RYBP antibody

Principal name

RYBP antibody

Alternative names for RYBP antibody

RING1 and YY1-binding protein, DEDAF, YEAF1, AAP-1, APAP-1, DED-associated factor, YY1 and E4TF1-associated factor 1, Death effector domain-associated factor, Apoptin-associating protein 1

SwissProt ID

Q8CCI5 (Mouse), Q8N488 (Human)

Gene ID

23429 (RYBP)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine), Chk (Chicken), Can (Canine), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F)

Background of RYBP antibody

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor . DEDAF is identical to the transcriptional repressor RYBP (2). DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.

General readings

Chen D, et al. (2008) EMBO Rep. 10(2):166-72. Zheng L., et al. (2001) J. Biol. Chem. 276:31945-31952

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RYBP antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RYBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504650) Mouse IgG1 OTI1B2 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 ug /ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RYBP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI2F6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI2B4 Purified Can, Hu, Mky, Ms WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI2D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI7D1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI5A9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

Anti-RYBP mouse monoclonal antibody (TA504761) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RYBP(RC206186). Mouse IgG1 OTI4E10 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI1B6 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI2H9 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

Western blot analysis of DEDAF expression in HepG2 cell lysate. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

RYBP (215-228) antibody

Figure 2. Formalin-Fixed Paraffin-Embedded (FFPE) on Spleen. Rabbit Aff - Purified Bear, Bov, Can, Chk, Elephant, Eq, Hst, Hu, L, Mky, Ms, Op, Platypus, Prim, Rb, Rt, Tr, Xen P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RYBP Control Peptide

RYBP
50 µg / €160.00
  Acris Antibodies GmbH

RYBP (N-term) antibody

Western blot analysis in A375 cell line lysates (35 ug/lane) using RYBP Antibody (N-term) Cat.-No. AP53775PU-N. This demonstrates the RYBP antibody detected the RYBP protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

RYBP antibody

Immunofluorescence of purified MaxPab antibody to RYBP on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

RYBP antibody

Immunofluorescence of purified MaxPab antibody to RYBP on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

RYBP antibody

Western Blot analysis of RYBP expression in transfected 293T cell line (H00023429-T02) by RYBP MaxPab polyclonal antibody.

Lane 1: RYBP transfected lysate(25.08 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

RYBP antibody

RYBP Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

RYBP antibody

Immunoprecipitation of RYBP transfected lysate using anti-RYBP MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RYBP MaxPab mouse polyclonal antibody (B02) (H00023429-B02). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

RYBP antibody

Immunoprecipitation of RYBP transfected lysate using anti-RYBP MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with RYBP MaxPab mouse polyclonal antibody (B01) (H00023429-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

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