ruvA antibody

Principal name

ruvA antibody

Alternative names for ruvA antibody

Holliday junction ATP-dependent DNA helicase ruvA

SwissProt ID

P0A809 (Ecoli)

Gene ID

946369 (ruvA)

Available reactivities

E. coli (Escherichia coli), Bac (Bacteria)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB)

Background of ruvA antibody

In Escherichia coli, the RuvA, RuvB and RuvC proteins are required for the late stages of homologous recombination and DNA repair. They are involved in processing the Holliday junction during homologous recombination. RuvA protein binds to both single-stranded and double-stranded DNA. RuvB protein has weak ATPase activity. RuvA bound to DNA greatly enhances ATPase activity of RuvB. UV-irradiation to supercoiled DNA further enhances the stimulatory effect of RuvA on the RuvB ATPase activity. In the presence of ATP the RuvA-RuvB complex has an activity that renatures cruciform structures formed by heating and gradually cooling supercoiled DNA with an inverted repeat. RuvA and RuvB promote branch migration whereas RuvC resolves junctions by endonucleolytic cleavage. Moreover RuvAB stimulate Holliday junction resolution by RuvC. The RuvA-RuvB complex interacts with an irregular conformation in damaged DNA and induces conformational changes in DNA using energy provided by ATP hydrolysis, so that it facilitates DNA repair, recombination and error prone replication. RuvABC proteins are responsible for the occurrence of DSBs at arrested replication forks. In cells proficient for RecBC, RuvAB is uncoupled from RuvC and DSBs may be prevented.

General readings

This product has been used in:Davies AA & West SC Formation of RuvABC-Holliday junction complexes in vitro. Curr Biol 8:725-7 (1998). PubMed PMID: 9637927

3 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ruvA antibody

ruvA Rabbit IgG Serum Bac IP, WB
0.2 mg / €320.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ruvA

ruvA E. coli
20 µg / €520.00
  Funakoshi Co., Ltd.

ruvA

ruvA E. coli
0.1 mg / €1,460.00
  Funakoshi Co., Ltd.

3 Item(s)

per page
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