ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of Semenogelin-1 (SEMG1) antibody
The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. The prostate-specific antigen (PSA) protease processes this protein into smaller peptides, with each possibly having a separate function. The proteolysis process breaks down the gel matrix and allows the spermatozoa to move more freely. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (b) is shorter but has the same N- and C-termini compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Brillard-Bourdet M, et al. (2002). Eur J Biochem. 269(1):390-5
Western Blot analysis of SEMG1 expression in transfected 293T cell line (H00006406-T01) by SEMG1 MaxPab polyclonal antibody.Lane 1: SEMG1 transfected lysate(44.22 KDa).Lane 2: Non-transfected lysate.
Human COLO205; WB Suggested Anti-SEMG1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: COLO205 cell lysate. SEMG1 is supported by BioGPS gene expression data to be expressed in COLO205.; SEMG1 antibody - middle region (ARP42089_P050) in Human COLO205 cells using Western Blot