SAE1 / AOS1 antibody

Principal name

SAE1 / AOS1 antibody

Alternative names for SAE1 / AOS1 antibody

SUA1, UBLE1A, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A

SwissProt ID

A2VE14 (Bovin), Q6AXQ0 (Rat), Q9R1T2 (Mouse), Q9UBE0 (Human)

Gene ID

10055 (SAE1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chimp (Chimpanzee), Chk (Chicken), Mky (Monkey)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), EPair for Elisa (EPair), ELISA (detection) (E(detection))

Background of SAE1 / AOS1 antibody

SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitinlike SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1; the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.

General readings

Desterro JM, Rodriguez MS, Kemp GD, Hay RT.
Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1.
J Biol Chem. 1999 Apr 9;274(15):10618-24.
PMID: 10187858

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SAE1 / AOS1 (C-term) antibody

The AOS1 C-term Pab (Cat. #TA302053) is used in Western blot to detect AOS1 in mouse heart tissue lysate. Rabbit Ig Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SAE1 / AOS1 antibody

Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308733 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SAE1 / AOS1 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506071) Mouse IgG2b OTI1G8 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SAE1 / AOS1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805142) Mouse IgG1 OTI6C7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SAE1 / AOS1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805143) Mouse IgG2b OTI6B11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SAE1 / AOS1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SAE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805319) Mouse IgG1 OTI3C3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SAE1 / AOS1 antibody

Immunoperoxidase of monoclonal antibody to SAE1 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3ug/ml] Mouse IgG1 1G4-1G5 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SAE1 / AOS1 (C-term) antibody

TA302596 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SAE1 / AOS1 antibody

Western blot analysis of extracts from 293 cells, using AOS1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SAE1 / AOS1 antibody

Western blot analysis of SAE1 in SK-N-SH lysate with SAE1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SAE1 / AOS1 antibody

Western blot analysis on 293 cell lysate using AOS1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SAE1 / AOS1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1 (RC201820, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE1. Mouse IgG2b OTI3C11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SAE1 / AOS1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAE1 (RC201820, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAE1. Mouse IgG1 OTI2B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SAE1 / AOS1 antibody

Formalin-Fixed, Paraffin-Embedded Human Prostate stained with SAE1 Antibody Cat.-No AM50084PU-N Mouse IgG2b 1G8 Purified Hu ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

SAE1 / AOS1 antibody

Western blot (WB) analysis of AOS1 antibody (Cat.-No.: AP06444PU-N) in extracts from 293 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SAE1 / AOS1 (full length) antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Spleen. Rabbit Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SAE1 / AOS1 antibody

Coomassie-stained SDS-PAGE of GST-SAE1 recombinant protein
(Panel A) and western blotting (Panel B) of HeLa WC lysate (lane 1) and purified recombinant GST-SAE1 (lane 2) are presented to show specificity of purified anti-SUMO Activating Enzyme (SAE1) antibody. The recombinant protein (with tag) ~60 kDa band present in 35 µg lysate (green, 800 nm channel) is indicated by the arrowhead. Lane 2 contains 50 ng of purified recombinant GST-SAE1 and lane 3 contains 300 ng of purified GST. Proteins were separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:2,000. Incubation was overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature. Molecular weight markers are shown for both the coomassie-stained gel and the western blot (lane M, red, 700 nm channel). IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Rabbit IgG Purified Bov, Can, Chimp, Hu, Ms, Rt E, ICC/IF, P, WB
0.5 mg / €440.00
  Acris Antibodies GmbH

SAE1 / AOS1 pSer185 antibody

Western blot using Rabbit-anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Rabbit IgG Aff - Purified Bov, Can, Chimp, Hu, Ms, Rt E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

SAE1 / AOS1 pSer185 antibody

Western blot using Rabbit-anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Rabbit IgG Aff - Purified Bov, Can, Chimp, Hu, Ms, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

SAE1 / AOS1 antibody

Immunofluorescence of purified MaxPab antibody to SAE1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

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