SERPINB5 / Maspin antibody
Principal name
SERPINB5 / Maspin antibody
Alternative names for SERPINB5 / Maspin antibody
Serpin B5, PI5, PI-5, Protease inhibitor 5, Peptidase inhibitor 5
Ncbi ID
NP_002630.2, NP_002630, AAH20713, NP_033283.1, NM_057108, NM_002639
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)
Background of SERPINB5 / Maspin antibody
- Kit Component:
- - KN315587G1, Serpinb5 gRNA vector 1 in pCas-Guide vector
- KN315587G2, Serpinb5 gRNA vector 2 in pCas-Guide vector
- KN315587D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kim, J., et al. Tumour Biol. 31(5):471-476(2010)
Juengel, E., et al. World J Urol 28(4):465-471(2010)
Besgen, P., et al. J. Immunol. 184(9):5392-5402(2010)
Fung, C.L., et al. Histopathology 56(3):319-330(2010)
Adim, S.B., et al. World J Surg Oncol 8, 22 (2010)
Immunohistochemistry (IHC) analyzes of Maspin antibody (Cat.-No.: AP06801PU-N) on paraffin-embedded human cervix carcinoma tissue.
Western blot(WB) analysis of Maspin antibody (Cat.-No.: AP06801PU-N) in extracts from HeLa cells.
Human Skin; Rabbit Anti-SERPINB5 Antibody. Catalog Number: ARP42055. Paraffin Embedded Tissue: Human Skin. Cellular Data: Squamous epithelial cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; SERPINB5 antibody - middle region (ARP42055_T100) in Human Skin cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-SERPINB5 Antibody Titration: 0.625ug/ml. Positive Control: HepG2 cell lysate; SERPINB5 antibody - middle region (ARP42055_T100) in Human HepG2 cells using Western Blot
Recombinant human Maspin, 1-375 aa, His-tagged: 15% SDS-PAGE (3 µg)
Western blot analysis of SERPINB5 in HeLa whole cell extracts with SERPINB5 polyclonal antibody ( Cat# PAB12039 ).
Formalin-fixed, paraffin-embedded human prostate stained with SERPINB5 monoclonal antibody, clone EAW24 ( Cat # MAB3785 ) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and nuclear staining of glandular basal cells.
Immunofluorescence analysis: Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green).
Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green).
Western blot analysis: Cell lysates of HeLa (20 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of HeLa(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Formalin-Fixed Paraffin-Embedded Sections of Human breast lobular carcinoma in situ using Maspin Antibody Cat.-No AP09877PU at 7.8-15.625 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen.
Formalin-Fixed Paraffin-Embedded Sections of Human normal breast in situ using Maspin Antibody Cat.-No AP09877PU at 7.8-15.625 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen.
Formalin-Fixed Paraffin-Embedded Sections of Human breast lobular carcinoma in situ using Maspin Antibody Cat.-No AP09877PU at 7.8-15.625 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen.
Formalin-Fixed Paraffin-Embedded Sections of Human breast lobular carcinoma in situ using Maspin Antibody Cat.-No AP09877PU at 7.8-15.625 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen.
Formalin-Fixed Paraffin-Embedded Sections of Human normal breast in situ using Maspin Antibody Cat.-No AP09877PU at 7.8-15.625 ng/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen.
Western Blot (Reduced) using Maspin Antibody Cat.-No AP09877PU
Western Blot (Unreduced) using Maspin Antibody Cat.-No AP09877PU
Sandwich ELISA using Maspin Antibody Cat.-No AP09877PU
Immunohistochemical analysis of Serpin B5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Serpin B5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Serpin B5 expression in A431 (A); HeLa (B) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-SERPINB5 Rabbit Polyclonal antibody. (TA590676)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-SERPINB5 Rabbit Polyclonal antibody. (TA590676)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB5 (RC224287, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB5.
ELISA: MASPIN Antibody
Detection of Maspin in HeLa whole cell extracts using anti-Maspin.
Western blot analysis of extracts from HeLa cells, using Serpin B5 antibody.The lane on the right is treated with the synthesized peptide.
Predicted band size: 42 kDa . Positive control: Mouse esophagus and human cervical cancer tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 12%SDS-PAGE Lane1: Mouse esophagus tissue lysate Lane2: Human cervical cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 13 seconds)
Predicted cell location: Cytoplasm, nucleus. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using SERPINB5 antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted band size: 42 kDa . Positive control: Mouse lung tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/1000 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)
Predicted cell location: Cytoplasm, nucleus. Positive control: Human esophagus cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using SERPINB5 antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification: x200)
Western blot analysis of lysate from Hela cell line,using Maspin Antibody (Center)(Cat. #TA324471).TA324471 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
Maspin Antibody (Center) (Cat. #TA324471)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Maspin Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of Maspin Antibody (Center)(Cat#TA324471) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of HT-29 cell and NIH3T3 cell lysate using SERPINB5 antibody.
To detect hMaspin by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMaspin.
To detect hMaspin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Maspin (TA328371) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMaspin.
To detect hMaspin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Maspin (TA328370) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin.
To detect hMaspin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Maspin (TA328370) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin.
To detect hMaspin by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Maspin (TA328370) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMaspin.
WB Suggested Anti-SERPINB5 Antibody Titration: 0.625 ug/ml; Positive Control: HepG2 cell lysate
Rabbit Anti-SERPINB5 Antibody; Paraffin Embedded Tissue: Human Skin; Cellular Data: Squamous epithelial cells; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
