SCYE1 / EMAP2 antibody

Principal name

SCYE1 / EMAP2 antibody

Alternative names for SCYE1 / EMAP2 antibody

EMAP-2, EMAP-II, AIMP1, Small inducible cytokine subfamily E member 1, Endothelial monocyte-activating polypeptide 2, Endothelial monocyte-activating polypeptide II, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43

SwissProt ID

P31230 (Mouse), Q08601 (Yeast), Q12904 (Human)

Gene ID

9255 (AIMP1)

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rb (Rabbit), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Ye (Yeast)

Available hosts

Mouse, Goat, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of SCYE1 / EMAP2 antibody

SCYE1 is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tR synthetase complex. Therefore, pro-SCYE1 may function in binding R as part of the tR synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells. The protein encoded by this gene is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tR synthetase complex. Therefore, pro-SCYE1 may function in binding R as part of the tR synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

General readings

Zhu X., et al. (2009) Proc Natl Acad Sci U S A. 106(37):15944-9.
Kim E., et al. (2008) J Immunol. 180(5):2894-902.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SCYE1 / EMAP2 (137-149) antibody

AIMP1 antibody Cat.-No AP31988PU-N staining of paraffin embedded Human Pancreas at 5 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Hu, Por, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

  Rabbit polyclonal antibody to EMAP II (small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating))  
Sample(30 ug of whole cell lysate). A:H1299. B:Hep G2. 12% SDS PAGE. TA308323 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 (N-term) antibody

  Goat Anti-AIMP1 / SCYE1 Antibody  
(0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 antibody

  Rabbit Polyclonal AIMP1 Antibody  
Western blot analysis of AIMP1 in rat brain tissue lysate with AIMP1 antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 antibody

  Rabbit anti-AIMP1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using AIMP1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 antibody

  Rabbit Polyclonal Anti-SCYE1 Antibody  
WB Suggested Anti-SCYE1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate; AIMP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 antibody

  Rabbit Polyclonal Anti-SCYE1 Antibody  
WB Suggested Anti-SCYE1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysate; AIMP1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 antibody

  Rabbit Polyclonal Anti-AIMP1 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Hela cells, hepG2 cells, Primary antibody: (AIMP1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 second Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 (C-term) antibody

(LEFT) Western blot analysis of MCA1 antibody (C-term) (AP17551PU-N) in 293 cell line lysates (35ug/lane). MCA1 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of MCA1(arrow) using rabbit polyclonal MCA1 Antibody (N-term) (AP17551PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MCA1 gene (Lane 2) (Origene Technologies). Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

BM4115 EMAP2 antibody staining of Rat Spleen Paraffin Section. Mouse IgG 546-2 Purified Hu, Rt C, E, P, WB
0.2 mg / €530.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Mouse IgG2b 546-2 Purified Hu, Rt E, F, P, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Goat Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Goat Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Goat Aff - Purified Hu E, WB
50 µg / €230.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

AIMP1 - human gene knockout kit via CRISPR

AIMP1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

AIMP1 - human gene knockout kit via CRISPR

AIMP1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

AIMP1 - human gene knockout kit via CRISPR

AIMP1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

AIMP1 - human gene knockout kit via CRISPR

AIMP1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SCYE1 / EMAP2 (full length, N-term HIS tag, transcript variant 1)

  Purified recombinant protein of Human aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug  
SCYE1 / EMAP2 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

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