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RXR-beta / RXRB antibody

Principal name

RXR-beta / RXRB antibody

Alternative names for RXR-beta / RXRB antibody

NR2B2, H-2RIIBP, Rcor-1, Retinoic acid receptor RXR-beta, Retinoid X receptor beta, Nuclear receptor subfamily 2 group B member 2, Nuclear Receptor Coregulator 1

SwissProt ID

P28702 (Human), P28704 (Mouse), P49743 (Rat), Q5TJF7 (Canfa)

Gene ID

6257 (RXRB), 20182 (Rxrb), 361801

Ncbi ID

NP_068811, NP_035436, 9606, NP_068811.1, NP_001192145.1

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Electrophoretic Mobility Shift Assay (EMSA), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Paraffin Sections (P), Dot blot (Dot)

Background of RXR-beta / RXRB antibody

RXRB is a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid (RA). This receptor forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid (RA). This receptor forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. The gene lies within the major histocompatibility complex (MHC) class II region on chromosome 6. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.This gene encodes a member of the retinoid X receptor (RXR) family of nuclear receptors which are involved in mediating the effects of retinoic acid (RA). This receptor forms homodimers with the retinoic acid, thyroid hormone, and vitamin D receptors, increasing both DNA binding and transcriptional function on their respective response elements. The gene lies within the major histocompatibility complex (MHC) class II region on chromosome 6. An alternatively spliced transcript variant has been described, but its full length sequence has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

This product has been used in:Kephart DD et al. Retinoid X receptor isotype identity directs human vitamin D receptor heterodimer transactivation from the 24-hydroxylase vitamin D response elements in yeast. Mol Endocrinol 10:408-19 (1996). PubMed PMID: 8721985
Holley SJ & Yamamoto KR A role for Hsp90 in retinoid receptor signal transduction. Mol Biol Cell 6:1833-42 (1995). PubMed PMID: 8590809
Segars JH et al. Inhibition of estrogen-responsive gene activation by the retinoid X receptor beta: evidence for multiple inhibitory pathways. Mol Cell Biol 13:2258-68 (1993). PubMed PMID: 8384307
MacDonald PN et al. Retinoid X receptors stimulate and 9-cis retinoic acid inhibits 1,25-dihydroxyvitamin D3-activated expression of the rat osteocalcin gene. Mol Cell Biol 13:5907-17 (1993). PubMed PMID: 8395017
Hallenbeck PL et al. Heterodimerization of thyroid hormone (TH) receptor with H-2RIIBP (RXR beta) enhances DNA binding and TH-dependent transcriptional activation. Proc Natl Acad Sci U S A 89:5572-6 (1992). PubMed PMID: 1608968
Marks MS et al. H-2RIIBP expressed from a baculovirus vector binds to multiple hormone response elements. Mol Endocrinol 6:219-30 (1992). PubMed PMID: 1569965
Mangelsdorf DJ et al. A direct repeat in the cellular retinol-binding protein type II gene confers differential regulation by RXR and RAR. Cell 66:555-61 (1991). PubMed PMID: 1651173
General / background references:Minucci S et al. Dominant negative retinoid X receptor beta inhibits retinoic acid-responsive gene regulation in embryonal carcinoma cells. Mol Cell Biol 14:360-72 (1994). PubMed PMID: 8264603
Berrodin TJ et al. Heterodimerization among thyroid hormone receptor, retinoic acid receptor, retinoid X receptor, chicken ovalbumin upstream promoter transcription factor, and an endogenous liver protein. Mol Endocrinol 6:1468-78 (1992). PubMed PMID: 1331778

Western Blot analysis of RXRB expression in transfected 293T cell line (H00006257-T01) by RXRB MaxPab polyclonal antibody.Lane 1: RXRB transfected lysate(58.63 KDa).Lane 2: Non-transfected lysate.

H00006257-B01

NB 100-1467 staining (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

NB100-1467

Western Blot: Retinoid X Receptor beta Antibody [NB100-1467] - HEK293 overexpressing RXRB (RC200516) and probed (mock transfection in first lane), tested by Origene.

NB100-1467

RXRB polyclonal antibody ( Cat # PAB6693 ) staining ( 1 µg/ml ) of A-431 lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

PAB6693

HEK293 overexpressing RXRB and probed with RXRB polyclonal antibody ( Cat # PAB6693 ) ( mock transfection in first lane ), tested by Origene.

