RYBP antibody

Principal name

RYBP antibody

Alternative names for RYBP antibody

RING1 and YY1-binding protein, DEDAF, YEAF1, AAP-1, APAP-1, DED-associated factor, YY1 and E4TF1-associated factor 1, Death effector domain-associated factor, Apoptin-associating protein 1

SwissProt ID

Q8CCI5 (Mouse), Q8N488 (Human)

Gene ID

23429 (RYBP), 56353 (Rybp)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Hst (Hamster), Mky (Monkey), Op (Opossum), Prim (Primate), Rb (Rabbit), Xen (Xenopus), Tr (Turkey), L (Lizard), Bear, Ze (Zebrafish), African clawed frog

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Flow Cytometry (F)

Background of RYBP antibody

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor . DEDAF is identical to the transcriptional repressor RYBP (2). DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.

General readings

Chen D, et al. (2008) EMBO Rep. 10(2):166-72. Zheng L., et al. (2001) J. Biol. Chem. 276:31945-31952

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

RYBP antibody

  RYBP mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RYBP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504650) Mouse IgG1 OTI1B2 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

  Rabbit Polyclonal DEDAF Antibody  
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates with DEDAF antibody at 1 ug /ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

RYBP antibody

  RYBP mouse monoclonal antibody, clone OTI2F6 (formerly 2F6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI2F6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

  RYBP mouse monoclonal antibody, clone OTI2B4 (formerly 2B4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI2B4 Purified Can, Hu, Mky, Ms WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

  RYBP mouse monoclonal antibody, clone OTI2D4 (formerly 2D4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI2D4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

  RYBP mouse monoclonal antibody, clone OTI7D1 (formerly 7D1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI7D1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

  RYBP mouse monoclonal antibody, clone OTI5A9 (formerly 5A9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI5A9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

  RYBP mouse monoclonal antibody, clone OTI4E10 (formerly 4E10)  
Anti-RYBP mouse monoclonal antibody (TA504761) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RYBP(RC206186). Mouse IgG1 OTI4E10 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

  RYBP mouse monoclonal antibody, clone OTI1B6 (formerly 1B6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI1B6 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

  RYBP mouse monoclonal antibody, clone OTI2H9 (formerly 2H9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RYBP (RC206186, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RYBP. Mouse IgG1 OTI2H9 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

RYBP antibody

Western blot analysis of DEDAF expression in HepG2 cell lysate. Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €390.00
  Acris Antibodies GmbH

RYBP (215-228) antibody

Figure 2. Formalin-Fixed Paraffin-Embedded (FFPE) on Spleen. Rabbit Aff - Purified Bear, Bov, Can, Chk, Elephant, Eq, Hst, Hu, L, Mky, Ms, Op, Platypus, Prim, Rb, Rt, Tr, Xen P, WB
50 µg / €470.00
  Acris Antibodies GmbH

RYBP (N-term) antibody

Western blot analysis in A375 cell line lysates (35 ug/lane) using RYBP Antibody (N-term) Cat.-No. AP53775PU-N. This demonstrates the RYBP antibody detected the RYBP protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Rybp - mouse gene knockout kit via CRISPR

Rybp - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Rybp - mouse gene knockout kit via CRISPR

Rybp - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rybp - mouse gene knockout kit via CRISPR

Rybp - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Rybp - mouse gene knockout kit via CRISPR

Rybp - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

RYBP

  Recombinant protein of human RING1 and YY1 binding protein (RYBP)  
RYBP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

RYBP (1-228, His-tag)

RYBP Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

RYBP (1-228, His-tag)

RYBP Human Purified > 85 % by SDS - PAGE E. coli
20 µg / €300.00
  Acris Antibodies GmbH

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