SERPINB5 / Maspin antibody

Principal name

SERPINB5 / Maspin antibody

Alternative names for SERPINB5 / Maspin antibody

Serpin B5, PI5, PI-5, Protease inhibitor 5, Peptidase inhibitor 5

SwissProt ID

P36952 (Human), P70124 (Mouse), P70564 (Rat)

Gene ID

5268 (SERPINB5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Rb (Rabbit), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of SERPINB5 / Maspin antibody

Maspin is structurally a serine protease inhibitor (serpin) that was initially isolated from normal human mammary epithelial cells. Serpins are a family of proteins that inhibit chymotrypsin-like serine proteinases. Serpins control activated proteinases and several are involved in the regulation of cell death. Maspin is found in the extracellular matrix and at the plasma membrane. Maspin has been shown to act at the cell surface to block cell motility and inhibit invasion of breast and prostate cancer cells. Maspin is present in normal mammary epithelial cells but is absent in many tumor cell lines, yet no major structural alterations of the Maspin gene have been identified in tumor cells. Similarly, Maspin is expressed in normal prostate cells and downregulated or absent in prostate tumor cells.

General readings

Kim, J., et al. Tumour Biol. 31(5):471-476(2010)
Juengel, E., et al. World J Urol 28(4):465-471(2010)
Besgen, P., et al. J. Immunol. 184(9):5392-5402(2010)
Fung, C.L., et al. Histopathology 56(3):319-330(2010)
Adim, S.B., et al. World J Surg Oncol 8, 22 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SERPINB5 / Maspin antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-SERPINB5 Rabbit Polyclonal antibody. (TA590676) Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SERPINB5 / Maspin antibody

Immunofluorescence analysis: Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green). Mouse IgG2b AT2N6 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

SERPINB5 / Maspin antibody

Immunofluorescence analysis: Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green). Mouse IgG2b AT2N6 Purified Hu E, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

SERPINB5 / Maspin antibody

Formalin-Fixed Paraffin-Embedded Sections of Human breast lobular carcinoma in situ using Maspin Antibody Cat.-No AP09877PU at 7.8-15.625 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Rabbit Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

SERPINB5 / Maspin antibody

Formalin-Fixed Paraffin-Embedded Sections of Human breast lobular carcinoma in situ using Maspin Antibody Cat.-No AP09877PU at 7.8-15.625 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Acris Antibodies GmbH

SERPINB5 / Maspin antibody

Detection of Maspin in HeLa whole cell extracts using anti-Maspin. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SERPINB5 / Maspin antibody

Western blot analysis of extracts from HeLa cells, using Serpin B5 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SERPINB5 / Maspin antibody

Predicted band size: 42 kDa . Positive control: Mouse esophagus and human cervical cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Mouse esophagus tissue lysate Lane2: Human cervical cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 13 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SERPINB5 / Maspin antibody

Predicted band size: 42 kDa . Positive control: Mouse lung tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SERPINB5 / Maspin (Center) antibody

Western blot analysis of lysate from Hela cell line,using Maspin Antibody (Center)(Cat. #TA324471).TA324471 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane. Rabbit Ig Aff - Purified Hu, Ms ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

SERPINB5 / Maspin antibody

Western blot analysis of HT-29 cell and NIH3T3 cell lysate using SERPINB5 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SERPINB5 / Maspin antibody

To detect hMaspin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMaspin. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

SERPINB5 / Maspin antibody

To detect hMaspin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Maspin (TA328370) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SERPINB5 / Maspin antibody

Immunohistochemistry (IHC) analyzes of Maspin antibody (Cat.-No.: AP06801PU-N) on paraffin-embedded human cervix carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

SERPINB5 / Maspin antibody

SERPINB5 / Maspin Rabbit Biotin Hu E, WB
50 µg / €340.00
  Acris Antibodies GmbH

SERPINB5 / Maspin antibody

SERPINB5 / Maspin Rabbit Biotin Hu E, WB
25 µg / €230.00
  Acris Antibodies GmbH

SERPINB5 / Maspin antibody

SERPINB5 / Maspin Rabbit Purified Hu C, E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

SERPINB5 / Maspin antibody

SERPINB5 / Maspin Mouse IgG2a 1F7 Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

SERPINB5 / Maspin antibody

Formalin-fixed, paraffin-embedded human prostate stained with SERPINB5 monoclonal antibody, clone EAW24 ( Cat # MAB3785 ) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and nuclear staining of glandular basal cells. Mouse IgG2a EAW24 Hu P
0.5 ml / €820.00
  Abnova Taiwan Corp.

SERPINB5 / Maspin antibody

Western blot analysis of SERPINB5 in HeLa whole cell extracts with SERPINB5 polyclonal antibody ( Cat# PAB12039 ). Rabbit Hu WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

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