PAB6693

Western Blot analysis of RXRB expression in transfected 293T cell line by RXRB monoclonal antibody (M01), clone 3C8.Lane 1: RXRB transfected lysate(56.9 KDa).Lane 2: Non-transfected lysate.

H00006257-M01

Human Placenta; WB Suggested Anti-RXRB Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Placenta; RXRB antibody - C-terminal region (ARP39524_P050) in Human Placenta cells using Western Blot

ARP39524_P050

Human Brain; WB Suggested Anti-RXRB Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; RXRB antibody - N-terminal region (ARP45637_P050) in Human Brain cells using Western Blot

ARP45637_P050

Hum. Adult Placenta; Host:Rabbit. Target Name:RXRB. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; RXRB antibody - N-terminal region (ARP45637_P050) in Hum. Adult Placenta cells using Western Blot

ARP45637_P050

Hum. Fetal Muscle; Host:Rabbit. Target Name:RXRB. Sample Tissue:Human Fetal Muscle. Antibody Dilution: 1.0ug/ml.; RXRB antibody - N-terminal region (ARP45637_P050) in Hum. Fetal Muscle cells using Western Blot

ARP45637_P050

Human Jurkat; WB Suggested Anti-RXRB Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. RXRB is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; RXRB antibody - N-terminal region (ARP45638_P050) in Human Jurkat cells using Western Blot

ARP45638_P050

HEK293 overexpressing RXRB and probed with AP16283PU-N (mock transfection in first lane), tested by Origene.

AP16283PU-N

AP16283PU-N staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

AP16283PU-N

Western blot of hippocampal lysate showing specific immunolabeling of the ~48k RXRB protein. Using RXRB monoclonal antibody, clone 147 ( Cat # MAB1788 ).

MAB1788

Figure 1. Western blot of hippocampal lysate showing specific immunolabeling of the ~48k RXR-beta protein.

AM08264PU-N

NBL1-15642 - Retinoid X Receptor beta Lysate

Western Blot: Retinoid X Receptor beta Lysate [NBL1-15642] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for RXRB

NBL1-15642

Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 7.5% SDS PAGE GTX104798 diluted at 1:1000

GTX104798

Retinoid X Receptor beta antibody detects RXRB protein at nucleus and cytosol on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. Retinoid X Receptor beta antibody (GTX113544) dilution: 1:500.

GTX113544

Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX113544 diluted at 1:1000

GTX113544

Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GTX113544 diluted at 1:2000

GTX113544

Western Blot: Retinoid X Receptor beta Antibody [NBP1-30897] - Whole cell lysate treated with Retinoid X Receptor beta Antibody, diluted at 1:1000 with 7.5% SDS PAGE gel.

NBP1-30897

Western Blot: Retinoid X Receptor beta Antibody [NBP1-33543] – Western blot analysis of Retinoid X Receptor beta on Molt-4 lysates using NBP1-33543 at a dilution of 1:1000. 30ug whole cell lysate loaded on 7.5% SDS PAGE gel.

NBP1-33543

Human HEK293T; WB Suggested Anti-RXRB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T. RXRB is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; RXRB antibody - N-terminal region (P100796_P050) in Human HEK293T cells using Western Blot

P100796_P050

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14684-1-AP(RXRB antibody) at dilution of 1:500

14684-1-AP

MCF7 cells were subjected to SDS PAGE followed by western blot with 14684-1-AP(RXRB antibody) at dilution of 1:500

14684-1-AP

IP Result of anti-RXRB (IP:14684-1-AP, 4ug; Detection:14684-1-AP 1:500) with MCF-7 cells lysate 1200ug.

14684-1-AP

Western Blot: Retinoid X Receptor beta Antibody [NBP1-52812] - This Anti-RXRB antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 1ug/ml.

NBP1-52812

Western blot analysis of RXRB Antibody (Center) Cat.-No AP53774PU-N in MDA-MB231 cell line lysates (35 µg/lane). RXRB (arrow) was detected using the purified Pab.

AP53774PU-N

Western blot of hippocampal lysate showing specific immunolabeling of the ~48k RAR-beta protein using GTX82600

GTX82600

GTX89670 staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

GTX89670

HEK293 overexpressing RXRB (RC200516) and probed with GTX89670 (mock transfection in first lane)

GTX89670

TA302594 staining (1µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

TA302594

Sample (30 ug of whole cell lysate). A: A431 . 7.5% SDS PAGE. TA308585 diluted at 1:1000.

