S100A4 antibody

Principal name

S100A4 antibody

Alternative names for S100A4 antibody

S100-A4, CAPL, MTS1, Calvasculin, Metastasin, FSP-1, FSP1, Fibroblast marker, Fibroblast specific protein-1, S100 Calcium-Binding Protein A4, Placental Calcium-Binding Protein

SwissProt ID

P05942 (Rat), P07091 (Mouse), P26447 (Human), P35466 (Bovin), Q9TV56 (Canfa)

Gene ID

6275 (S100A4), 20198 (S100a4), 24615

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Fe (Feline)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), ELISA (capture) (E(capture)), Flow Cytometry (F), Frozen Sections (C)

Background of S100A4 antibody

S100 calcium binding protein A4 (S100A4) is a member of the S100 family of calcium-binding proteins that contain two Ca(2+)-binding sites including a canonical EF-hand motif. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells. S100A4 interacts with cytoskeletal proteins and enhances metastasis of several types of cancer cells. It is secreted by unknown mechanisms, thus, paracrinely stimulating a variety of cellular responses, including angiogenesis and neuronal growth (1). S100A4 has been shown to be a prognostic marker in a number of human cancers, including esophageal-squamous cancers, non-small lung cancers, primary gastric cancers, malignant melanomas, prostate cancers, bladder cancers, and pancreatic carcinomas. The universality of S100A4 expression in a variety of cancers illustrates the potential use of S100A4 as a marker for tumor metastasis and disease progression (2).

General readings

Sherbet GV., et al. (2009) Cancer Lett. 280(1):15-30.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

S100A4 (C-term) antibody

  Goat Polyclonal Antibody against S100A4  
TA303160 (0.03µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

S100A4 antibody

  Mouse Monoclonal S100A4 Antibody (1F11)  
Western Blot: S100A4 Antibody (1F11) [TA337224] - Western blot analysis of S100A4 (1F11) in A375 and A549 cell lysate. Mouse IgG2b 1F11 Purified Hu C, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

S100A4 antibody

  Mouse Monoclonal S100A4 Antibody (1C4)  
Western Blot: S100A4 Antibody (1C4) [TA337225] - WB analysis of S100A4 in lysates of  A375 and A549 cell lines with S100A4 antibody (clone 1C4). The antibody detected the endogenous protein at its expected molecular weight position 11.7 kDa. Mouse IgG2b 1C4 Purified Hu C, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

S100A4 (C-term) antibody

  FSP1 / S100A4 Goat Polyclonal (C-Terminus) Antibody  
Anti-S100A4 antibody IHC of human uterus, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Goat IgG Aff - Purified Can, Hu, Ms, Rt E, P, WB
50 µg / €400.00
  OriGene Technologies, Inc.

S100A4 antibody

  Anti-S100A4 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human stomach cancer tissue . Recommended dilution: 1/100-200  The image on the left is immunohistochemistry of paraffin-embedded Human stomach cancer tissue using S100A4 antibody at dilution 1/100, on the right is treated with the fusion protein. (Original magnification: x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

S100A4 antibody

  Anti-S100A4 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue . Recommended dilution: 1/100-300  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using S100A4 antibody at dilution 1/80, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

S100A4 antibody

  Anti-S100A4 Rabbit Polyclonal Antibody  
Predicted band size: 12 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

S100A4 antibody

  Rabbit Polyclonal Anti-S100 A4 Antibody  
Western blot analysis of S100 A4 expression in Human Placenta lysate Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

S100A4 antibody

  Rabbit Polyclonal anti-S100A4 Antibody  
Western Blot analysis of placenta cell lysate with S100A4 antibody Rabbit Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

S100A4 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : A549 cell lysate 
Lane 3. : NIH/3T3 cell lysate 
Lane 4. : 3T3-L1 cell lysate Mouse IgG2b AT1C3 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

S100A4 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : A549 cell lysate 
Lane 3. : NIH/3T3 cell lysate 
Lane 4. : 3T3-L1 cell lysate Mouse IgG2b AT1C3 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

S100A4 (full length) antibody

Immunohistochemistry: AP08857PU-N S100A4 antibody staining of Formalin-Fixed Paraffin-Embedded Human Heart at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Aff - Purified Hu E, P
50 µl / €470.00
  Acris Antibodies GmbH

S100A4 (full length) antibody

Small intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu P
50 µg / €470.00
  Acris Antibodies GmbH

S100A4 antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Ig Fraction Bov, Can, Eq, Fe, Hu, Ms, Rt P
0.1 ml / €470.00
  Acris Antibodies GmbH

S100A4 antibody

FACS analysis of BOSC23 cells using NJ-4F3 Antibody Cat.-No DM122:. BOSC23 cells were transiently transfected with anexpression vector encoding either S100A4 (red curve) or anirrelevant protein (Control transfectant: black curve). Binding of NJ-4F3 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with  S100A4 trans-fected cells. Mouse IgG1 NJ-4F3 Purified Hu E, E(capture), E(detection), F
0.1 mg / €360.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

S100A4 - human gene knockout kit via CRISPR

S100A4 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

S100A4 - human gene knockout kit via CRISPR

S100A4 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

S100A4 - human gene knockout kit via CRISPR

S100A4 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

S100A4 - human gene knockout kit via CRISPR

S100A4 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

S100a4 - mouse gene knockout kit via CRISPR

S100a4 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

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