S100B antibody

Principal name

S100B antibody

Alternative names for S100B antibody

Protein S100-B, S100 beta, S100 calcium-binding protein B, S-100 protein subunit beta, S-100 protein beta chain, Astrocyte Marker

SwissProt ID

P02638 (Bovin), P04271 (Human), P04631 (Rat), P50114 (Mouse), Q6YNR6 (Rabit)

Gene ID

6285 (S100B), 525716, 20203 (S100b)

Available reactivities

Bov (Bovine), Fe (Feline), Hu (Human), Por (Porcine), Rb (Rabbit), Rt (Rat), Can (Canine), Gt (Goat), Sh (Sheep), Ms (Mouse)

Available hosts

Mouse, Rabbit, Goat, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), ELISA (capture) (E(capture)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R), Flow Cytometry (F)

Background of S100B antibody

S100 is a set of small, thermolabile, highly acidic dimer proteins, of approx. 20 kDa, widely distributed in different tissues. Dimeric combinations of two chains; the alpha chain (93 a.a. 10.4 kDa) and the beta chain (91 a.a. 10.5 kDa), form the three known subtypes of S100 [S100ao (alpha alpha), S100a (alpha beta) and S100b (beta beta). Although there is slight variation in the primary structure in different species, the S100 molecule is markedly conserved in the amino acid sequence, and the protein extracted from different organs of the same species is identical. The alpha and beta chains are 58% homologous (54 a.a.) and both have divalent cation binding sites situated toward the carboxy terminus and apparently similar functional features. S100 can be grouped with other calcium binding proteins, to which it has a significant sequence homology, particularly around the calcium binding domain, such as calmodulin, parvalbumin, intestinal calcium binding protein, myosin light chain, and troponin-C. Hence, S100 is a calcium modulated protein that binds calcium and zinc ions reversibly at physiologic pH and ionic strength, followed by a conformational change in the molecule. S100 is considered to be a cell-growth regulator, but other functions have been suggested, e.g., increasing the membrane permeability to cations under physiologic conditions, stimulation of nucleolar RNA polymerase activity, interaction with the tumor suppressor protein p53 and as a carrier of proteins and free fatty acids in adipocytes. Human S100 containing cells are subdivided to three groups, S100b containing cells (such as Schwann cells, pituicytes of the neurohypophysis, Langerhan

General readings

Gilquin B., et al. (2010) Mol Cell Biol. 30(11): 2724-36.Yamaguchi F., et al. (2012) J Biol Chem. 287(17): 13787-98.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

S100 (alpha/beta) (N-term) antibody

S100 (alpha/beta) Rabbit IgG Aff - Purified Hu, Rt C, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

S100 (alpha/beta) (C-term) antibody

S100 (alpha/beta) Rabbit IgG Aff - Purified Hu, Rt C, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

S100 (beta/beta) antibody

Western Blot AP10302PU-N S100B antibody at 1/500 dilution (SDS-PAGE 15% disconineous gel). Rabbit IgG Aff - Purified Hu, Rt C, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

S100B antibody

AM20529SU-N S100B antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 9A11B9 Ascites Hu E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

S100B antibody

Human Colon stained with ) anti-S100B Antibody Cat.-No AM21045PU (Clone SP127). Rabbit IgG SP127 Purified Bov, Hu, Ms, Rt F, P
0.5 ml / €510.00
  Acris Antibodies GmbH

S100B antibody

Human Colon stained with ) anti-S100B Antibody Cat.-No AM21045PU (Clone SP127). Rabbit IgG SP127 Purified Bov, Hu, Ms, Rt F, P
1 ml / €730.00
  Acris Antibodies GmbH

S100B antibody

Human Colon stained with ) anti-S100B Antibody Cat.-No AM21045PU (Clone SP127). Rabbit IgG SP127 Purified Bov, Hu, Ms, Rt F, P
0.1 ml / €290.00
  Acris Antibodies GmbH

S100B antibody

ELISA: S100B Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

S100B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100B (RC200277, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100B. Mouse IgG1 OTI6C5 Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

S100B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100B (RC200277, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100B. Mouse IgG1 OTI2H7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

S100B antibody

Immunohistochemical analysis of paraffin-embedded human brain, cerebellun using S100B antibody Cat.-No AM06189SU-N with DAB staining. Mouse IgG1 9A11B9 Ascites Hu P, WB
0.1 ml / €460.00
  Acris Antibodies GmbH

S100B antibody

Lane 1: Rat small intestine tissue lysate: 
Lane 2: Human placenta tissue lysate: Mouse IgG1 SA-12 Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

S100B (C-term) antibody

Polyclonal Anti- S-100β Antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

S100B (Center) antibody

Western blot analysis in NCI-H460 cell line lysates (35 ug/lane) using S100B Antibody (Center) Cat.-No AP53778PU-N. This demonstrates the S100B/S-100 antibody detected the S100B/S-100 protein (arrow). Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

S100B antibody

S100B Mouse IgG1 SH-B1 Ascites Bov, Fe, Hu, Por, Rb, Rt E, P, WB
1 ml / €480.00
  Acris Antibodies GmbH

S100B antibody

S100B Mouse IgG1 SH-B1 Ascites Bov, Fe, Hu, Por, Rb, Rt E, P, WB
0.5 ml / €330.00
  Acris Antibodies GmbH

S100B (S100A/B) antibody

S100B Rabbit Purified Bov, Hu C, P
1 ml / €380.00
  Acris Antibodies GmbH

S100B (S100A/B) antibody

S100B Rabbit Purified Bov, Hu C, P
0.5 ml / €260.00
  Acris Antibodies GmbH

S100B antibody

S100B Mouse IgG1 SH-B1 Bov, Hu, Rb, Rt P
0.5 ml / €390.00
  Abnova Taiwan Corp.

S100B antibody

S100B Rabbit Hu E, IP, R, WB
0.4 mg / €390.00
  Abnova Taiwan Corp.

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