S100A8 / Calgranulin-A / MRP8 antibody

Principal name

S100A8 / Calgranulin-A / MRP8 antibody

Alternative names for S100A8 / Calgranulin-A / MRP8 antibody

S100-A8, CAGA, MRP-8, CFAG, S100 calcium-binding protein A8, Cystic fibrosis antigen, Migration inhibitory factor-related protein 8, Leukocyte L1 complex light chain, Calprotectin L1L subunit, Urinary stone protein band A

SwissProt ID

P05109 (Human), P27005 (Mouse), P28782 (Bovin), P50115 (Rat)

Gene ID

6279 (S100A8), 6280 (S100A9), 20201 (S100a8)

Available reactivities

Hu (Human), Por (Porcine), Rt (Rat), Can (Canine), Fe (Feline), GP (Guinea Pig), Mky (Monkey), Ms (Mouse), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Dot blot (Dot), Flow Cytometry (F), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of S100A8 / Calgranulin-A / MRP8 antibody

S100A8 is a low-molecular weight member of the S100 family of calcium-binding protein which promotes tumorigenesis. It promotes cell migration and invasion through p38 MAP dependent NF-kappaB activation leading to an increase of MMP2 and MMP12 in gastric cancer (kwon CH et al., 2013). The phagocyte-specific Ca2+-binding S100A8 protein has been proposed as an essential regulator of the plasma membrane DPH oxidase activity. It is abundantly expressed in the cytosol of neutrophils and is able to form Ca2+-dependent heterocomplexes, with heterotetramers being a probable prerequisite for its biological activities in myeloid cells. S100A8 and S100A9 have been proposed as essential regulators that exert their role through interactions with DPH oxidase subunits (Brechard S et al., 2013). S100A8 and S100A9 are generally considered proinflammatory. Whereas hypohalous acids generated by activated phagocytes promote novel modifications in murine S100A8 but modifications to human S100A8 are undefined and there is no evidence that these proteins scavenge oxidants in human disease. Oxidized S100A8 was prominent in lungs from patients with asthma and significantly elevated in sputum compared to controls. Results have broad implications for conditions under which hypohalous acid oxidants are generated by activated phagocytes. Identification in human disease of the novel S100A8 Cys derivatives typical of those generated in vitro strongly supports the notion that S100A8 contributes to antioxidant defense during oxidative stress (Gomes LH et al., 2013). PsA (Psoriatic arthritis) is a chronic inflammatory arthropathy associated with psoriasis. Histologically, PsA is characterized by lining layer hyperplasia, diffuse infiltrate of B, T, macrophages and dendritic cells associated with neutrophils' proliferation and angiogenesis. Histological findings are associated with monocyte-derived cytokines expression, as Myeloid-related protein (S100A8/A9). They play an important role in intracellular functions and cytoskeleton-membrane interactions. S100A8/A9 has a role in the propagation and perpetuation of the inflammatory process in patients with psoriasis and PsA, because of an activated monocyte/macrophage system that involve, distal to the skin, the "enthesal-complex." It also acts as one of the principal mediators of inte immune response, and has been proposed as diagnostic marker for several inflammatory conditions including neotal sepsis. (Chimenti MS et al. 2013, Terrin G et al., 2012).

General readings

ODINK, K., et al.: Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis. Nature 330, 80 (1987).
BRANDTZAEG, P., et al.: Mac387 antibody and detection of formalin resistant myelomonocytic L1 protein. J.Clin.Pathol.: 41, 963- (1988).
ZWADLO, G., et al.: Two calcium-binding proteins associated with specific stages of myeloid cell differen-tiation are expressed by subsets of macrophages in inflammatory tissues. Clin.Exp.Immunol.: 72, 510- (1988).
HOGG, N., et al.: Monoclonal antibody 5.5 reacts with p8,14, a myeloid molecule associated with some vascular endothelium. Eur. J. Immunol.: 19, 1053- (1989).
MURAO,S.,et al.: A Protein Containing the Cystic Fibrosis Antigen is an Inhibitor of Protein Kinases. J. Biol. Chem.: 254, (14) 8356 - 8360 (1989).
MUES, B., et al.: Phenotyping of macrophages with monoclonal antibodies in endomyocardial biopsies as a new approach to diagnosis of myocarditis. Eur. Heart Journal: 11, 619- (1990).
DELABIE, J., et al.: Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study. Clin.Exp.Immunol.: 81, 123- (1990).
TEIGELKAMP, S., et al.: Calcium dependent Complex Assembly of the Myeloic Differentiation Proteins MRP-8 and MRP-14. J. Biological Chem.: 266, (20), 13462 - 13467, (1991).
KUMAR, R.K., et al.: Dimeric S100A8 in human neutrophils is diminished after phagocytosis. J. Leukoc. Biol.: 70, 59-64 (2001).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

