SENP1 antibody

Principal name

SENP1 antibody

Alternative names for SENP1 antibody

Sentrin-specific protease 1, Sentrin/SUMO-specific protease SENP1

SwissProt ID

P59110 (Mouse), Q9P0U3 (Human), Q9P0U3-2

Gene ID

29843 (SENP1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), African clawed frog, Bov (Bovine), Can (Canine), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of SENP1 antibody

SUMO (small ubiquitin-related modifier), a member of the ubiquitin-like protein family, regulates diverse cellular functions of a variety of target proteins including transcription, DNA repair, nucleocytoplasmic trafficking and chromosome segregation. SUMO precursor proteins undergo cleavage of the residues after the “GG” region by SUMO-specific proteases in maturation. This cleavage of the precursor is a prerequisite for subsequent sumoylation. The sentrin-specific (or SUMO-specific) protease (SENP) proteins belong to the peptidase C48 family and include SENP1-3 and SENP5-8. SENP1, SENP2 and SENP3 degrade UBL1 and SMT3H2 conjugates and subsequently release the monomers from sumoylated substrates. HIPK2 is a desumoylation target for SENP1 which shuttles between the cytoplasm and the nucleus. Mutation analyses reveal that SENP1 contains the nuclear export sequence (NES) within the extreme carboxyl-terminal region, and SENP1 is exported to the cytoplasm in a NES-dependent manner. SENP2 has been implicated as a downregulator of CTNNB1 levels and may therefore be a modulator of the Wnt pathway. SUMO protease SENP3 reverses the sumoylation of MEF-2 to augment its transcriptional and myogenic activities. SENP5 localizes to the nucleolus and preferentially processes SUMO-3. It is thought to play a role in mitosis and/or cytokinesis. SENP6 localizes to the cytoplasm and releases SUMO-1. Expression of SENP6 is higher in reproductive organs, indicating that it may mediate processes related to reproduction. SENP8 is involved in the release of sentrins.

General readings

Ohbayashi,N., (2008) Biochem. Biophys. Res. Commun. 371 (4), 823-828

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SENP1 antibody

Western blot analysis of extracts from Jurkat cells, using SENP1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SENP1 antibody

WB Suggested Anti-SENP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

SENP1 antibody

Western blot analysis on Jurkat cell lysate using SENP1 Antibody Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SENP1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SENP1 (RC207194, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SENP1. Mouse IgG1 OTI1D7 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

SENP1 antibody

Immunohistochemistry (IHC) analyzes of SENP1 antibody (Cat.-No.: AP06456PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SENP1 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SENP1 (C-term) antibody

Western blot analysis of anti-SENP1 (C-term)Pab (Cat. #AP11233PU-N)in whole HL60 cell lysate: SENP1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

SENP1 (N-term) antibody

Human Thymus (formalin-fixed, paraffin-embedded) stained with SENP1 antibody AP22748PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

SENP1 antibody

Western Blot analysis of SENP1 expression in transfected 293T cell line (H00029843-T01) by SENP1 MaxPab polyclonal antibody.

Lane 1: SENP1 transfected lysate(70.84 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SENP1 antibody

Western blot analysis of SENP1 in A ) Daudi, B ) HeLa and C ) NIH/3T3 cell lysate. Using SENP1 polyclonal antibody ( Cat # PAB0344 ) at 4 µg/ml. Rabbit Hu, Ms WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

SENP1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the SENP1 polyclonal antibody ( Cat # PAB2411 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SENP1 antibody

Western blot analysis of SENP1 polyclonal antibody ( Cat # PAB2412 ) in whole HL-60 cell lysate : SENP1 ( Arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

SENP1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SENP1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

SENP1 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SENP1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

SENP1 antibody

Human HepG2; WB Suggested Anti-SENP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: HepG2 cell lysate; SENP1 antibody - middle region (ARP55082_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

SENP1 antibody

Immunohistochemical analysis of SENP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Mky P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SENP1 antibody

Immunohistochemical analysis of SENP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Mky P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SENP1 antibody

Immunohistochemical analysis of SENP1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Hu, Mky P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SENP1 antibody

Western Blot: SENP1 Antibody [NBP1-49999] - Detection of Human SENP1 by Western Blot and Immunoprecipitation.  Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-SENP1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  SENP1 was also immunoprecipitated by rabbit anti-SENP1 antibody, which recognizes a downstream epitope.  For blotting immunoprecipitated SENP1, the ReliaBLOT®  Reagents and Procedures were used.   Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

SENP1 antibody

SENP1 Rabbit IgG Aff - Purified Hu, Ms E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

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