S100B antibody

Principal name

S100B antibody

Alternative names for S100B antibody

Protein S100-B, S100 beta, S100 calcium-binding protein B, S-100 protein subunit beta, S-100 protein beta chain, Astrocyte Marker

SwissProt ID

P02638 (Bovin), P04271 (Human), P04631 (Rat), P50114 (Mouse), Q6YNR6 (Rabit)

Gene ID

6285 (S100B), 525716, 20203 (S100b), 25742

Available reactivities

Bov (Bovine), Fe (Feline), Hu (Human), Por (Porcine), Rb (Rabbit), Rt (Rat), Ms (Mouse)

Available hosts

Mouse, Rabbit, Goat, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), ELISA (capture) (E(capture)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R), Flow Cytometry (F)

Background of S100B antibody

Despite the abundance of calcium in the body, only a fraction of (1%) is present in the ionic form in biological fluids and rest is bound to various calcium biding proteins such as calmodulin, S100, calretinin, calbindin, parvalbumins etc. The ionic concentration of calcium is regulated by calcium sensing receptors. These receptors activate trans-cellular transport of calcium across the apical membranes facilitated by a group of low molecular weight calcium binding proteins. The active transport of calcium is achieved by Sodium calcium exchanges and by calcium transport proteins. S100 protein is a member of low MW calcium binding protein family, has two subunits (alfa and beta) and is expressed in S100ab or S100 bb confirmations. Human S100b subunit is a 94 amino acid protein expressed in brain. There are 9 genes (S100A1 through S100A9) that are mapped on chromosome 21q22.2-q22.3. S100 proteins improve contractility of the heart muscle by various mechanisms involving handling of sarcoplasmic reticulum calcium by myofbirilar calcium ion responsiveness. Because of the predominant location of S100 proteins in the glial cells, the S100 protein is leaked in to extracellular matrix and in to the cerebrospinal fluid (CSF) upon CNS injury. The S100 protein is a reliable marker for measuring the extent of CNS and neuronal damage (1, 2). Measurement of S100 protein is also a useful tool in diagnosis of acute stroke (3) and in various brain haemorrhages due to ruptured aneurysm (4).

General readings

Gilquin B., et al. (2010) Mol Cell Biol. 30(11): 2724-36.Yamaguchi F., et al. (2012) J Biol Chem. 287(17): 13787-98.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

S100B antibody

  Rabbit Monoclonal anti-S100 beta Antibody  
Human colon stained with anti-S100 Beta antibody. Rabbit SP127 Purified Hu F, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

S100B antibody

AM20529SU-N S100B antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 9A11B9 Ascites Hu E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

S100B antibody

  Rabbit Polyclonal S100B Antibody  
ELISA: S100B Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

S100B antibody

  S100B (S100 beta) mouse monoclonal antibody, clone OTI6C5 (formerly 6C5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100B (RC200277, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100B. Mouse IgG1 OTI6C5 Purified Hu WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

S100B antibody

  S100B mouse monoclonal antibody, clone OTI2H7 (formerly 2H7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100B (RC200277, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100B. Mouse IgG1 OTI2H7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

S100B antibody

Immunohistochemical analysis of paraffin-embedded human brain, cerebellun using S100B antibody Cat.-No AM06189SU-N with DAB staining. Mouse IgG1 9A11B9 Ascites Hu P, WB
0.1 ml / €460.00
  Acris Antibodies GmbH

S100B antibody

Lane 1: Rat small intestine tissue lysate: 
Lane 2: Human placenta tissue lysate: Mouse IgG1 SA-12 Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

S100B (C-term) antibody

Polyclonal Anti- S-100β Antibody Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

S100B (Center) antibody

Western blot analysis in NCI-H460 cell line lysates (35 ug/lane) using S100B Antibody (Center) Cat.-No AP53778PU-N. This demonstrates the S100B/S-100 antibody detected the S100B/S-100 protein (arrow). Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

S100B antibody

S100B Mouse IgG1 SH-B1 Ascites Bov, Fe, Hu, Por, Rb, Rt E, P, WB
1 ml / €500.00
  Acris Antibodies GmbH

S100B antibody

S100B Mouse IgG1 SH-B1 Ascites Bov, Fe, Hu, Por, Rb, Rt E, P, WB
0.5 ml / €330.00
  Acris Antibodies GmbH

S100B (S100A/B) antibody

S100B Rabbit Purified Bov, Hu C, P
1 ml / €390.00
  Acris Antibodies GmbH

S100B (S100A/B) antibody

S100B Rabbit Purified Bov, Hu C, P
0.5 ml / €260.00
  Acris Antibodies GmbH

S100B (bb+ab) antibody

S100B Mouse IgG2a 4B3 Purified Hu WB
1 mg / €420.00
  HyTest Ltd.

S100B (bb+ab) antibody

S100B Mouse IgG1 8B10 Purified Hu E, E(capture), WB
1 mg / €420.00
  HyTest Ltd.

S100B antibody

S100B staining of paraffin-embedded human Alzheimer’s brain tissue sections. Tissues were stained using HRP-DAB(brown) and counterstained with hematoxylin (blue). Goat Purified Hu C, E, ICC/IF, P, WB
0.1 mg / €450.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

S100b - mouse gene knockout kit via CRISPR

S100b - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

S100b - mouse gene knockout kit via CRISPR

S100b - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

S100b - mouse gene knockout kit via CRISPR

S100b - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

S100b - mouse gene knockout kit via CRISPR

S100b - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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