Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of Sarcolipin (SLN) antibody
Sarcolipin is a 31 amino acid integral membrane protein belonging to the sarcolipin family. The gene encoding for the protein maps to chromosome 11q22-q23. SLN, localizing to the sarcoplasmic reticulum, regulates Ca-ATPase activity in skeletal muscle. It associates with calcium ATPase SERCA1 (Sarco(endo)plasmic reticulum Ca2+-ATPases). In muscle tissue, SERCAs are regulated by sarcolipin and another membrane protein, phospholamban (PLN). SLN is expressed in high levels in fast-twitch muscle, which has high expression of SERCA1a, and in lower levels in cardiac muscle. In rats, however, expression of SLN is higher in cardiac muscle than in fast-twitch muscle.
Western Blot: SLN Lysate [NBL1-16214] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for SLN
Western Blotting Analysis: Representative lot data. Rabbit quadriceps tissue lysate was probed with Anti-Sarcolipin (1.0 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and visualized using a chemiluminescence detection system. Arrow indicates Sarcolipin (~6 kDa). An uncharacterized band may be observed at ~13 kDa in some tissue lysates.