Seprase / FAP antibody

Principal name

Seprase / FAP antibody

Alternative names for Seprase / FAP antibody

Fibroblast activation protein alpha, Integral membrane serine protease, 170 kDa melanoma membrane-bound gelatinase

SwissProt ID

P97321 (Mouse), Q12884 (Human)

Gene ID

2191 (FAP), 14089 (Fap)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Mky (Monkey), Hst (Hamster)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P), Immunoprecipitation (IP)

Background of Seprase / FAP antibody

FAP(Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Seprase / FAP antibody

WB using Anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3).  Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).  The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000.  Personal communication, S.Kim, NCI, Bethesda, MD. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Seprase / FAP antibody

Host: Rabbit; Target Name: FAP; Sample Tissue: COLO205 Whole cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Seprase / FAP antibody

Anti-FAP antibody IHC of human skin, hair follicle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Hu, Por
50 µg / €379.00
  OriGene Technologies, Inc.

Seprase / FAP (Extracell. Dom.) antibody

Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Hu, Mky P
50 µg / €379.00
  OriGene Technologies, Inc.

Seprase / FAP antibody

Human Skin, Dermal Fibroblasts: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Can, Eq, Hst, Hu, Ms, Por, Rb, Rt P
50 µg / €379.00
  OriGene Technologies, Inc.

Seprase / FAP antibody

Western blot analysis on LOVO cell lysate using FAP-1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Seprase / FAP antibody

WB using Anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3).  Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).  The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000.  Personal communication, S.Kim, NCI, Bethesda, MD. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

Seprase / FAP antibody

Western Blot analysis of FAP expression in transfected 293T cell line (H00002191-T01) by FAP MaxPab polyclonal antibody.

Lane 1: FAP transfected lysate(83.6 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Seprase / FAP antibody

Seprase / FAP Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Seprase / FAP antibody

Western Blot analysis of FAP expression in transfected 293T cell line (H00002191-T01) by FAP MaxPab polyclonal antibody.

Lane 1: FAP transfected lysate(87.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Seprase / FAP antibody

FAP monoclonal antibody (M01), clone 1E5 Western Blot analysis of FAP expression in HepG2 ( Cat # L019V1 ). Mouse IgG2a 1E5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Seprase / FAP antibody

Seprase / FAP Mouse IgG2a 2F2 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Seprase / FAP antibody

Seprase / FAP Mouse IgG2a 3C7 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Seprase / FAP antibody

Western blot using Fap polyclonal antibody ( Cat # PAB10209 ) shows detection of Fap protein in whole cell lysates from Fap expressing HEK cells ( lane 4 ), but not control HEK cells ( lane 3 ).
Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide ( lanes 1 and 2 respectively ).
The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP.
Primary antibody was used at 1:1,000.
Personal communication, S.Kim, NCI, Bethesda, MD. Rabbit Hu, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

Seprase / FAP antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FAP-1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

Seprase / FAP antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FAP-1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

Seprase / FAP antibody

Seprase / FAP Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Seprase / FAP

Seprase / FAP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Seprase / FAP

Seprase / FAP Human
2 µg / €300.00
  Abnova Taiwan Corp.

Seprase / FAP

Seprase / FAP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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