S100P antibody

Principal name

S100P antibody

Alternative names for S100P antibody

S100-P, S100-E, S100E, S100 calcium-binding protein P

SwissProt ID

P25815 (Human)

Gene ID

6286 (S100P)

Available reactivities

Hu (Human)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of S100P antibody

S100P was originally described as a placental protein of 95 amino acid residues shares about 50% sequence identity with the brain S100 proteins alpha and beta. S100 proteins are small dimeric members of the EF-hand superfamily of Ca(2+) binding proteins thought to participate in mediating intracellular Ca(2+) signals by binding to and thereby regulating target proteins in a Ca(2+)-dependent manner. S100P is dysregulated in the androgen-independent prostate cancer cell lines LNCaP-R, DU145, and PC3 and may play a role in the etiology of prostate cancer. In ductal hyperplasias, in situ and invasive ductal carcinoma, but not in the normal tissues, S100P overexpression is an early event that might play an important role in the imortalization of human breast epithelial cells in vitro and tumor progression in vivo. In NIH3T3 cells, the expression of S100P led to the presence of S100P in the culture medium, increased cellular proliferation, and enhanced survival following detachment from the culture substrate or after exposure to the chemotherapeutic agent 5-flurouracil. The proliferation and survival effects of S100P expression were duplicated in a time- and concentration-dependent manner by extracellular addition of purified S100P to wildtype NIH3T3 cells and correlated with the activation of Erks and NF- B. To determine the mechanisms involved in these effects, we tested the hypothesis that S100P activated RAGE (Receptor for Activated Glycation End-Products). It was found that S100P co-immunoprecipitated with RAGE. Furthermore, the effects of S100P on cell signaling, proliferation and survival were blocked by agents that interfere with RAGE including administration of an amphoterin derived peptide known to antagonize RAGE activation, anti-RAGE antibodies and by expression of a dominant negative RAGE. These data suggest that S100P can act in an autocrine manner via RAGE to stimulate cell proliferation and survival.

General readings

Dowen S.E., et al (2005) Am. J. Pathol. 166:81-92.
Koltzscher M, et al (2003) Mol Biol Cell. 14(6):2372-84.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

S100P antibody

S100P Mouse IgG1 UMAB24 Purified Hu WB
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

S100P antibody

S100P Mouse IgG1 UMAB24 Purified Hu WB
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

S100P antibody

Immunohistochemistry-Paraffin: S100P Antibody (EPR6142) [NBP1-95904] - Analysis of paraffin-embedded human pancreatic adenocarcinoma tissue using anti-S100P. Rabbit EPR6142 Purified Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

S100P antibody

Immunohistochemistry staining of paraffin Pancreatic ductal adenocarcinoma, urothelial carcinoma, placenta tissue by S100P antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic, nuclear) Mouse IgG1 16/f5 Purified Hu P
1 ml / €650.00
  OriGene Technologies, Inc.

S100P antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100P (RC201533, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100P. Mouse IgG1 OTI1F1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

S100P antibody

Anti-S100P mouse monoclonal antibody (TA504478) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY S100P(RC201533). Mouse IgG1 OTI1A4 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

S100P antibody

Detection limit for recombinant GST tagged S100P is approximately 3ng/ml as a capture antibody. Mouse IgG2b 4E7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

S100P antibody

S100P Mouse IgG2b 4E7 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

S100P antibody

S100P Mouse IgG2a 1A11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

S100P antibody

Western blot analysis of Human S100P polyclonal antibody ( Cat # PAB0011 ) in HeLa cell lysate. Rabbit Purified Hu E, WB
50 µg / €390.00
  Abnova Taiwan Corp.

S100P antibody

Immunohistochemistry-Paraffin: S100P Antibody [NBP1-89541] - Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

S100P

S100P Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

S100P

S100P Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €399.00
  OriGene Technologies, Inc.

S100P (1-95, His-tag)

S100P Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

S100P (1-95, His-tag)

S100P Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

S100P

12% SDS-PAGE separation of Human S100P 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 5µg/lane 3. non-reduced and non-heated sample, 5µg/lane Human Purified > 95 % > 95% E. coli
0.1 mg / €470.00
  Acris Antibodies GmbH

S100P

S100P Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

S100P

S100P Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

S100P

S100P Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

S100P

SDS-Page: S100P Protein [NBP1-44442] - S100P, 12.6kDa (115aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE Human Purified
0.1 mg / €340.00
  Novus Biologicals Inc.

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