S-arrestin / SAG antibody

Principal name

S-arrestin / SAG antibody

Alternative names for S-arrestin / SAG antibody

Retinal S-antigen, Rod photoreceptor arrestin, S-AG

SwissProt ID

P08168 (Bovin), P10523 (Human), P15887 (Rat), P20443 (Mouse), P79260 (Pig), Q28281 (Canfa)

Gene ID

397151 (SAG), 6295, 20215 (Sag)

Available reactivities

Bov (Bovine), Hu (Human), Por (Porcine), Ms (Mouse), Rt (Rat)

Available hosts

Mouse

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP)

Background of S-arrestin / SAG antibody

Arrestin proteins are a family of regulators of cell sigling of G protein-coupled receptors (GPCR). S-arrestin was first discovered as a result of the experimental model of human uveitis, an autoimmune disease of the eye. In this model, called experimental allergic uveitis, animals were injected with extracts made from the reti of the same species mixed with Freund's complete adjuvant. The animals mounted a strong immune response to the extract, and the antibody response was used to identify several immunogenic retil proteins. One of these was called S-antigen, for soluble antigen. The protein was found to be abundant in reti, about 48 kDa in molecular weight, and localized in the outer segments of the photoreceptors. Several years later, Hermann Kuhn and colleagues discovered that this protein binds to phosphorylated Rhodopsin and prevents this protein from activating transducin. Transducin is a typical heterotrimeric G protein, composed of alpha and beta gamma subunits. Rhodopsin phosphorylation is mediated by Rhodopsin kise (a.k.a. GRK1), the prototypic member of a family of GPCR kises. Since the S-antigen protein arrested the activity of Rhodopsin it was remed S-arrestin, and became the prototypic member of the arrestin protein family. Subsequently, Robert Lefkowitz and colleagues discovered a related protein which bound to phosphorylated beta-adrenergic GPCRs and prevented these proteins from activating their specific heterotrimeric G proteins. Because of this relationship to the beta-adrenergic receptor and functiol and structural similarities to S-arrestin this protein was med beta-arrestin. The beta-adrenergic receptor was phosphorylated by the beta-adrenergic receptor kise (a.k.a. GRK2), an enzyme belonging to GPCR kise family. Studies of visual transduction therefore aided greatly in understanding other kinds of GPCR sigling. In mammals, there are four arrestin isoforms; S-arrestin (a.k.a. S-antigen, visual arrestin and arrestin-1) and cone arrestin (a.k.a. arrestin-4) are largely confined to photoreceptors. Beta-arrestin 1 (a.k.a. arrestin 2) and beta-arrestin-2 (a.k.a. arrestin-3) are ubiquitous and regulate non-visual GPCRs.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

S-arrestin / SAG antibody

  Mouse Monoclonal S-arrestin Antibody (PDS-1)  
Western Blot: Visual Arrestin Antibody (PDS-1) - Visual Arrestin (PDS-1): anti-PDS-1. Western blot analysis of Visual Arrestin expression in human retina tissue extract. Mouse IgG1 PDS-1 Purified Bov, Hu, Por C, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

S-arrestin / SAG antibody

  Mouse Monoclonal S-arrestin Antibody (S128)  
Western Blot: S-arrestin Antibody (S128) [TA337114] - Blot of bovine retinal extracts. The antibody stains a band corresponing to S-arrestin at about 48 kDa. Mouse IgG1 S128 Purified Bov, Hu, Ms, Por, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

S-arrestin / SAG antibody

  Mouse Monoclonal anti-ß-Arrestin-1 Antibody  
NIH/3T3 (lane 1) and Hela (lane 2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with beta;-Arrestin-1 (25G10).  Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse 25-G10 Purified Bov, Hu, Ms, Rt IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

S-arrestin / SAG antibody

  Mouse Monoclonal anti-ß-Arrestin-1/2 Antibody  
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-beta arrestin-1/2 antibody (clone 21-B1). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Mouse 21-B1 Purified Hu IP, WB
0.1 mg / €248.00
  OriGene Technologies, Inc.

S-arrestin / SAG antibody

S-arrestin / SAG Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

S-arrestin / SAG antibody

Western blot analysis of SAG monoclonal antibody, clone PDS-1 ( Cat # MAB2318 ) in human retina tissue extract. Mouse IgG1 PDS-1 Bov, Hu C, ICC/IF, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

CRISPR

Catalog No. Species Donor Vector  

SAG - human gene knockout kit via CRISPR

SAG - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SAG - human gene knockout kit via CRISPR

SAG - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SAG - human gene knockout kit via CRISPR

SAG - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SAG - human gene knockout kit via CRISPR

SAG - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sag - mouse gene knockout kit via CRISPR

Sag - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Sag - mouse gene knockout kit via CRISPR

Sag - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sag - mouse gene knockout kit via CRISPR

Sag - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sag - mouse gene knockout kit via CRISPR

Sag - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

S-arrestin / SAG

  Recombinant protein of human S-antigen; retina and pineal gland (arrestin) (SAG)  
S-arrestin / SAG Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SAG overexpression lysate

SAG overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

16 Item(s)

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