SCYL3 / PACE1 antibody
Principal name
SCYL3 / PACE1 antibody
Alternative names for SCYL3 / PACE1 antibody
SCY1-like protein 3, Ezrin-binding protein PACE-1, Protein-associating with the carboxyl-terminal domain of ezrin
Ncbi ID
9606, NP_851607, NM_028776, NP_065156.5, NM_020423, NP_065156, NP_083052.1, NM_181093
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), EPair for Elisa (EPair), Immunoprecipitation (IP), Paraffin Sections (P)
Background of SCYL3 / PACE1 antibody
- Kit Component:
- - KN315416G1, Scyl3 gRNA vector 1 in pCas-Guide vector
- KN315416G2, Scyl3 gRNA vector 2 in pCas-Guide vector
- KN315416D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunofluorescence of purified MaxPab antibody to SCYL3 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of SCYL3 expression in transfected 293T cell line (H00057147-T01) by SCYL3 MaxPab polyclonal antibody.Lane 1: SCYL3 transfected lysate(75.68 KDa).Lane 2: Non-transfected lysate.
SCYL3 MaxPab polyclonal antibody. Western Blot analysis of SCYL3 expression in HepG2.
Western Blot analysis of SCYL3 expression in transfected 293T cell line by SCYL3 monoclonal antibody (M01), clone 3D3.Lane 1: SCYL3 transfected lysate(76.7 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of SCYL3 expression in transfected 293T cell line (H00057147-T01) by SCYL3 MaxPab polyclonal antibody.Lane 1: SCYL3 transfected lysate(76.70 KDa).Lane 2: Non-transfected lysate.
Human COLO205; WB Suggested Anti-SCYL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: COLO205 cell lysate. SCYL3 is strongly supported by BioGPS gene expression data to be expressed in Human COLO205 cells.; SCYL3 antibody - N-terminal region (ARP57774_P050) in Human COLO205 cells using Western Blot
Mouse Liver; WB Suggested Anti-Scyl3 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Liver; Scyl3 antibody - middle region (ARP57775_P050) in Mouse Liver cells using Western Blot
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-SCYL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500487, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCYL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500487, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SCYL3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500487, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-SCYL3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500487, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SCYL3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500487, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-SCYL3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500487, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-SCYL3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500487, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (RC204859, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (RC204859, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SCYL3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500492, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SCYL3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500492, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (RC204859, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-SCYL3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500493, Dilution 1:50)
Anti-SCYL3 mouse monoclonal antibody (TA500493) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SCYL3(RC204859).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SCYL3 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL3 (RC204859, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCYL3.
Sample (30 ug of whole cell lysate). A: A431. 7.5% SDS PAGE. TA308183 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using SCY1 like 3(TA308183) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SCY1 like 3(TA308183) antibody at 1:200 dilution.
WB Suggested Anti-Scyl3 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Liver
WB Suggested Anti-SCYL3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: COLO205 cell lysateSCYL3 is strongly supported by BioGPS gene expression data to be expressed in Human COLO205 cells
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA500487 | SCYL3 / PACE1 antibody |
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| anti-SCYL3 mouse monoclonal antibody, clone OTI1A5 (formerly 1A5) | |||||||
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Mouse | IgG1 | OTI1A5 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
 +7 additional images |
Highly validated antibody!
|
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| TA500493 | SCYL3 / PACE1 antibody |
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| anti-SCYL3 mouse monoclonal antibody, clone OTI4F10 (formerly 4F10) | |||||||
 |
Mouse | IgG1 | OTI4F10 | Purified | Hu, Mky | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA308183 | SCYL3 / PACE1 antibody |
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| Rabbit polyclonal antibody to SCY1 like 3 (SCY1-like 3 (S. cerevisiae)) | |||||||
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Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€416.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA344608 | SCYL3 / PACE1 antibody |
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| Rabbit Polyclonal Anti-SCYL3 Antibody - middle region | |||||||
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Rabbit | IgG | Purified | Ms, Rb, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA344609 | SCYL3 / PACE1 antibody |
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| Rabbit Polyclonal Anti-SCYL3 Antibody - N-terminal region | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, Ms, Por, Rb, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA500489 | SCYL3 / PACE1 antibody |
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| anti-SCYL3 mouse monoclonal antibody, clone OTI7E7 (formerly 7E7) | |||||||
 |
Mouse | IgG2a | OTI7E7 | Purified | Hu | WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA500492 | SCYL3 / PACE1 antibody |
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| anti-SCYL3 mouse monoclonal antibody, clone OTI2H10 (formerly 2H10) | |||||||
 |
Mouse | IgG1 | OTI2H10 | Purified | Hu | P, WB |
0.1 ml /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN204859 | SCYL3 - human gene knockout kit via CRISPR |
||
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Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204859BN | SCYL3 - human gene knockout kit via CRISPR |
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|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204859LP | SCYL3 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN204859RB | SCYL3 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN315416 | Scyl3 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN315416BN | Scyl3 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN315416LP | Scyl3 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN315416RB | Scyl3 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304859 | SCYL3 / PACE1 (transcript variant 1) |
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| Recombinant protein of human SCY1-like 3 (S. cerevisiae) (SCYL3), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€438.00
|
|
| OriGene Technologies, Inc. | |||||
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Lysates
| Catalog No. | ||
|---|---|---|
| LY412474 | SCYL3 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC204859L2V | Lenti ORF particles, SCYL3 (mGFP-tagged) - Human SCY1-like 3 (S. cerevisiae) (SCYL3), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,082.00
|
|
| OriGene Technologies, Inc. | ||
| RC220028L2V | Lenti ORF particles, SCYL3 (mGFP-tagged)-Human SCY1-like 3 (S. cerevisiae) (SCYL3), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,679.00
|
|
| OriGene Technologies, Inc. | ||
| RC204859L1V | Lenti ORF particles, SCYL3 (Myc-DDK tagged) - Human SCY1-like 3 (S. cerevisiae) (SCYL3), transcript variant 1 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€969.00
|
|
| OriGene Technologies, Inc. | ||
