Separin / ESPL1 antibody

Principal name

Separin / ESPL1 antibody

Alternative names for Separin / ESPL1 antibody

Separase, ESP1, KIAA0165, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein

SwissProt ID

P60330 (Mouse), Q14674 (Human)

Gene ID

9700 (ESPL1)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Dot blot (Dot), Immunoprecipitation (IP)

Background of Separin / ESPL1 antibody

ESPL1, or separase, is a cysteine protease that is essential for mitotic progression by separating sister chromatids. Each cell must receive one chromatid of every chromosome, during mitosis. Cohesin plays an important role in cohering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys cohesin by means of cleavage, allowing the chromatids to separate and divide with the cell. Separase activity is highly regulated. It not only cleaves cohesin at the onset of anaphase but also cleaves itself, promoting downregulation of separase after anaphase. Should a human cell become an aneuploid (one too many or too few chromatids), the embryo most likely will not survive. Should the embryo survive, it will most likely develop severe birth defects or later develop malignant cancers. Separase antibodies can be used as a specific marker for centrosomes of mitotic cells. The staining of separase in centrosomes can be detected from prophase of mitosis up until anaphase.

General readings

1. Chestukhin, A., et al., Proc. Natl. Acad. Sci. U.S.A. 100(8):4574-4579 (2003).
2. Waizenegger, I., et al., Curr. Biol. 12(16):1368-1378 (2002).
3. Chen, F., et al., J. Biol. Chem. 277(19):16775-16781 (2002).
4. Hauf, S., et al., Science 293(5533):1320-1323 (2001).
Zou, H., et al., Science 285(5426):418-422 (1999).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Separin / ESPL1 antibody

Detection of ESPL1 isoforms 1 and 2 in A431 whole cell lysates using anti-ESPL1. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Separin / ESPL1 antibody

Western Blot: Separase antibody (XJ11-1B12) - Separase detected in 5C6 cell lysates using anti-Separase. Lane 1: Non-induced lysate. Lane 2: Lysate induced with ponasterone. Mouse IgG1 XJ11-1B12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Separin / ESPL1 antibody

Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using TA309647, and gamma tubulin [green] in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells. Mouse IgG2a XJ11-4D7 Purified Hu ICC/IF
0.1 ml / €325.00
  OriGene Technologies, Inc.

Separin / ESPL1 (1866-1966) antibody

Western blot analysis of Separase expression in HeLa (1), Raji (2), ZR-75-1 (3), MCF7 (4), HL-60 (5) and Jurkat (6) using AM26568AF-N. Mouse IgG1 XJ11-1B12 Azide Free Hu WB
0.1 mg / €360.00
  Acris Antibodies GmbH

Separin / ESPL1 pSer1126 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

Separin / ESPL1 antibody

Immunohistochemistry analyzes of Separase antibody (AP20283PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Separin / ESPL1 antibody

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Separin / ESPL1 antibody

Separin / ESPL1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Separin / ESPL1 antibody

Immunoperoxidase of monoclonal antibody to ESPL1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml] Mouse IgG2a 6H6 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Separin / ESPL1 antibody

Overlay ( blue ) of centrosomal staining in HeLa cells. Centrosomal staining of ESPL1 ( yellow ), using ESPL1 monoclonal antibody, clone XJ11-4D7 ( Cat # MAB2347 ), and gamma tubulin ( green ) in mitotic-metaphase cells and nuclear staining of separase in pre-mitotic cells. Mouse IgG2a XJ11-4D7 Hu ICC/IF
0.1 ml / €470.00
  Abnova Taiwan Corp.

Separin / ESPL1 pSer1126 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with ESPL1 (phospho S1126) polyclonal antibody ( Cat # PAB0608 ) which was peroxidase-conjugated to the secondary antibody followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. Rabbit Hu Dot, E, P
0.4 ml / €400.00
  Abnova Taiwan Corp.

Separin / ESPL1 antibody

Western blot analysis of ESPL1 isoforms 1 and 2 in A-431 whole cell lysates using ESPL1 polyclonal antibody ( Cat # PAB12431 ). Rabbit Hu WB
0.1 ml / €400.00
  Abnova Taiwan Corp.

Separin / ESPL1 pSer801 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

Separin / ESPL1 pSer801 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

Separin / ESPL1 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SEPARASE (Ab-801) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

Separin / ESPL1 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SEPARASE (Ab-801) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

Separin / ESPL1 pSer801 antibody

Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

Separin / ESPL1 pSer801 antibody

Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

Separin / ESPL1 pSer801 antibody

Immunohistochemical analysis of Separase (pS801) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

Separin / ESPL1 antibody

Western blot analysis of Separase expression in A431 (A); HuvEc (B) whole cell lysates. Rabbit Aff - Purified Hu ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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