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Securin / PTTG1 antibody

Principal name

Securin / PTTG1 antibody

Alternative names for Securin / PTTG1 antibody

PTTG, EAP1, TUTR1, Pituitary tumor-transforming gene 1 protein, Esp1-associated protein, Tumor-transforming protein 1

SwissProt ID

O95997 (Human), P97613 (Rat), Q3SZY3 (Bovin), Q9BDP6 (Pig), Q9CQJ7 (Mouse)

Gene ID

9232 (PTTG1), 30939 (Pttg1), 64193

Ncbi ID

9606, NP_004210, NP_004210.1, NP_001124526.1, NM_022391, NM_004219

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rt (Rat), Mky (Monkey), Prim (Primate)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of Securin / PTTG1 antibody

PTTG1 is a homolog of yeast securing proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. PTTG1 has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. PTTG1 contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.

General readings

1. Herman-Antosiewicz, A., et al., Mol. Cancer Ther. 6, 1249-1261 (2007)
2. Toyoda, Y., et al., Mol. Biol. Cell 17, 2287-2302 (2006)
3. Gururaja, T. L., et al., Clin. Cancer Res. 12, 3831-3842 (2006)
4. Wang, H., et al., Genes. Dev. 15, 1361-1372 (2001)
5. Zou, H., et al., Science 285, 418-422 (1999)
Clone DCS-280 is used in the reference 1-3.

ARP38424_T100

Immunohistochemistry (IHC) analyzes of PTTG1/2/3 antibody (Cat.-No.: AP06655PU-N) in paraffin-embedded human testis tissue.

AP06655PU-N

Western blot (WB) analysis of PTTG1/2/3 antibody (Cat.-No.: AP06655PU-N) in extracts from HepG2 cells.

AP06655PU-N

Detection limit for recombinant GST tagged PTTG1 is approximately 0.03ng/ml as a capture antibody.

H00009232-M35

Human rectal carcinoma stained with anti-Pds1 antibody

Recombinant human PTTG1, 1-202 aa, His-tagged: 15% SDS-PAGE (3 µg)

Figure 1. AM00536AF-N Securin antibody staining of a squamous epitheliumof Human tonsil Paraffin Embedded Section.

AM00356AF-N

Figure 2. Western blot analysis of Securin expression in Raji (Lane 1), Jurkat (Lane 2), U251 (Lane 3), WR19L (Lane 4), C2C12 (Lane 5), A431 (Lane 6) and Rat-1 (Lane 7) using Securin antibody AM00356AF-N.

AM00356AF-N

Figure 3. Immunoprecipitation of Securin from Raji cells with Mouse IgG2a (Lane 2) or AM00356AF-N (Lane 3). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with Securin antibody (AM00356AF-N). Raji crude lysate was resolved in Lane 1.

AM00356AF-N

Immunofluorescence analysis of HuvEc cells, using PTTG1 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HepG2/Jurkat/293 cells, using PTTG1 Antibody. The lane on the right is treated with the synthesized peptide.

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23984PU-N

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23984PU-N

Immunohistochemical analysis of PTTG1/2/3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of PTTG1/2/3 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of PTTG1/2/3 expression in HepG2 (A); Jurkat (B); NIH3T3 (C) whole cell lysates.

TP311038

Western blot analysis of extracts from 293 cells, Jurkat cells and HepG2 cells, using PTTG1 antibody.The lane on the right is treated with the synthesized peptide.

TA312966

Immunofluorescence analysis of HUVEC cells, using PTTG1 antibody.The picture on the right is treated with the synthesized peptide.

TA312966

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human breast and lung cancer tissue . Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using PTTG1 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x 200)

TA322776

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human breast and lung cancer tissue . Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using PTTG1 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x200)

TA322776

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and gastric cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using PTTG1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200)

TA322777

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human colon and gastric cancer tissue . Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using PTTG1 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x200)

TA322777

Western blot analysis on Jurkat and K562 cell lysate using PTTG1 Antibody

TA347554

Human Rectal Carcinoma stained with anti-PDS-1_Securin antibody.

TA354022

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AP06655PU-N	PTTG1/2/3 antibody
	Rabbit			Aff - Purified	Hu	ICC/IF, P, WB	0.1 mg / €350.00
							Acris Antibodies GmbH
+1 additional image
TA312966	Securin / PTTG1 antibody
	Rabbit polyclonal anti-PTTG1 antibody
	Rabbit	IgG		Purified	Hu	E, ICC/IF, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA322776	Securin / PTTG1 antibody
	Anti-PTTG1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA322777	Securin / PTTG1 antibody
	Anti-PTTG1 Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA347554	Securin / PTTG1 antibody
	Rabbit Polyclonal Anti-PTTG1 Antibody
	Rabbit	IgG		Purified	Hu	WB	0.1 mg / €335.00
							OriGene Technologies, Inc.
AM00356AF-N	Securin / PTTG1 antibody
	Mouse	IgG2a	DCS-280	Azide Free	Hu, Ms, Rt	ICC/IF, IP, P, WB	0.1 mg / €360.00
							Acris Antibodies GmbH
+2 additional images
AP23984PU-N	Securin / PTTG1 (148-160) antibody
	Goat	IgG		Aff - Purified	Elephant, Hu, Mky, Ms	P	50 µg / €380.00
							Acris Antibodies GmbH
+1 additional image
AP55262SU-N	Securin / PTTG1 antibody
	Rabbit			Serum	Hu	C, E, WB	0.2 ml / €400.00
							Acris Antibodies GmbH
C10252-1	Securin / PTTG1 antibody
	Rabbit			Aff - Purified	Hu	E, ICC/IF, WB	50 µg / €230.00
							Assay Biotech
+1 additional image
C10252-2	Securin / PTTG1 antibody
	Rabbit			Aff - Purified	Hu	E, ICC/IF, WB	0.1 mg / €330.00
							Assay Biotech
+1 additional image

CRISPR

Catalog No.	Species	Donor Vector
KN314249	Pttg1 - mouse gene knockout kit via CRISPR
	Mouse	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN314249BN	Pttg1 - mouse gene knockout kit via CRISPR
	Mouse	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN314249LP	Pttg1 - mouse gene knockout kit via CRISPR
	Mouse	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN314249RB	Pttg1 - mouse gene knockout kit via CRISPR
	Mouse	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
TP311038	Securin / PTTG1
	Recombinant protein of human pituitary tumor-transforming 1 (PTTG1)
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €680.00
					OriGene Technologies, Inc.
AR09247PU-L	Securin / PTTG1 (1-202, His-tag)
	Human	Purified	> 80 % by SDS - PAGE	E. coli	0.5 mg / €1,000.00
					Acris Antibodies GmbH
AR09247PU-N	Securin / PTTG1 (1-202, His-tag)
	Human	Purified	> 80 % by SDS - PAGE	E. coli	0.1 mg / €370.00
					Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.
RC211038L2V	Lenti ORF particles, PTTG1 (mGFP-tagged) - Human pituitary tumor-transforming 1 (PTTG1), 200 uL, >10^7 TU/mL
		200 µl / €907.00
		OriGene Technologies, Inc.

RC211038L1V	Lenti ORF particles, PTTG1 (Myc-DDK tagged) - Human pituitary tumor-transforming 1 (PTTG1) , 200 uL, >10^7 TU/mL
		200 µl / €804.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR214617L2V	Lenti ORF particles, Pttg1 (GFP-tagged ORF) - Rat pituitary tumor-transforming 1 (Pttg1), 200 uL, >10^7 TU/mL
		200 µl / €907.00
		OriGene Technologies, Inc.

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