SAMSN1 antibody

Principal name

SAMSN1 antibody

Alternative names for SAMSN1 antibody

SAMSN-1, SH3-SAM adaptor protein, HACS1, SAM domain-containing protein SAMSN-1, SAM domain SH3 domain and nuclear localization signals protein 1, Hematopoietic adaptor containing SH3 and SAM domains 1

SwissProt ID

P57725 (Mouse), Q9NSI8 (Human)

Gene ID

64092 (SAMSN1)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of SAMSN1 antibody

SAMSN1 is a member of a novel gene family of putative adaptors and scaffold proteins containing SH3 and SAM (sterile alpha motif) domains (Claudio et al., 2001 [PubMed 11536050]).[supplied by OMIM, Mar 2008]. Transcript Variant: This variant (2) contains alterte exons at the 5' end and initiates translation from an alterte start codon compared to variant 1. The resulting longer isoform (2) has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordites used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combition :: AF218085.2 [ECO:0000332] Rseq introns :: mixed/partial sample support ERS025081, ERS025082 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

General readings

Uchida T, Nakao A, Nakano N, et al. Identification of Nash1, a novel protein containing a nuclear localization signal, a sterile alpha motif, and an SH3 domain preferentially expressed in mast cells. Biochem. Biophys. Res. Commun. 2001; 288:137-41.
Claudio JO, Zhu YX, Benn SJ, et al. HACS1 encodes a novel SH3-SAM adaptor protein differentially expressed in normal and malignant hematopoietic cells. Oncogene 2001; 20:5373-7.
Zhu YX, Benn S, Li ZH, et al. The SH3-SAM adaptor HACS1 is up-regulated in B cell activation signaling cascades. J. Exp. Med. 2004; 200:737-47.
Wang D, Stewart AK, Zhuang L, et al. Enhanced adaptive immunity in mice lacking the immunoinhibitory adaptor Hacs1. FASEB J. 2010; 24:947-56.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SAMSN1 (particular isoform) antibody

TA302597 (1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SAMSN1 (C-term) antibody

Western blot analysis of SAMSN in Hela cell lysate with SAMSN antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SAMSN1 antibody

WB Suggested Anti-SAMSN1 Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SAMSN1 antibody

WB Suggested Anti-SAMSN1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SAMSN1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMSN1 (RC203721, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMSN1.(1:2000) Mouse IgG1 OTI2D8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SAMSN1 antibody

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SAMSN1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808900)(1:150) Mouse IgG1 OTI7F5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SAMSN1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMSN1 (RC203721, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMSN1.(1:2000) Mouse IgG1 OTI7C12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

SAMSN1 (C-term) Control Peptide

SAMSN1
50 µg / €160.00
  Acris Antibodies GmbH

SAMSN1 antibody

Western Blot analysis of SAMSN1 expression in transfected 293T cell line (H00064092-T01) by SAMSN1 MaxPab polyclonal antibody.

Lane 1: SAMSN1 transfected lysate(41.03 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SAMSN1 antibody

Western Blot analysis of SAMSN1 expression in transfected 293T cell line (H00064092-T01) by SAMSN1 MaxPab polyclonal antibody.

Lane 1: SAMSN1 transfected lysate(41.03 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

SAMSN1 antibody

SAMSN1 Mouse IgG1 2B-5A2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SAMSN1 antibody

SAMSN1 Mouse IgG1 1B8 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

SAMSN1 antibody

SAMSN1 Mouse IgG1 2B5-5A2 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

SAMSN1 antibody

SAMSN1 polyclonal antibody ( Cat # PAB6673 ) ( 1 µg/ml ) staining of Jurkat lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

SAMSN1 (Center) antibody

Human MCF-7; WB Suggested Anti-SAMSN1 Antibody Titration: 0.2-1 ug/ml. Positive Control: MCF7 cell lysate; SAMSN1 antibody - middle region (ARP45874_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

SAMSN1 antibody

Human Muscle; WB Suggested Anti-SAMSN1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Muscle; SAMSN1 antibody - middle region (ARP45875_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SAMSN1 antibody

Western Blot: SAMSN1 Antibody [NBP1-52845] - This Anti-SAMSN1 antibody was used in Western Blot of Fetal Muscle tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

SAMSN1 antibody

Immunohistochemistry-Paraffin: SAMSN1 Antibody [NBP1-82598] - Immunohistochemical staining of human testis shows strong positivity in Leydig cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

SAMSN1 antibody

Immunohistochemistry-Paraffin: SAMSN1 Antibody [NBP1-82599] - Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SAMSN1 (full length, N-term HIS tag)

SAMSN1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

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