SERPINB9 antibody

Principal name

SERPINB9 antibody

Alternative names for SERPINB9 antibody

PI9, PI-9, Serpin B9, Cytoplasmic antiproteinase 3, CAP-3, CAP3, Proteinase inhibitor 9

SwissProt ID

P50453 (Human)

Gene ID

5272 (SERPINB9)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Mouse, Goat, Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of SERPINB9 antibody

SerpinB9 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. The mouse orthologue to PI9 is SPI-6. SerpinB9 was first discovered in human bone marrow in a search for serpins similar to SerpinB6. SerpinB9 was identified in lymphocytes, especially natural killer cells and cytotoxic T lymphocytes, cells which also produce and store the apoptosis-inducing enzyme Granzyme B. PI9 was shown to be a potent inhibitor of Granzyme B, working at picamolar efficiencies. PI9 was also shown to inhibit caspase 1, the interleukin 1 converting enzyme, thus blocking IL1beta production, and decreasing inflammatory processes. Most leukemia cells and many tumor cells produce PI9, leading to speculation that PI9 may help protect tumor cells from destruction by NK and CTL cells. PI9 is also found in placenta, lung, kidney, mast cells, and many tissues and cell lines. In kidney transplant, PI9 is sharply elevated, and in the liver PI9 has been shown to be upregulated by a downstream estrogen promoter. SerpinB9 inhibits Granzyme B, and is cleaved by Granzyme M, as a down regulatory step. SerpinB9 also inhibits the bacterial proteinase subtilisin A, and this may be a protective agent against infections. Neutrophil elastase is also a target of SerpinB9. Like SerpinB6 and SerpinB8, SerpinB9 lacks a signal sequence, and is found mainly in the cytoplasm and nucleus, although it can be detected outside of cells and in serum. The original SerpinB9 sequence described was 379 amino acids in length, with predicted mass of 42.4 kDa and pI of 5.51.

General readings

Jiang X, Ellison SJ, Alarid ET, Shapiro DJ. Interplay between the levels of estrogen and estrogen receptor controls the level of the granzyme inhibitor, proteinase inhibitor 9 and susceptibility to immune surveillance by natural killer cells. Oncogene. 2007 Jan 22; [Epub ahead of print] PMID: 17237823

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

SERPINB9 antibody

TA303212 (0.5µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SERPINB9 antibody

Western blot analysis of extracts from Jurkat cells, using SERPINB9 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

SERPINB9 antibody

Western blot analysis of recombinant PI9: Lane 1: 2 µg Lane 2: 5 µg Lane 3: 10 µg Mouse IgG1 PI9-17 Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

SERPINB9 antibody

SERPINB9 Mouse IgG1 PI9-17 Supernatant Hu ICC/IF, P, WB
1 ml / €420.00
  Acris Antibodies GmbH

SERPINB9 antibody

SERPINB9 Mouse IgG1 7D8 Purified Hu F, P, WB
0.1 mg / €250.00
  Acris Antibodies GmbH

SERPINB9 antibody

SERPINB9 Mouse IgG1 7D8 Purified Hu C, F, WB
0.1 mg / €480.00
  Acris Antibodies GmbH

SERPINB9 antibody

SERPINB9 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

SERPINB9 antibody

Western Blot analysis of SERPINB9 expression in transfected 293T cell line (H00005272-T01) by SERPINB9 MaxPab polyclonal antibody.

Lane 1: SERPINB9 transfected lysate(41.36 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

SERPINB9 antibody

Immunoperoxidase of monoclonal antibody to SERPINB9 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml] Mouse IgG2a 1F5 Purified Hu E, P
0.1 mg / €370.00
  Abnova Taiwan Corp.

SERPINB9 antibody

Western blot analysis using recombinant SERPINB9.
Lane 1 : 2 µg.
Lane 2 : 5 µg.
Lane 3 : 10 µg. Mouse IgG1 PI9-17 Hu C, WB
0.1 mg / €470.00
  Abnova Taiwan Corp.

SERPINB9 antibody

SERPINB9 polyclonal antibody ( Cat # PAB7241 ) ( 0.5 µg/ml ) staining of human placenta lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

SERPINB9 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SERPINB9 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

SERPINB9 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SERPINB9 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

SERPINB9 antibody

Immunohistochemical analysis of Serpin B9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

SERPINB9 antibody

Immunohistochemical analysis of Serpin B9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

SERPINB9 antibody

Immunohistochemical analysis of Serpin B9 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

SERPINB9 antibody

Western Blot: SERPINB9 Antibody [NB100-58965] - NB100-58965 (0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

SERPINB9 antibody

SERPINB9 Mouse IgG1 7D8 Aff - Purified Hu F, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

SERPINB9 antibody

Western Blot: SerpinB9 Antibody [NBP1-45820] - Western blot analysis of recombinant
PI9:
Lane 1: 2 µg
Lane 2: 5 µg
Lane 3: 10 µg Rabbit IgG PI9-17 Aff - Purified Hu WB
0.1 mg / €510.00
  Novus Biologicals Inc.

SERPINB9 antibody

SERPINB9 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

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