SCYE1 / EMAP2 antibody

Principal name

SCYE1 / EMAP2 antibody

Alternative names for SCYE1 / EMAP2 antibody

EMAP-2, EMAP-II, AIMP1, Small inducible cytokine subfamily E member 1, Endothelial monocyte-activating polypeptide 2, Endothelial monocyte-activating polypeptide II, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43

SwissProt ID

P31230 (Mouse), Q08601 (Yeast), Q12904 (Human)

Gene ID

9255 (AIMP1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Ye (Yeast)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Frozen Sections (C), Flow Cytometry (F)

Background of SCYE1 / EMAP2 antibody

SCYE1 is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tR synthetase complex. Therefore, pro-SCYE1 may function in binding R as part of the tR synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells. The protein encoded by this gene is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tR synthetase complex. Therefore, pro-SCYE1 may function in binding R as part of the tR synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

General readings

Zhu X., et al. (2009) Proc Natl Acad Sci U S A. 106(37):15944-9.
Kim E., et al. (2008) J Immunol. 180(5):2894-902.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SCYE1 / EMAP2 (137-149) antibody

AIMP1 antibody Cat.-No AP31988PU-N staining of paraffin embedded Human Pancreas at 5 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Hu, Por, Rt E, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

Sample(30 ug of whole cell lysate). A:H1299. B:Hep G2. 12% SDS PAGE. TA308323 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 (N-term) antibody

(0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 antibody

Western blot analysis of AIMP1 in rat brain tissue lysate with AIMP1 antibody at (A) 0.5 and (B) 1 ug/ml. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 antibody

Western blot analysis of extracts of various cell lines, using AIMP1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 antibody

WB Suggested Anti-SCYE1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate; AIMP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 antibody

WB Suggested Anti-SCYE1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysate; AIMP1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

SCYE1 / EMAP2 (C-term) antibody

(LEFT) Western blot analysis of MCA1 antibody (C-term) (AP17551PU-N) in 293 cell line lysates (35ug/lane). MCA1 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of MCA1(arrow) using rabbit polyclonal MCA1 Antibody (N-term) (AP17551PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MCA1 gene (Lane 2) (Origene Technologies). Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

BM4115 EMAP2 antibody staining of Rat Spleen Paraffin Section. Mouse IgG 546-2 Purified Hu, Rt C, E, P, WB
0.2 mg / €530.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Mouse IgG2b 546-2 Purified Hu, Rt E, F, P, WB
0.1 mg / €390.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Goat Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Goat Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Goat Aff - Purified Hu E, WB
50 µg / €230.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SCYE1 / EMAP2 antibody

Detection limit for recombinant GST tagged SCYE1 is approximately 1ng/ml as a capture antibody. Mouse IgG1 M1 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

SCYE1 / EMAP2 antibody

SCYE1 monoclonal antibody (M05), clone 3H5. Western Blot analysis of SCYE1 expression in Jurkat ( Cat # L017V1 ). Mouse IgG2a 3H5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SCYE1 / EMAP2 antibody

SCYE1 / EMAP2 Mouse IgG2a 1C11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SCYE1 / EMAP2 antibody

Human Jurkat; WB Suggested Anti-SCYE1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate. AIMP1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; SCYE1 antibody - N-terminal region (ARP40551_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

SCYE1 / EMAP2 antibody

Human HEK293T; WB Suggested Anti-SCYE1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate. AIMP1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells.; SCYE1 antibody - middle region (ARP40552_P050) in Human HEK293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

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