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SEMA4A antibody

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Principal name

SEMA4A antibody

Alternative names for SEMA4A antibody

Semaphorin-4A, Semaphorin-B, Sema B, SEMAB, SEMB

SwissProt ID

Q5EA85 (Bovin), Q62178 (Mouse), Q9H3S1 (Human)

Gene ID

64218 (SEMA4A)

Ncbi ID

9606, NP_071762, NP_001180229.1, NP_001180229, 10090, NM_022367, NM_001193302

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Flow Cytometry (F)

Background of SEMA4A antibody

Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “Semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. The transmembrane semaphorins are characterized by an additional 80 amino acid transmembrane domain and an 80-110 amino acid cytoplasmic domain. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. Semaphorin 4A (SEMA4A), also designated Semaphorin B, is a type I membrane protein. The SEMA4A gene encoding the protein localizes to chromosome 1q22. SEMA4A provides signals to specify territories inaccessible for growing neurons, inhibiting axonal extension.

General readings

Puschel,A.W., et al., (1995) Neuron 14 (5), 941-948

ARP33269_T100 - SEMA4A
Human Jurkat; WB Suggested Anti-SEMA4A Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; SEMA4A antibody - N-terminal region (ARP33269_T100) in Human Jurkat cells using Western Blot
H00064218-B01P - SEMA4A
Western Blot analysis of SEMA4A expression in transfected 293T cell line (H00064218-T01) by SEMA4A MaxPab polyclonal antibody.Lane 1: SEMA4A transfected lysate(83.71 KDa).Lane 2: Non-transfected lysate.
H00064218-D01P - SEMA4A
Western Blot analysis of SEMA4A expression in transfected 293T cell line (H00064218-T02) by SEMA4A MaxPab polyclonal antibody.Lane 1: SEMA4A transfected lysate(83.60 KDa).Lane 2: Non-transfected lysate.
AP20459PU-N - SEMA4A
Immunohistochemistry analysis of SEMA4A antibody Cat.-No. AP20459PU-N in paraffin-embedded human brain tissue.
AP20459PU-N - SEMA4A
Western blot analysis of SEMA4A antibody Cat.-No.: AP20459PU-N in extracts from COS-7 cells.
C11691-1 - SEMA4A
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide.
C11691-1 - SEMA4A
Western blot analysis of extracts from COS7/Jurkat cells, using SEMA4A Antibody. The lane on the right is treated with the synthesized peptide.
AP53846PU-N - SEMA4A
Western blot analysis of SEMA4A Antibody (N-term) Cat.-No AP53846PU-N in Mouse kidney tissue lysates (35ug/lane). This demonstrates the SEMA4A antibody detected the SEMA4A protein (arrow).
AM26491AF-N - SEMA4A
Flow cytometric analysis of Sema4A expression on WEHI-3B: Closed: AM26491AF-N; Open: isotype control
AM26491FC-N - SEMA4A
Flow cytometric analysis of Sema4A expression on WEHI-3B. Closed: AM26491FC-N; Open: isotype control.
AM26491FC-N - SEMA4A
Flow cytometric analysis of Sema4A on human PBMC Cell. R1: lymphocyte, R2: monocyte, R3: granulocyte Antibody. Closed: AM26491FC-N; Open: isotype control.
CPA2752-100ul - SEMA4A
Immunofluorescent analysis of Semaphorin 4A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA2752-100ul - SEMA4A
Western blot analysis of Semaphorin 4A expression in HeLa (A); COS7 (B) whole cell lysates.
CPA2752-100ul - SEMA4A
Immunoprecipitation of Semaphorin 4A from 0.5mg Jurkat whole cell extract lysate using 5ug of Anti-Semaphorin 4A Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70 C; 10ul of each sample was separated on a SDS PAGE gel transferred to a nitrocellulose membrane blocked with 5% BSA and probed with Anti-Semaphorin 4A Antibody.
TP302231 - SEMA4A
TA331773 - SEMA4A
WB Suggested Anti-SEMA4A Antibody Titration: 1.25ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate
TA350396 - SEMA4A
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (SEMA4A Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: ×200)

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SEMA4A antibody
  Rabbit Polyclonal Anti-SEMA4A Antibody  
WB Suggested Anti-SEMA4A Antibody Titration: 1.25ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

SEMA4A antibody
  Rabbit Polyclonal Anti-SEMA4A rabbit polyclonal antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (SEMA4A Antibody) at dilution 1/45, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

SEMA4A antibody
Flow cytometric analysis of Sema4A expression on WEHI-3B: Closed: AM26491AF-N; Open: isotype control Mouse IgG1 5E3 Azide Free Hu, Ms F, IP
0.1 mg / €350.00
  Acris Antibodies GmbH

SEMA4A antibody
Flow cytometric analysis of Sema4A expression on WEHI-3B. Closed: AM26491FC-N; Open: isotype control. Mouse IgG1 5E3 FITC Hu, Ms F
0.1 ml / €350.00
  Acris Antibodies GmbH
+1 additional image

SEMA4A antibody
Immunohistochemistry analysis of SEMA4A antibody Cat.-No. AP20459PU-N in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
+1 additional image

SEMA4A (N-term) antibody
Western blot analysis of SEMA4A Antibody (N-term) Cat.-No AP53846PU-N in Mouse kidney tissue lysates (35ug/lane). This demonstrates the SEMA4A antibody detected the SEMA4A protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SEMA4A antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech
+1 additional image

SEMA4A antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

SEMA4A - human gene knockout kit via CRISPR
SEMA4A - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SEMA4A - human gene knockout kit via CRISPR
SEMA4A - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SEMA4A
  Recombinant protein of human sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A (SEMA4A)  
SEMA4A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

SEMA4A overexpression lysate
SEMA4A overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

SEMA4A overexpression lysate
SEMA4A overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

13 Item(s)

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  2. Monoclonal (2)
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  1. N-term (1)
Reactivity
  1. Human (Hu) (8)
  2. Mouse (Ms) (7)
  3. Rat (Rt) (4)
  4. Canine (Can) (1)
  5. Equine (Eq) (1)
  6. Guinea Pig (GP) (1)
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  1. Western blot / Immunoblot (WB) (5)
  2. Paraffin Sections (P) (4)
  3. Enzyme Immunoassay (E) (3)
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