S-arrestin / SAG antibody

Principal name

S-arrestin / SAG antibody

Alternative names for S-arrestin / SAG antibody

Retinal S-antigen, Rod photoreceptor arrestin, S-AG

SwissProt ID

P08168 (Bovin), P10523 (Human), P15887 (Rat), P20443 (Mouse), P79260 (Pig), Q28281 (Canfa)

Gene ID

25539 (Sag), 397151 (SAG), 6295

Available reactivities

Bov (Bovine), Rt (Rat), Hu (Human), Por (Porcine), Gt (Goat), Sh (Sheep), Ms (Mouse)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Neutralisation (NEUT), Immunoprecipitation (IP), Enzyme Immunoassay (E)

Background of S-arrestin / SAG antibody

Arrestin proteins are a family of regulators of cell sigling of G protein-coupled receptors (GPCR). S-arrestin was first discovered as a result of the experimental model of human uveitis, an autoimmune disease of the eye. In this model, called experimental allergic uveitis, animals were injected with extracts made from the reti of the same species mixed with Freund's complete adjuvant. The animals mounted a strong immune response to the extract, and the antibody response was used to identify several immunogenic retil proteins. One of these was called S-antigen, for soluble antigen. The protein was found to be abundant in reti, about 48 kDa in molecular weight, and localized in the outer segments of the photoreceptors. Several years later, Hermann Kuhn and colleagues discovered that this protein binds to phosphorylated Rhodopsin and prevents this protein from activating transducin. Transducin is a typical heterotrimeric G protein, composed of alpha and beta gamma subunits. Rhodopsin phosphorylation is mediated by Rhodopsin kise (a.k.a. GRK1), the prototypic member of a family of GPCR kises. Since the S-antigen protein arrested the activity of Rhodopsin it was remed S-arrestin, and became the prototypic member of the arrestin protein family. Subsequently, Robert Lefkowitz and colleagues discovered a related protein which bound to phosphorylated beta-adrenergic GPCRs and prevented these proteins from activating their specific heterotrimeric G proteins. Because of this relationship to the beta-adrenergic receptor and functiol and structural similarities to S-arrestin this protein was med beta-arrestin. The beta-adrenergic receptor was phosphorylated by the beta-adrenergic receptor kise (a.k.a. GRK2), an enzyme belonging to GPCR kise family. Studies of visual transduction therefore aided greatly in understanding other kinds of GPCR sigling. In mammals, there are four arrestin isoforms; S-arrestin (a.k.a. S-antigen, visual arrestin and arrestin-1) and cone arrestin (a.k.a. arrestin-4) are largely confined to photoreceptors. Beta-arrestin 1 (a.k.a. arrestin 2) and beta-arrestin-2 (a.k.a. arrestin-3) are ubiquitous and regulate non-visual GPCRs.

General readings

1. J. Biol. Chem., 276(35):32648-56, 2001.
2. Methods Enzymol., 343:600-11, 2002.
3. J. Biol. Chem., 276(35):32648-56, 2001.
IOVS 46(1):12-16, 2005.

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

S-arrestin / SAG antibody

Western Blot: Visual Arrestin Antibody (PDS-1) - Visual Arrestin (PDS-1): anti-PDS-1. Western blot analysis of Visual Arrestin expression in human retina tissue extract. Mouse IgG1 PDS-1 Purified Bov, Hu, Por C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

S-arrestin / SAG antibody

Western Blot: S-arrestin Antibody (S128) [TA337114] - Blot of bovine retinal extracts. The antibody stains a band corresponing to S-arrestin at about 48 kDa. Mouse IgG1 S128 Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

S-arrestin / SAG antibody

S-arrestin / SAG Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

S-arrestin / SAG antibody

Western blot analysis of SAG monoclonal antibody, clone PDS-1 ( Cat # MAB2318 ) in human retina tissue extract. Mouse IgG1 PDS-1 Bov, Hu C, ICC/IF, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

S-arrestin / SAG antibody

S-arrestin / SAG Mouse IgG1 PDS-1 Biotin Bov, Hu, Por C, ICC/IF, P, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

S-arrestin / SAG antibody

S-arrestin / SAG Mouse IgG1 PDS-1 DyLight488 Bov, Hu, Por C, ICC/IF, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

S-arrestin / SAG antibody

S-arrestin / SAG Mouse IgG1 PDS-1 HRP Bov, Hu, Por C, ICC/IF, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

S-arrestin / SAG antibody

S-arrestin / SAG NEUT
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

S-arrestin / SAG

S-arrestin / SAG Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SAG overexpression lysate

SAG overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

10 Item(s)

per page
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