SEMA4A antibody

Principal name

SEMA4A antibody

Alternative names for SEMA4A antibody

Semaphorin-4A, Semaphorin-B, Sema B, SEMAB, SEMB

SwissProt ID

Q5EA85 (Bovin), Q62178 (Mouse), Q9H3S1 (Human)

Gene ID

64218 (SEMA4A)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of SEMA4A antibody

Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “Semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. The transmembrane semaphorins are characterized by an additional 80 amino acid transmembrane domain and an 80-110 amino acid cytoplasmic domain. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. Semaphorin 4A (SEMA4A), also designated Semaphorin B, is a type I membrane protein. The SEMA4A gene encoding the protein localizes to chromosome 1q22. SEMA4A provides signals to specify territories inaccessible for growing neurons, inhibiting axonal extension.

General readings

Puschel,A.W., et al., (1995) Neuron 14 (5), 941-948

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SEMA4A antibody

WB Suggested Anti-SEMA4A Antibody Titration: 1.25ug/ml; ELISA Titer: 1: 312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SEMA4A antibody

Flow cytometric analysis of Sema4A expression on WEHI-3B: Closed: AM26491AF-N; Open: isotype control Mouse IgG1 5E3 Azide Free Hu, Ms F, IP
0.1 mg / €340.00
  Acris Antibodies GmbH

SEMA4A antibody

Flow cytometric analysis of Sema4A expression on WEHI-3B. Closed: AM26491FC-N; Open: isotype control. Mouse IgG1 5E3 FITC Hu, Ms F
0.1 ml / €340.00
  Acris Antibodies GmbH

SEMA4A antibody

Immunohistochemistry analysis of SEMA4A antibody Cat.-No. AP20459PU-N in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SEMA4A (N-term) antibody

Western blot analysis of SEMA4A Antibody (N-term) Cat.-No AP53846PU-N in Mouse kidney tissue lysates (35ug/lane). This demonstrates the SEMA4A antibody detected the SEMA4A protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SEMA4A antibody

SEMA4A Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SEMA4A antibody

Western Blot analysis of SEMA4A expression in transfected 293T cell line (H00064218-T01) by SEMA4A MaxPab polyclonal antibody.

Lane 1: SEMA4A transfected lysate(83.71 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

SEMA4A antibody

SEMA4A Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

SEMA4A antibody

Western Blot analysis of SEMA4A expression in transfected 293T cell line (H00064218-T02) by SEMA4A MaxPab polyclonal antibody.

Lane 1: SEMA4A transfected lysate(83.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

SEMA4A antibody

SEMA4A Mouse IgG2a 4E2 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

SEMA4A antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

SEMA4A antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SEMA4A Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

SEMA4A antibody

Human Jurkat; WB Suggested Anti-SEMA4A Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; SEMA4A antibody - N-terminal region (ARP33269_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

SEMA4A antibody

Immunofluorescent analysis of Semaphorin 4A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Mky ICC/IF, IP, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SEMA4A antibody

Immunofluorescent analysis of Semaphorin 4A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Mky ICC/IF, IP, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SEMA4A antibody

Immunofluorescent analysis of Semaphorin 4A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Mky ICC/IF, IP, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SEMA4A antibody

SEMA4A Mouse IgG1 HIAT-2 Purified Hu, Ms ELISA, F, IP
25 µg / €700.00
  Funakoshi Co., Ltd.

SEMA4A antibody

Immunohistochemistry: SEMA4A Antibody [NBP1-19614] -  SEMA4A Antibody [NBP1-19614] - paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

SEMA4A antibody

SEMA4A Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

SEMA4A antibody

SEMA4A Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

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