2), RUNX2 (also designated AML-3, PEBP2αA, CBFA1, Osf2) and RUNX3 (also designated AML-2, PEBPαC, CBFA3), and belongs to the acute myeloid leukemia (AML) family. RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX3 is expressed in cells of hematopoietic origin, including myeloid and B-cell lines and spleen. By playing a role in controlling the growth and differentiation of gastric epithelial cells, RUNX3 is a strong candidate as a gastric cancer tumor suppressor. Specifically, hypermethylation inactivates the gene encoding RUNX3. The detection of hypermethylation at multiple regions within the RUNX3 CpG island may aid in the diagnosis and risk assessment of gastric cancer.

General readings

Vogiatzi P. et al. (2006) Cancer Biol Ther. 5(4):371-4.
Li QL,et al. (2002)Cell. 109(1):113-24.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RUNX3 antibody

Immunoperoxidase of purified MaxPab antibody to RUNX3 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml] Mouse Serum Hu E(detection), ICC/IF, P, WB
50 µl / €370.00
  Abnova Taiwan Corp.

RUNX3 antibody

Immunoperoxidase of purified MaxPab antibody to RUNX3 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml] Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RUNX3 antibody

Immunofluorescent: Runx3 Antibody [NB100-91276] -  Runx3 Antibody [NB100-91276] - mouse cerebellum (4% PFA fixed, frozen section) at 1:500 dilution using internal part of Runx3. DAPI counterstained appearing in blue. Rabbit Serum Ms, Rt C, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

RUNX3 antibody

Western blot analysis of extracts from 293/HeLa cells, using RUNX3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RUNX3 antibody

Predicted band size: 44kDa. Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RUNX3 antibody

Predicted band size: 44kDa. Positive control: Mouse thymus tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

RUNX3 (Center) antibody

Western blot analysis of lysates from HL-60,  Jurkat cell line (from left to right),  using RUNX3 Antibody (Center) (Cat.   #TA324560).  TA324560 was diluted at 1:1000 at each lane.  A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.  Lysates at 35ug per lane. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

RUNX3 antibody

WB Suggested Anti-RUNX3 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:12500; Positive Control: NIH/3T3 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RUNX3 antibody

Western blot analysis of RUNX3 antibody (Cat.-No.: AP20638PU-N) in extracts from HUVEC cells. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €350.00
  Acris Antibodies GmbH

RUNX3 antibody

RUNX3 polyclonal antibody (A01), Lot # 070226JCSa Western Blot analysis of RUNX3 expression in HepG2 ( Cat # L019V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

RUNX3 antibody

Immunoprecipitation of RUNX3 transfected lysate using anti-RUNX3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RUNX3 MaxPab mouse polyclonal antibody (B01) (H00000864-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

RUNX3 antibody

Mouse NIH-3T3; WB Suggested Anti-RUNX3 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: NIH/3T3 cell lysate; RUNX3 antibody - C-terminal region (ARP37263_T100) in Mouse NIH-3T3 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

RUNX3 antibody

RUNX3 Rabbit IgG Aff - Purified Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

RUNX3 antibody

Western blot analysis of RUNX3 expression in Jurkat (A); Raji (B); SW480 (C) whole cell lysates. Rabbit Aff - Purified Chk, Hu WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

RUNX3 antibody

Western blot analysis of RUNX3 expression in Jurkat (A); Raji (B); SW480 (C) whole cell lysates. Rabbit Aff - Purified Chk, Hu WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

RUNX3 antibody

Western blot analysis of RUNX3 expression in Jurkat (A); Raji (B); SW480 (C) whole cell lysates. Rabbit Aff - Purified Chk, Hu WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

RUNX3 antibody

Detection of AML-2 by EMSA analysis. Nushift polyclonal antibody against AML-2 factor creates a supershift in EMSA (lane 4). Rabbit IgG Serum Hu C, P, WB
50 µl / €320.00
  Novus Biologicals Inc.

RUNX3 antibody

RUNX3 Sheep Serum Ms, Rt P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

RUNX3 antibody

Immunohistochemistry-Paraffin: RUNX3 Antibody [NBP1-46694] - IHC on rat brain (paraffin section) using Rabbit antibody to c-terminal region of Runt related transcription factor 3 (Runx3): whole serum at 1 : 300 dilution incubated overnight at 4C. Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RUNX3 (full length, N-term HIS tag, transcript variant 1)

RUNX3 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

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