SART1 antibody

Principal name

SART1 antibody

Alternative names for SART1 antibody

SART-1, SNU66 homolog, Homs-1, Hom s 1, U4/U6.U5 tri-snRNP-associated protein 1, U4/U6.U5 tri-snRNP-associated 110 kDa protein, Squamous cell carcinoma antigen recognized by T cells 1

SwissProt ID

O43290 (Human), Q5XIW8 (Rat), Q9Z315 (Mouse)

Gene ID

9092 (SART1)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Xen (Xenopus), Ze (Zebrafish)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of SART1 antibody

SART1 (HAF, Ara1, HOMS1, Snu66) is part of the SNU66/SART1 family. This protein is subject to phosphorylation, and sumoylation by SUMO2. It is highly expressed in the nucleus of highly proliferative cells, but is also expressed in the cytosol of epithelial cancers. HAF is a transcription factor that enhances hypoxia inducible factor 1 (HIF-1)-induced activation in VEGF and EPO genes under hypoxic conditions (http://www.aacrmeetingabstracts.org/cgi/content/abstract/2006/1/409-d). SART1/HAF is up-regulated in a multitude of tumors. During long periods of hypoxia, its level increases, but the levels are decreased during acute hypoxia. A recent study showed that SART1/HAF is an E3 ubiquitin ligase that binds and ubiquitites HIF-1 alpha by an oxygen and pVHL-independent mechanism, which then targets HIF-1 alpha for proteasomal degradation. SART1/HAF also binds HIF-2 alpha at a separate site and increases HIF-2 alpha transactivation without triggering degradation. Therefore, HAF is able to alter the response of cancer cells from HIF-1 alpha-dependent to HIF-2 alpha-dependent transcription and initiates genes involved in invasion such as MMP9, PAI-1, and the stem cell factor OCT-3/4. The shift to HIF-2 alpha dependent gene expression instigated by HAF also promotes an enhanced tumor stem cell population, which results in extremely aggressive tumors in vivo. SART1/HAF presents a method for more aggressive tumor growth under sustained hypoxia (PMID: 21512133; 18838541; 19377289)

General readings

Vertegaal AC, Ogg SC, Jaffray E, Rodriguez MS, Hay RT, Andersen JS, Mann M, Lamond AI.
A proteomic study of SUMO-2 target proteins.
J Biol Chem. 2004 Aug 6;279(32):33791-8. Epub 2004 Jun 2.
PMID: 15175327

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SART1 antibody

Sample (30 ug of whole cell lysate). A: Jurkat. B: Raji. C: K562. D: NCI-H929. 7.5% SDS PAGE. TA307946 diluted at 1:5000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SART1 (N-term) antibody

TA302845 (0.03µg/ml) staining of Hela lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SART1 antibody

Sample (30 ug of whole cell lysate). A: H1299. B: Hela. C: Molt-4 . 7.5% SDS PAGE. TA308280 diluted at 1:5000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SART1 antibody

Western blot analysis of extracts from JurKat cells, using SART1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SART1 antibody

Western Blot: SART1 Antibody [TA336906] - SART1 antibody tested in NIH-3T3 cell lysate. Rabbit Purified Hu, Ms C, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SART1 antibody

Immunofluorescence of purified MaxPab antibody to SART1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

SART1 antibody

SART1 polyclonal antibody ( Cat # PAB6298 ) staining ( 0.03 µg/ml ) of HeLa lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

SART1 antibody

NB 100-1145 (0.03µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

SART1 antibody

Western Blot and Immunoprecipitation: SART1 Antibody [NB100-68233] - Detection of Human SART1 on HeLa whole cell lysate using NB100-68233. SART1 was immunoprecipitated by rabbit anti-SART1 antibody NB100-68232. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SART1 antibody

Immunohistochemistry-Paraffin: SART1 Antibody [NBP1-89022] - Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

SART1 antibody

Immunohistochemistry-Paraffin: SART1 Antibody [NBP1-89023] - Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

SART1 antibody

Immunohistochemistry-Paraffin: SART1 Antibody [NBP1-89024] - Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SART1

SART1 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

SART1

SART1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

SART1

SART1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

SART1 293T Cell Transient Overexpression Lysate(Denatured)

SART1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

16 Item(s)

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