S100A8 / Calgranulin-A / MRP8 antibody

Immunohistochemistry on Human Tonsil Paraffin Sections using S100A8 antibody BM4028 clone 8-5C2. Mouse IgG1 8-5C2 Purified Hu C, E, P, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

S100A8 / Calgranulin-A / MRP8 antibody

BM4028B Calgranulin-A / MRP8 antibody staining of Human Tonsil Frozen Section. Mouse IgG1 8-5C2 Biotin Hu C, E, P, WB
0.1 mg / €500.00
  Acris Antibodies GmbH

S100A8 / Calgranulin-A / MRP8 antibody

Frozen Section of Human Tonsil stained with S100A8 Antibody Cat.-No BM4029 Clone S13.67 Mouse IgG1 S13.67 Purified Bov, Hu, Por, Rt C, E, F, P, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

S100A8 / Calgranulin-A / MRP8 antibody

(0.03ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

S100A8 / Calgranulin-A / MRP8 antibody

Predicted band size: 11 kDa . Positive control: K562 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

S100A8 / Calgranulin-A / MRP8 antibody

Western blot analysis of extracts of THP-1 cell lines, using S100A8 antibody. Rabbit IgG Purified Hu, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

S100A8 / Calgranulin-A / MRP8 antibody

Western Blot: S100A8/A9 Antibody (48M7C7) [TA337124] - analysis of S100A8 in A) recombinant protein, B) human spleen, C) HL60 and D) PC3 lysate using S1008A antibody at 0.5 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate soluti Mouse IgG2b 48M7C7 Purified Hu, Ms C, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

S100A8 / Calgranulin-A / MRP8 antibody

Western Blot: S100A8/A9 Antibody (48M5F4) [TA337126] - Analysis of S100A8 in recombinant protein using S1008A antibody at 0.5 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Mouse IgG1 48M5F4 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

S100A8 / Calgranulin-A / MRP8 antibody

Western Blot: S100A8 Antibody (63N13G5) [TA337132] - Analysis of S100A8 in 1) recombinant protein 2) mouse spleen and 3) mouse lung lysate using S1008A antibody at 1 ug/ml. goat anti-rat Ig HRP secondary antibod Rat IgG2b 63N13G5 Purified Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

S100A8 / Calgranulin-A / MRP8 antibody

S100A8 / Calgranulin-A / MRP8 Mouse IgM 7C12/4 Purified Hu E, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

S100A8 / Calgranulin-A / MRP8 antibody

S100A8 / Calgranulin-A / MRP8 Mouse IgG2b CF-145 Biotin Hu F, ICC/IF, P, WB
0.1 mg / €290.00
  Acris Antibodies GmbH

S100A8 / Calgranulin-A / MRP8 antibody

AM31838FC-N S100A8 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen at 1/50 followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2b CF-145 FITC Hu F, ICC/IF, P, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

S100A8 / Calgranulin-A / MRP8 antibody

AM31838PU-N S100A8 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Spleen, neutrophils at 2.5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG2b CF-145 Purified Hu F, ICC/IF, P, WB
0.2 mg / €340.00
  Acris Antibodies GmbH

S100A8 / Calgranulin-A / MRP8 antibody

S100A8 / Calgranulin-A / MRP8 Mouse IgG1 S13.67 Biotin Hu, Por, Rt C, E, F, P, WB
0.1 mg / €500.00
  Acris Antibodies GmbH

S100A8 / Calgranulin-A / MRP8 antibody

S100A8 / Calgranulin-A / MRP8 Mouse IgG1 S13.67 Purified Hu, Por, Rt C, E, F, P, WB
1 mg / €1,430.00
  Acris Antibodies GmbH

S100A8 / Calgranulin-A / MRP8 antibody

S100A8 / Calgranulin-A / MRP8 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

S100A8 / Calgranulin-A / MRP8 antibody

S100A8 / Calgranulin-A / MRP8 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

S100A8 / Calgranulin-A / MRP8 antibody

S100A8 MaxPab polyclonal antibody. Western Blot analysis of S100A8 expression in human lung cancer. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

S100A8 / Calgranulin-A / MRP8 antibody

S100A8 MaxPab polyclonal antibody. Western Blot analysis of S100A8 expression in human lung cancer. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

S100A8 / Calgranulin-A / MRP8 antibody

Western Blot analysis of S100A8 expression in transfected 293T cell line (H00006279-T04) by S100A8 MaxPab polyclonal antibody.

Lane 1: S100A8 transfected lysate(10.80 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

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