Seprase / FAP antibody

Principal name

Seprase / FAP antibody

Alternative names for Seprase / FAP antibody

Fibroblast activation protein alpha, Integral membrane serine protease, 170 kDa melanoma membrane-bound gelatinase

SwissProt ID

P97321 (Mouse), Q12884 (Human)

Gene ID

2191 (FAP), 14089 (Fap)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Mky (Monkey), Hst (Hamster)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P), Immunoprecipitation (IP)

Background of Seprase / FAP antibody

FAP(Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Seprase / FAP antibody

  Rabbit polyclonal Fibroblast Activation Protein antibody  
WB using Anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3).  Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).  The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000.  Personal communication, S.Kim, NCI, Bethesda, MD. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Seprase / FAP antibody

  Rabbit Polyclonal Anti-FAP Antibody  
Host: Rabbit; Target Name: FAP; Sample Tissue: COLO205 Whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Seprase / FAP antibody

  Rabbit Polyclonal Anti-FAP Antibody (Extracellular Domain)  
Anti-FAP antibody IHC of human skin, hair follicle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu, Por
50 µg / €400.00
  OriGene Technologies, Inc.

Seprase / FAP (Extracell. Dom.) antibody

  Rabbit Polyclonal Anti-FAP Antibody (Extracellular Domain)  
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Hu, Mky P
50 µg / €400.00
  OriGene Technologies, Inc.

Seprase / FAP antibody

  Rabbit Polyclonal Anti-FAP-1 Antibody  
Western blot analysis on LOVO cell lysate using FAP-1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Seprase / FAP antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FAP-1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

Seprase / FAP antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FAP-1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

FAP - human gene knockout kit via CRISPR

FAP - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

FAP - human gene knockout kit via CRISPR

FAP - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

FAP - human gene knockout kit via CRISPR

FAP - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fap - mouse gene knockout kit via CRISPR

Fap - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Fap - mouse gene knockout kit via CRISPR

Fap - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fap - mouse gene knockout kit via CRISPR

Fap - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Fap - mouse gene knockout kit via CRISPR

Fap - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Seprase / FAP

  Recombinant protein of human fibroblast activation protein, alpha (FAP)  
Seprase / FAP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ELISA Kits

Catalog No.    

Human Seprase/FAP ELISA Kit

Human Seprase/FAP ELISA Kit  
1 kit / €400.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

FAP overexpression lysate

FAP overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, FAP (mGFP-tagged) - Human fibroblast activation protein, alpha (FAP), 200 uL, >10^7 TU/mL

Lenti ORF particles, FAP (mGFP-tagged) - Human fibroblast activation protein, alpha (FAP), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Fap (GFP-tagged) - Mouse fibroblast activation protein (Fap), 200 uL, >10^7 TU/mL

Lenti ORF particles, Fap (GFP-tagged) - Mouse fibroblast activation protein (Fap), 200 uL, >10^7 TU/mL  
200 µl / €1,460.00
  OriGene Technologies, Inc.

Lenti ORF particles, Fap (Myc-DDK-tagged) - Mouse fibroblast activation protein (Fap), 200 uL, >10^7 TU/mL

Lenti ORF particles, Fap (Myc-DDK-tagged) - Mouse fibroblast activation protein (Fap), 200 uL, >10^7 TU/mL  
200 µl / €1,310.00
  OriGene Technologies, Inc.

20 Item(s)

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