SAE2 / UBA2 antibody

Principal name

SAE2 / UBA2 antibody

Alternative names for SAE2 / UBA2 antibody

SUMO-activating enzyme subunit 2, UBLE1B, ARX, HRIHFB2115, Ubiquitin-like 1-activating enzyme E1B, Anthracycline-associated resistance ARX

SwissProt ID

P46946 (Yeast), Q7SXG4 (Danre), Q9UBT2 (Human), Q9Z1F9 (Mouse)

Gene ID

10054 (UBA2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP)

Background of SAE2 / UBA2 antibody

Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SAE2 / UBA2 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UBA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502504) Mouse IgG2a OTI4A3 Purified Can, Hu, Mky, Rt ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SAE2 / UBA2 (N-term) antibody

SAE2 / UBA2 Goat Aff - Purified Hu, Ms E
0.1 mg / €340.00
  Acris Antibodies GmbH

SAE2 / UBA2 antibody

Western blot analysis of extracts from 293 cells, treated with UV (5mins), using Uba2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SAE2 / UBA2 (C-term) antibody

Western blot analysis of SAE2 in 293 cell lysate with SAE2 antibody at 0.25 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SAE2 / UBA2 antibody

Western blot analysis on 293 cell lysate using Uba2 Antibody Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SAE2 / UBA2 antibody

Immunohistochemistry (IHC) analyzes of Uba2 antibody (Cat.-No.. AP06472PU-N) in paraffin-embedded human lung carcinoma tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SAE2 / UBA2 (160-210) antibody

Breast: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SAE2 / UBA2 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SAE2 / UBA2 (C-term) antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SAE2 / UBA2 control peptide

Western blot analysis of SAE2 in 293 cell lysate with SAE2 antibody at 0.25 μg/ml in (A) the absence and (B) the presence of blocking peptide.
50 µg / €160.00
  Acris Antibodies GmbH

SAE2 / UBA2 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the UBA2 polyclonal antibody ( Cat # PAB2233 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SAE2 / UBA2 antibody

SAE2 / UBA2 Goat Hu, Ms E
0.1 mg / €400.00
  Abnova Taiwan Corp.

SAE2 / UBA2 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Uba2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
50 µg / €205.00
  Assay Biotech

SAE2 / UBA2 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Uba2 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €310.00
  Assay Biotech

SAE2 / UBA2 antibody

Immunohistochemical analysis of UBA2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SAE2 / UBA2 antibody

Immunohistochemical analysis of UBA2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SAE2 / UBA2 antibody

Immunohistochemical analysis of UBA2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SAE2 / UBA2

SAE2 / UBA2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

SAE2 / UBA2

SAE2 / UBA2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SAE2 / UBA2

SAE2 / UBA2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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