SEPHS1 antibody

Principal name

SEPHS1 antibody

Alternative names for SEPHS1 antibody

Selenide water dikinase 1, Selenophosphate synthase 1, Selenium donor protein 1, SELD, SPS, SPS1

SwissProt ID

O18373 (Drome), P08458 (Yeast), P49903 (Human), Q0VC82 (Bovin), Q6PF47 (Xenla), Q7ZW38 (Danre), Q8BH69 (Mouse)

Gene ID

22929 (SEPHS1), 109079 (Sephs1)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of SEPHS1 antibody

Kit Component:
- KN315520G1, Sephs1 gRNA vector 1 in pCas-Guide vector
- KN315520G2, Sephs1 gRNA vector 2 in pCas-Guide vector
- KN315520D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Chung,H.J., (2006) J. Cell. Physiol. 209 (1), 131-141

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SEPHS1 antibody

  Rabbit polyclonal antibody to Selenophosphate synthetase 1 (selenophosphate synthetase 1)  
Sample(30 ug of whole cell lysate). A:A431. B:MOLT4. 12% SDS PAGE. TA308432 diluted at 1:500 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SEPHS1 antibody

  Rabbit polyclonal Selenophosphate Synthetase 1 antibody  
Western blot using anti-SPS1 antibody shows detection of endogenous SPS1 in NIH3T3 cell. No signal is seen in lysates from cells after pre-treatment with SPS1 siRNA. Endogenous SPS1 can be detected in mouse kidney and liver tissue lysates. Negligible cross-reactivity is seen against recombinant SPS2. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD. Rabbit IgG Serum Hu, Ms, Rt E, WB
0.1 ml / €356.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

SEPHS1 - human gene knockout kit via CRISPR

SEPHS1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

SEPHS1 - human gene knockout kit via CRISPR

SEPHS1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

SEPHS1 - human gene knockout kit via CRISPR

SEPHS1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

SEPHS1 - human gene knockout kit via CRISPR

SEPHS1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sephs1 - mouse gene knockout kit via CRISPR

Sephs1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Sephs1 - mouse gene knockout kit via CRISPR

Sephs1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sephs1 - mouse gene knockout kit via CRISPR

Sephs1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sephs1 - mouse gene knockout kit via CRISPR

Sephs1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

SEPHS1

  Recombinant protein of human selenophosphate synthetase 1 (SEPHS1)  
SEPHS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

SEPHS1 (full length, C-term DDK tag)

  Purified recombinant protein of Human aprataxin and PNKP like factor (APLF), full length, with C-terminal DDK tag, expressed in sf9, 20ug  
SEPHS1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
SF9 cells
20 µg / €399.00
  OriGene Technologies, Inc.

SEPHS1 (full length, N-term HIS tag, transcript variant 3)

  Purified recombinant protein of Human selenophosphate synthetase 1 (SEPHS1), transcript variant 3, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
SEPHS1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

SEPHS1 overexpression lysate

SEPHS1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

SEPHS1 overexpression lysate

SEPHS1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SEPHS1 (mGFP-tagged) - Human selenophosphate synthetase 1 (SEPHS1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, SEPHS1 (mGFP-tagged) - Human selenophosphate synthetase 1 (SEPHS1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, SEPHS1 (mGFP-tagged)-Human selenophosphate synthetase 1 (SEPHS1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, SEPHS1 (mGFP-tagged)-Human selenophosphate synthetase 1 (SEPHS1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, SEPHS1 (mGFP-tagged) - Human selenophosphate synthetase 1 (SEPHS1), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, SEPHS1 (mGFP-tagged) - Human selenophosphate synthetase 1 (SEPHS1), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, SEPHS1 (Myc-DDK tagged) - Human selenophosphate synthetase 1 (SEPHS1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, SEPHS1 (Myc-DDK tagged) - Human selenophosphate synthetase 1 (SEPHS1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, SEPHS1 (Myc-DDK-tagged)-Human selenophosphate synthetase 1 (SEPHS1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, SEPHS1 (Myc-DDK-tagged)-Human selenophosphate synthetase 1 (SEPHS1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

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