SARS ORF1ab polyprotein antibody

Principal name

SARS ORF1ab polyprotein antibody

Alternative names for SARS ORF1ab polyprotein antibody

Replicase polyprotein 1ab, rep 1a-1b

SwissProt ID

P0C6X7 (Cvhsa)

Gene ID

1489680

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunoelectron Microscopy (EM)

Background of SARS ORF1ab polyprotein antibody

Generally, viruses have proteases to process their proteins into active form. Because of its pivotal role in the viral life cycle, proteases are primary targets for the development of antiviral agents. 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CL protease is the only Coronavirus protein for which structural information is available. 3CL protease comprises three domains, the substrate-binding site is expected to be located between domains I and II, and domain III is a globular cluster comprising five helices. 3CL protease is a homodimer.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SARS ORF1ab polyprotein (NSP3) antibody

Immunofluorescence Microscopy using anti-SARS-CoV nsp3 antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX was used. Rabbit IgG Serum EM, ICC/IF, IP, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

SARS ORF1ab polyprotein (NSP8) antibody

Immunoprecipitation followed by western
blotting using Anti-nsp8 shows a predominant band at 21.8 kDa corresponding to full length SARS protein (panel A). Immunofluorescence Microscopy using anti-nsp8 6-h post infection of Vero-E6 cells (Panel B). For detection Cy3 conjugated Goat-anti-Rabbit IgG MX was used. Rabbit IgG Serum EM, ICC/IF, IP, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

SARS ORF1ab polyprotein (NSP13) antibody

Immunofluorescence Microscopy using anti-SARS-CoV nsp13 antibody, 6-h post infection Vero-E6 cells. For detection Cy3 conjugated Goat-anti-Rabbit IgG MX was used. Rabbit IgG Serum EM, ICC/IF, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

SARS ORF1ab polyprotein (3CL Protease) antibody

Western blot using Protein A Purified anti-SARS CoV 3CL Protease antibody shows detection of a 34-kDa band corresponding to the protein. Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose. The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h followed by detection using IRDye(TM)800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:10,000. The fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other  detection systems will yield similar results. Rabbit IgG Purified E, WB
0.5 mg / €480.00
  Acris Antibodies GmbH

SARS ORF1ab polyprotein antibody

Western blot using orf1ab polyclonal antibody ( Cat # PAB11368 ) shows detection of a 34-kDa band corresponding to the protein.
Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose.
The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h. Rabbit Hu, Ms E, ICC/IF, WB
0.5 mg / €560.00
  Abnova Taiwan Corp.

SARS ORF1ab polyprotein antibody

Immunofluorescence Microscopy using orf1ab polyclonal antibody ( Cat # PAB11369 ), 6 hrs post infection Vero-E6 cells.
Personal Communication, Eric Snijder, LeidenUniversity Medical Center, Leiden, Netherlands. Rabbit Hu, Ms E, ICC/IF, IP
0.1 ml / €560.00
  Abnova Taiwan Corp.

SARS ORF1ab polyprotein antibody

Immunofluorescence Microscopy using orf1ab polyclonal antibody ( Cat # PAB11370 ), 6 hrs post infection Vero-E6 cells.
Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands. Rabbit Hu, Ms E, ICC/IF, IP
0.1 ml / €560.00
  Abnova Taiwan Corp.

SARS ORF1ab polyprotein antibody

Immunofluorescence Microscopy using orf1ab polyclonal antibody ( Cat # PAB11371 ), 6 hrs post infection Vero-E6 cells.
Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands. Rabbit Hu, Ms E, ICC/IF, IP
0.1 ml / €560.00
  Abnova Taiwan Corp.

SARS ORF1ab polyprotein antibody

Immunofluorescence Microscopy using orf1ab polyclonal antibody ( Cat # PAB11372 ) 6 hrs post infection of Vero-E6 cells.
Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands. Rabbit Hu, Ms E, ICC/IF, IP, WB
0.1 ml / €560.00
  Abnova Taiwan Corp.

9 Item(s)

per page
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