TA308585

Western blot of hippocampal lysate showing specific immunolabeling of the ~48k RXR-Î² protein.

TA309231

WB Suggested Anti-RXRB Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293TRXRB is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells

TA330072

WB Suggested Anti-RXRB Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain

TA338001

Host: Rabbit; Target Name: RXRB; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml

TA338001

Host: Rabbit; Target Name: RXRB; Sample Tissue: Human Fetal Muscle; Antibody Dilution: 1.0 ug/ml

TA338001

WB Suggested Anti-RXRB Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate. RXRB is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells

TA338002

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA302594	RXR-beta / RXRB antibody
	Goat			Aff - Purified	Hu, Ms, Rt	WB	0.1 mg / €325.00
							OriGene Technologies, Inc.
TA308585	RXR-beta / RXRB antibody
	Rabbit	IgG		Aff - Purified	Hu	WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
TA309231	RXR-beta / RXRB (Hinge Region) antibody
	Mouse	IgG1	147	Aff - Purified	Hu, Ms, Rt	WB	0.1 ml / €325.00
							OriGene Technologies, Inc.
TA330072	RXR-beta / RXRB antibody
	Rabbit	IgG		Purified	Hu	WB	50 µg / €325.00
							OriGene Technologies, Inc.
TA338001	RXR-beta / RXRB antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, GP, Hu, Ms, Rb, Rt	WB	50 µg / €325.00
							OriGene Technologies, Inc.
+2 additional images
TA338002	RXR-beta / RXRB antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt	WB	50 µg / €325.00
							OriGene Technologies, Inc.
TA345349	RXR-beta / RXRB antibody
	Rabbit	IgG		Purified	Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze	WB	50 µg / €325.00
							OriGene Technologies, Inc.
AP53774PU-N	RXR-beta / RXRB (Center) antibody
	Rabbit	Ig		Aff - Purified	Hu	WB	0.4 ml / €370.00
							Acris Antibodies GmbH
H00006257-B01	RXR-beta / RXRB antibody
	Mouse			Serum	Hu	E(detection), WB	50 µl / €370.00
							Abnova Taiwan Corp.
H00006257-M01	RXR-beta / RXRB antibody
	Mouse	IgG2b	3C8	Purified	Hu	E, WB	0.1 mg / €370.00
							Abnova Taiwan Corp.
H00006257-M10	RXR-beta / RXRB antibody
	Mouse	IgG1	2E6	Purified	Hu	E, WB	0.1 mg / €370.00
							Abnova Taiwan Corp.
H00006257-M11	RXR-beta / RXRB antibody
	Mouse	IgG1	2G3	Purified	Hu	E, WB	0.1 mg / €370.00
							Abnova Taiwan Corp.
H00006257-M12	RXR-beta / RXRB antibody
	Mouse	IgG2b	1H1	Purified	Hu	E, WB	0.1 mg / €370.00
							Abnova Taiwan Corp.
MAB1788	RXR-beta / RXRB antibody
	Mouse	IgG1	147		Hu, Ms, Rt		0.1 ml / €470.00
							Abnova Taiwan Corp.
PAB6693	RXR-beta / RXRB antibody
	Goat				Hu	E, WB	0.1 mg / €400.00
							Abnova Taiwan Corp.
+1 additional image
ARP39524_P050	RXR-beta / RXRB antibody
	Rabbit			Aff - Purified	Bov, Can, Chk, Hu, Ms, Por, Rt	WB	50 µg / €350.00
							AVIVA Systems Biology
ARP45637_P050	RXR-beta / RXRB antibody
	Rabbit			Aff - Purified	Bov, Can, Hu, Ms, Por, Rt	WB	50 µg / €350.00
							AVIVA Systems Biology
+2 additional images
ARP45638_P050	RXR-beta / RXRB antibody
	Rabbit			Aff - Purified	Bov, Can, Hu, Ms, Por, Rt	WB	50 µg / €350.00
							AVIVA Systems Biology
P100796_P050	RXR-beta / RXRB antibody
	Rabbit			Aff - Purified	Bov, Can, Hu, Ms, Por, Rt	WB	50 µg / €350.00
							AVIVA Systems Biology
F-PP-H7341-00	RXR-beta / RXRB antibody
	Mouse	IgG2a	H7341	Purified	Hu, Rt	ELISA, WB	0.1 ml / €640.00
							Funakoshi Co., Ltd.

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