Separin / ESPL1 antibody

Principal name

Separin / ESPL1 antibody

Alternative names for Separin / ESPL1 antibody

Separase, ESP1, KIAA0165, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein

SwissProt ID

P60330 (Mouse), Q14674 (Human)

Gene ID

9700 (ESPL1), 105988 (Espl1)

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Dot blot (Dot), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Separin / ESPL1 antibody

Kit Component:
- KN305377G1, Espl1 gRNA vector 1 in pCas-Guide vector
- KN305377G2, Espl1 gRNA vector 2 in pCas-Guide vector
- KN305377D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Chestukhin, A., et al., Proc. Natl. Acad. Sci. U.S.A. 100(8):4574-4579 (2003).
2. Waizenegger, I., et al., Curr. Biol. 12(16):1368-1378 (2002).
3. Chen, F., et al., J. Biol. Chem. 277(19):16775-16781 (2002).
4. Hauf, S., et al., Science 293(5533):1320-1323 (2001).
Zou, H., et al., Science 285(5426):418-422 (1999).

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Separin / ESPL1 antibody

  Rabbit Polyclonal Separase Antibody  
Detection of ESPL1 isoforms 1 and 2 in A431 whole cell lysates using anti-ESPL1. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Separin / ESPL1 antibody

  Mouse Monoclonal Separase Antibody (XJ11-1B12)  
Western Blot: Separase antibody (XJ11-1B12) - Separase detected in 5C6 cell lysates using anti-Separase. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone. Mouse IgG1 XJ11-1B12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Separin / ESPL1 antibody

  Mouse Monoclonal Separase Antibody (XJ11-4D7)  
Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using TA309647, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells. Mouse IgG2a XJ11-4D7 Purified Hu ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

Separin / ESPL1 antibody

  Rabbit Polyclonal Anti-ESPL1 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Raji cells, Primary antibody: (ESPL1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Separin / ESPL1 (1866-1966) antibody

Western blot analysis of Separase expression in HeLa (1), Raji (2), ZR-75-1 (3), MCF7 (4), HL-60 (5) and Jurkat (6) using AM26568AF-N. Mouse IgG1 XJ11-1B12 Azide Free Hu WB
0.1 mg / €370.00
  Acris Antibodies GmbH

Separin / ESPL1 pSer1126 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

Separin / ESPL1 antibody

Immunohistochemistry analyzes of Separase antibody (AP20283PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Separin / ESPL1 antibody

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Separin / ESPL1 pSer801 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

Separin / ESPL1 pSer801 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

Separin / ESPL1 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SEPARASE (Ab-801) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech

Separin / ESPL1 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SEPARASE (Ab-801) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

ESPL1 - human gene knockout kit via CRISPR

ESPL1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ESPL1 - human gene knockout kit via CRISPR

ESPL1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ESPL1 - human gene knockout kit via CRISPR

ESPL1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ESPL1 - human gene knockout kit via CRISPR

ESPL1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Espl1 - mouse gene knockout kit via CRISPR

Espl1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Espl1 - mouse gene knockout kit via CRISPR

Espl1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Espl1 - mouse gene knockout kit via CRISPR

Espl1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Espl1 - mouse gene knockout kit via CRISPR

Espl1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

20 Item(s)

per page
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