Sestrin-2 (SESN2) antibody

Principal name

Sestrin-2 (SESN2) antibody

Alternative names for Sestrin-2 (SESN2) antibody

SESTRIN 2, SEST2, SES2, HI95, Hypoxia induced gene 95

SwissProt ID

P58004 (Human), P58043 (Mouse), Q58CN8 (Bovin)

Gene ID

83667 (SESN2), 230784 (Sesn2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of Sestrin-2 (SESN2) antibody

Sestrin 2 (SESN2), located at human chromosome 1p35.3, belongs to the sestrin family of proteins. These proteins are cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense (1). This family of proteins is only present in multicellular organisms ranging from nematodes to mammals. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes (2). Peroxiredoxins are the enzymes that metabolize peroxides. Expression of these proteins is regulated by p53, a tumor suppressor protein (3). Recently it was reported that sestrin 2 is not a sulfinic peroxiredoxin reductase (4). Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy (5). Depletion of sestrin 2 in human cancer cells reduced the level of autophagy when autophagy was induced in these cells (5).
Similar to sestrin 1, sestrin 2 negatively regulates the activity of mammalian target of rapamycin (mTOR), a positive regulator of cell growth (6). Sestrin 2 may be a promising target in the treatment of chronic obstructive pulmonary disease (COPD). It was reported that mutational inactivation of sestrin 2 induced TGF-β signaling and partially reduced the emphysema phenotype in mice model of COPD (7). Sestrin 2 might also be a key target in the treatment of cancer. It was shown to activate AMPK and repress mTORC1 signaling. AMPK is an essential mediator of LKB1 tumor suppressor and it is down-regulated in cancer cells (8). Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

General readings

1. Meiller A, et al. p53-dependent stimulation of redox-related genes in the lymphoid organs of γ-irradiated mice-identification of Haeme-Oxygenase 1 as a direct p53 target gene. Nucleic Acid Res. November 2007; 35(20):6924-6934.
2. Jonsson TJ, Lowther WT. The peroxiredoxins repair proteins. Subcell Biochem. 2007; 44:115-141.
3. Budanov AV, et al. Regeneration of peroxiredoxins by p53-regulated sestrins, homologs by bacterial AhpD. Science. 23 April 2004; 304(5670):596-600.
4. Woo HA, et al. Sestrin 2 is not a reductase for cytseine sulfinic acid of peroxiredoxins. Antioxid Redox Signal. April 2009; 11(4):739-745.
5. Mairui MC, et al. Stimulation of autophagy by the p53 target gene sestrin 2. Cell Cycle. 15 May 2009; 8(10):1571-1576.
6. Budanov AV, Karin M. p53 target genes sestrin 1 and sestrin 2 connect genotoxic stress and mTOR signaling. Cell. 2008; 134:451-460.
7. Wempe F, et al. Inactivation of sestrin 2 induces TGF-β signaling and partially reduces pulmonary emphysema in a mouse model of COPD. Disease models & mechanisms. 2010; 3:In Press.
8. Shaw RJ. LKB1 and AMP-activated protein kinase control of mTOR signaling and growth. Acta Physiol (Oxf). May 2009; 196(1):65-80.
9. Francesc X. Soriano, Sofia Papadia, Karen F.S. Bell, and Giles E. Hardingham. Role of histone acetylation in the activity-dependent regulation of sulfiredoxin and
sestrin 2. Epigenetics. 2009 April; 4(3): 152–158.

9 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Sestrin-2 (SESN2) antibody

  Rabbit Polyclonal Anti-SESN2 Antibody  
WB Suggested Anti-SESN2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

Sestrin-2 (SESN2) antibody

  Rabbit Polyclonal Anti-SESTRIN2 Antibody  
Western blot analysis of SESTRIN2 in mouse kidney tissue lysate with SESTRIN2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Sestrin-2 (SESN2) antibody

  Rabbit Polyclonal Anti-SESN2 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Lovo cells, hela cells, SKOV3 cells, Primary antibody: (SESN2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Sestrin-2 (SESN2) (Center) antibody

(LEFT) Western blot analysis of Sestrin-2 Antibody (Center) (AP18193PU-N) in 293 cell line lysates (35ug/lane). Sestrin-2 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of SESN2 (arrow) using rabbit polyclonal Sestrin-2 Antibody (Center) (AP18193PU-N). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SESN2 gene (Lane 2). Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SESN2 - human gene knockout kit via CRISPR

SESN2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Sestrin-2 (SESN2)

  Recombinant protein of human sestrin 2 (SESN2)  
Sestrin-2 (SESN2) Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Lysates

Catalog No.    

SESN2 overexpression lysate

SESN2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Sesn2 (GFP-tagged) - Mouse sestrin 2 (Sesn2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Sesn2 (GFP-tagged) - Mouse sestrin 2 (Sesn2), 200 uL, >10^7 TU/mL  
200 µl / €1,100.00
  OriGene Technologies, Inc.

Lenti ORF particles, Sesn2 (Myc-DDK-tagged) - Mouse sestrin 2 (Sesn2), 200 uL, >10^7 TU/mL

Lenti ORF particles, Sesn2 (Myc-DDK-tagged) - Mouse sestrin 2 (Sesn2), 200 uL, >10^7 TU/mL  
200 µl / €980.00
  OriGene Technologies, Inc.

9 Item(s)

per page
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